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    ASAP2 ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 [ Homo sapiens (human) ]

    Gene ID: 8853, updated on 4-Dec-2022

    Summary

    Official Symbol
    ASAP2provided by HGNC
    Official Full Name
    ArfGAP with SH3 domain, ankyrin repeat and PH domain 2provided by HGNC
    Primary source
    HGNC:HGNC:2721
    See related
    Ensembl:ENSG00000151693 MIM:603817; AllianceGenome:HGNC:2721
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    PAP; PAG3; AMAP2; DDEF2; SHAG1; CENTB3; Pap-alpha
    Summary
    This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
    Expression
    Broad expression in testis (RPKM 24.6), kidney (RPKM 11.9) and 21 other tissues See more
    Orthologs
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    See ASAP2 in Genome Data Viewer
    Location:
    2p25.1; 2p24
    Exon count:
    33
    Annotation release Status Assembly Chr Location
    110 current GRCh38.p14 (GCF_000001405.40) 2 NC_000002.12 (9206812..9405678)
    110 current T2T-CHM13v2.0 (GCF_009914755.1) 2 NC_060926.1 (9230598..9430981)
    105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 2 NC_000002.11 (9346941..9545807)

    Chromosome 2 - NC_000002.12Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC105373416 Neighboring gene uncharacterized LOC105373415 Neighboring gene uncharacterized LOC105373417 Neighboring gene Sharpr-MPRA regulatory region 15524 Neighboring gene eukaryotic translation initiation factor 1 pseudogene 7 Neighboring gene Sharpr-MPRA regulatory region 10317 Neighboring gene uncharacterized LOC124907730 Neighboring gene integrin subunit beta 1 binding protein 1 Neighboring gene cleavage and polyadenylation specific factor 3 Neighboring gene isoamyl acetate hydrolyzing esterase 1 (putative) Neighboring gene ADAM metallopeptidase domain 17

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    Phenotypes

    EBI GWAS Catalog

    Description
    Genome-wide association analysis of susceptibility and clinical phenotype in multiple sclerosis.
    EBI GWAS Catalog

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Homology

    Clone Names

    • FLJ42910, KIAA0400

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables GTPase activator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    Process Evidence Code Pubs
    involved_in positive regulation of GTPase activity IEA
    Inferred from Electronic Annotation
    more info
     
    Component Evidence Code Pubs
    located_in Golgi cisterna membrane IEA
    Inferred from Electronic Annotation
    more info
     
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
     

    General protein information

    Preferred Names
    arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2
    Names
    PYK2 C terminus-associated protein
    centaurin, beta 3
    development and differentiation-enhancing factor 2
    paxillin-associated protein with ARF GAP activity 3
    pyk2 C-terminus-associated protein

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001135191.2NP_001128663.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform b

      See identical proteins and their annotated locations for NP_001128663.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The resulting protein (isoform b) is lacking a 45-aa portion of the proline-rich region, compared to isoform a.
      Source sequence(s)
      AA918883, AB007860, AC079782, BC063308
      Consensus CDS
      CCDS46224.1
      UniProtKB/Swiss-Prot
      O43150
      Related
      ENSP00000316404.4, ENST00000315273.4
      Conserved Domains (6) summary
      cd07642
      Location:34248
      BAR_ASAP2; The Bin/Amphiphysin/Rvs (BAR) domain of ArfGAP with SH3 domain, ANK repeat and PH domain containing protein 2
      cd11966
      Location:903958
      SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
      cd13251
      Location:297403
      PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
      sd00045
      Location:586618
      ANK; ANK repeat [structural motif]
      cd08849
      Location:419541
      ArfGap_ASAP2; ArfGAP domain of ASAP2 (ArfGAP2 with SH3 domain, ANK repeat and PH domain-containing protein 2)
      pfam12796
      Location:589673
      Ank_2; Ankyrin repeats (3 copies)
    2. NM_003887.3NP_003878.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform a

      See identical proteins and their annotated locations for NP_003878.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).
      Source sequence(s)
      AA918883, AB007860, AC079782
      Consensus CDS
      CCDS1661.1
      UniProtKB/Swiss-Prot
      D6W4Y8, O43150
      Related
      ENSP00000281419.3, ENST00000281419.8
      Conserved Domains (8) summary
      cd07642
      Location:34248
      BAR_ASAP2; The Bin/Amphiphysin/Rvs (BAR) domain of ArfGAP with SH3 domain, ANK repeat and PH domain containing protein 2
      cd11966
      Location:9481003
      SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
      cd00204
      Location:579673
      ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
      cd13251
      Location:297403
      PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
      pfam00169
      Location:306393
      PH; PH domain
      pfam01412
      Location:421538
      ArfGap; Putative GTPase activating protein for Arf
      pfam13857
      Location:607661
      Ank_5; Ankyrin repeats (many copies)
      sd00045
      Location:586618
      ANK; ANK repeat [structural motif]

    RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 110 details...Open this link in a new tab

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000002.12 Reference GRCh38.p14 Primary Assembly

      Range
      9206812..9405678
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_011510403.4XP_011508705.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X1

      Conserved Domains (8) summary
      cd11966
      Location:9621017
      SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
      cd00204
      Location:593687
      ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
      cd13251
      Location:311417
      PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
      pfam00169
      Location:320407
      PH; PH domain
      pfam01412
      Location:435552
      ArfGap; Putative GTPase activating protein for Arf
      pfam13857
      Location:621675
      Ank_5; Ankyrin repeats (many copies)
      sd00045
      Location:600632
      ANK; ANK repeat [structural motif]
      cl12013
      Location:34259
      BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
    2. XM_011510407.3XP_011508709.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X8

      Conserved Domains (8) summary
      cd11966
      Location:917972
      SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
      cd00204
      Location:593687
      ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
      cd13251
      Location:311417
      PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
      pfam00169
      Location:320407
      PH; PH domain
      pfam01412
      Location:435552
      ArfGap; Putative GTPase activating protein for Arf
      pfam13857
      Location:621675
      Ank_5; Ankyrin repeats (many copies)
      sd00045
      Location:600632
      ANK; ANK repeat [structural motif]
      cl12013
      Location:34259
      BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
    3. XM_011510404.3XP_011508706.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X2

      Conserved Domains (8) summary
      cd11966
      Location:9591014
      SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
      cd00204
      Location:590684
      ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
      cd13251
      Location:308414
      PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
      pfam00169
      Location:317404
      PH; PH domain
      pfam01412
      Location:432549
      ArfGap; Putative GTPase activating protein for Arf
      pfam13857
      Location:618672
      Ank_5; Ankyrin repeats (many copies)
      sd00045
      Location:597629
      ANK; ANK repeat [structural motif]
      cl12013
      Location:34259
      BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
    4. XM_047446201.1XP_047302157.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X9

    5. XM_011510405.4XP_011508707.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X4

      Conserved Domains (8) summary
      cd11966
      Location:937992
      SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
      cd00204
      Location:568662
      ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
      cd13251
      Location:286392
      PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
      pfam00169
      Location:295382
      PH; PH domain
      pfam01412
      Location:410527
      ArfGap; Putative GTPase activating protein for Arf
      pfam13857
      Location:596650
      Ank_5; Ankyrin repeats (many copies)
      sd00045
      Location:575607
      ANK; ANK repeat [structural motif]
      cl12013
      Location:34234
      BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
    6. XM_047446199.1XP_047302155.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X5

    7. XM_047446208.1XP_047302164.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X15

    8. XM_011510406.3XP_011508708.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X7

      Conserved Domains (8) summary
      cd11966
      Location:920975
      SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
      cd00204
      Location:551645
      ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
      cd13251
      Location:269375
      PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
      pfam00169
      Location:278365
      PH; PH domain
      pfam01412
      Location:393510
      ArfGap; Putative GTPase activating protein for Arf
      pfam13857
      Location:579633
      Ank_5; Ankyrin repeats (many copies)
      sd00045
      Location:558590
      ANK; ANK repeat [structural motif]
      cl12013
      Location:11217
      BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
    9. XM_006711898.2XP_006711961.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X3

      Conserved Domains (8) summary
      cd07642
      Location:34248
      BAR_ASAP2; The Bin/Amphiphysin/Rvs (BAR) domain of ArfGAP with SH3 domain, ANK repeat and PH domain containing protein 2
      cd11966
      Location:9511006
      SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
      cd00204
      Location:582676
      ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
      cd13251
      Location:300406
      PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
      pfam00169
      Location:309396
      PH; PH domain
      pfam01412
      Location:424541
      ArfGap; Putative GTPase activating protein for Arf
      pfam13857
      Location:610664
      Ank_5; Ankyrin repeats (many copies)
      sd00045
      Location:589621
      ANK; ANK repeat [structural motif]
    10. XM_006711902.2XP_006711965.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X12

      Conserved Domains (8) summary
      cd07642
      Location:34248
      BAR_ASAP2; The Bin/Amphiphysin/Rvs (BAR) domain of ArfGAP with SH3 domain, ANK repeat and PH domain containing protein 2
      cd11966
      Location:906961
      SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
      cd00204
      Location:582676
      ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
      cd13251
      Location:300406
      PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
      pfam00169
      Location:309396
      PH; PH domain
      pfam01412
      Location:424541
      ArfGap; Putative GTPase activating protein for Arf
      pfam13857
      Location:610664
      Ank_5; Ankyrin repeats (many copies)
      sd00045
      Location:589621
      ANK; ANK repeat [structural motif]
    11. XM_047446200.1XP_047302156.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X6

    12. XM_047446223.1XP_047302179.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X17

    13. XM_011510408.4XP_011508710.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X14

      Conserved Domains (7) summary
      cd00204
      Location:593687
      ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
      cd13251
      Location:311417
      PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
      pfam00169
      Location:320407
      PH; PH domain
      pfam01412
      Location:435552
      ArfGap; Putative GTPase activating protein for Arf
      pfam13857
      Location:621675
      Ank_5; Ankyrin repeats (many copies)
      sd00045
      Location:600632
      ANK; ANK repeat [structural motif]
      cl12013
      Location:34259
      BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
    14. XM_047446219.1XP_047302175.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X16

    15. XM_047446205.1XP_047302161.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X13

    16. XM_047446228.1XP_047302184.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X18

    17. XM_006711901.4XP_006711964.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X10

      Conserved Domains (8) summary
      cd11966
      Location:912967
      SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
      cd00204
      Location:543637
      ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
      cd13251
      Location:261367
      PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
      pfam00169
      Location:270357
      PH; PH domain
      pfam01412
      Location:385502
      ArfGap; Putative GTPase activating protein for Arf
      pfam13857
      Location:571625
      Ank_5; Ankyrin repeats (many copies)
      sd00045
      Location:550582
      ANK; ANK repeat [structural motif]
      cl12013
      Location:3209
      BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
    18. XM_047446202.1XP_047302158.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X11

    19. XM_011510409.1XP_011508711.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X19

      Conserved Domains (8) summary
      cd11966
      Location:833888
      SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
      cd00204
      Location:464558
      ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
      cd13251
      Location:182288
      PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
      pfam00169
      Location:191278
      PH; PH domain
      pfam01412
      Location:306423
      ArfGap; Putative GTPase activating protein for Arf
      pfam13857
      Location:492546
      Ank_5; Ankyrin repeats (many copies)
      sd00045
      Location:471503
      ANK; ANK repeat [structural motif]
      cl12013
      Location:1130
      BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
    20. XM_047446229.1XP_047302185.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X20

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060926.1 Alternate T2T-CHM13v2.0

      Range
      9230598..9430981
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)