ZAP1 Zap1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: ZAP1
Gene description: Zap1p
Primary source: SGD:S000003592
Locus tag: YJL056C
See related: AllianceGenome:SGD:S000003592
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: ZRG10
Summary: Enables DNA-binding transcription factor activity, RNA polymerase II-specific; RNA polymerase II transcription regulatory region sequence-specific DNA binding activity; and zinc ion binding activity. Involved in positive regulation of transcription by RNA polymerase II. Located in nucleus. Orthologous to human MTF1 (metal regulatory transcription factor 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See ZAP1 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (330431..333073, complement)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Changes in transcription start sites of Zap1-regulated genes during zinc deficiency: Implications for HNT1 gene regulation.
Title: Changes in transcription start sites of Zap1-regulated genes during zinc deficiency: Implications for HNT1 gene regulation.
In this study,the authors performed the first phylogenetic classification of shochu strains (Saccharomyces cerevisiae strains) using the nucleotide sequences of genes that reflect genome-level phylogeny. The single-nucleotide polymorphisms information identified in the present study is expected to be useful for distinguishing shochu yeast strains as part of quality control at shochu breweries
Title: Multi-gene phylogenetic analysis reveals that shochu-fermenting Saccharomyces cerevisiae strains form a distinct sub-clade of the Japanese sake cluster.
Zap1 controls UBI4 by inducing transcription from an intragenic promoter, and the resulting truncated mRNA encodes only two of the five ubiquitin repeats.
Title: Activation of the Yeast UBI4 Polyubiquitin Gene by Zap1 Transcription Factor via an Intragenic Promoter Is Critical for Zinc-deficient Growth.
Results suggest that the Zap1 regulon includes ZRG17; Zap1-directed transcriptional regulation has biological importance in maintaining ER function and zinc homeostasis.
Title: Transcriptional regulation of the Zrg17 zinc transporter of the yeast secretory pathway.
Roles of two activation domains in Zap1 in the response to zinc deficiency in Saccharomyces cerevisiae.
Title: Roles of two activation domains in Zap1 in the response to zinc deficiency in Saccharomyces cerevisiae.
through the differential regulation of its target genes, Zap1 prioritizes mechanisms of zinc homeostasis and adaptive responses to zinc deficiency
Title: Differential control of Zap1-regulated genes in response to zinc deficiency in Saccharomyces cerevisiae.
During zinc deficiency, Zap1 mediates the repression of two of the most abundant zinc-requiring enzymes.
Title: Repression of ADH1 and ADH3 during zinc deficiency by Zap1-induced intergenic RNA transcripts.
AD1 (activation domain) residues 332-402 is critical for control of gene expression in response to zinc
Title: Zap1 activation domain 1 and its role in controlling gene expression in response to cellular zinc status.
results describe the solution structure of two zinc fingers (zf1-2(CA3)) derived from a zinc-responsive domain of Zap1 (zf1-2)
Title: Solution structure of a Zap1 zinc-responsive domain provides insights into metalloregulatory transcriptional repression in Saccharomyces cerevisiae.
unusual pattern of ZRT2 regulation among Zap1 target genes involves the antagonistic effect of Zap1 binding to a low-affinity zinc-responsive elements repressor site
Title: The Zap1 transcriptional activator also acts as a repressor by binding downstream of the TATA box in ZRT2.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to zinc ion starvation	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to zinc ion starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Zap1p

NP_012479.1

Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

330431..333073 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181489.1 → NP_012479.1 TPA: Zap1p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012479.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VWC6, P47043

UniProtKB/TrEMBL

A0A8H4C100, N1P8H7

Conserved Domains (4) summary

COG5048
Location:292 → 761

COG5048; FOG: Zn-finger [General function prediction only]

sd00017
Location:770 → 790

ZF_C2H2; C2H2 Zn finger [structural motif]

pfam13465
Location:810 → 834

zf-H2C2_2; Zinc-finger double domain

cd20908
Location:766 → 814

SUF4-like; N-terminal domain of Oryza sativa transcription factor SUPPRESSOR OF FRI 4 (OsSUF4), Arabidopsis thaliana SUF4 (AtSUF4), and similar proteins