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    ROCK1 Rho associated coiled-coil containing protein kinase 1 [ Homo sapiens (human) ]

    Gene ID: 6093, updated on 17-Jun-2024

    Summary

    Official Symbol
    ROCK1provided by HGNC
    Official Full Name
    Rho associated coiled-coil containing protein kinase 1provided by HGNC
    Primary source
    HGNC:HGNC:10251
    See related
    Ensembl:ENSG00000067900 MIM:601702; AllianceGenome:HGNC:10251
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    ROCK-I; P160ROCK
    Summary
    This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015]
    Expression
    Ubiquitous expression in bone marrow (RPKM 22.1), fat (RPKM 16.6) and 25 other tissues See more
    Orthologs
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    See ROCK1 in Genome Data Viewer
    Location:
    18q11.1
    Exon count:
    33
    Annotation release Status Assembly Chr Location
    RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 18 NC_000018.10 (20946906..21111813, complement)
    RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 18 NC_060942.1 (21131976..21296884, complement)
    105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 18 NC_000018.9 (18526867..18691774, complement)

    Chromosome 18 - NC_000018.10Genomic Context describing neighboring genes Neighboring gene ankyrin repeat domain 30B pseudogene Neighboring gene RNA, U6 small nuclear 721, pseudogene Neighboring gene vomeronasal 1 receptor 75 pseudogene Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr18:18511253-18511860 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr18:18511861-18512468 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr18:18512469-18513074 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr18:18513075-18513682 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr18:18516719-18517326 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr18:18519755-18520360 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr18:18519147-18519754 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr18:18518541-18519146 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr18:18517933-18518540 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr18:18517327-18517932 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr18:18516111-18516718 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr18:18515503-18516110 Neighboring gene OCT4-NANOG-H3K27ac hESC enhancer GRCh37_chr18:18514897-18515502 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr18:18514289-18514896 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr18:18513683-18514288 Neighboring gene ReSE screen-validated silencer GRCh37_chr18:18613930-18614117 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr18:18691427-18692015 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 13131 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 13132 Neighboring gene ribosomal protein L21 pseudogene 128 Neighboring gene RNA, U6 small nuclear 120, pseudogene

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    HIV-1 interactions

    Replication interactions

    Interaction Pubs
    HIV-1 infection (VSV-G pseudotyped) of CEMT4 T cells downregulates plasma membrane expression of ROCK1 PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Envelope surface glycoprotein gp120 env Filamin-A-dependent activation of the RhoA-ROCK-LIMK-cofilin pathway is a major event in HIV-1 gp120-induced receptor clustering PubMed
    Tat tat HIV-1 Tat downregulates OCLN (occludin) and LRP1 and upregulates AGER (RAGE), and in a RhoA/ROCK1 dependent manner as shown through hydroxyfasudil inhibition in hCMEC/D3 cells PubMed
    tat HIV-1 Tat-mediated downregulation of occludin expression requires activities of the RhoA and p160-Rho-associated coiled kinase (ROCK) proteins in primary human brain micro vascular endothelial cells PubMed
    matrix gag HIV-1 MA-induced upregulation of collagen-I and alpha-SMA expression is dependent on CXCR2 via the Rho/ROCK-1 pathway PubMed
    gag HIV-1 p17-mediated activation of the Rho/ROCK-signaling pathway is required for p17-mediated monocyte adhesion and migration PubMed
    gag HIV-1 p17-induced activation of PTEN is regulated by RhoA-associated kinase (ROCK) PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Clone Names

    • PRO0435, MGC43611, MGC131603

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables Rho-dependent protein serine/threonine kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables Rho-dependent protein serine/threonine kinase activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables protein kinase activity TAS
    Traceable Author Statement
    more info
    PubMed 
    enables protein serine kinase activity IEA
    Inferred from Electronic Annotation
    more info
     
    enables protein serine/threonine kinase activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables protein serine/threonine kinase activity NAS
    Non-traceable Author Statement
    more info
    PubMed 
    enables protein serine/threonine kinase activity TAS
    Traceable Author Statement
    more info
     
    enables small GTPase binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables tau protein binding NAS
    Non-traceable Author Statement
    more info
    PubMed 
    enables tau-protein kinase activity NAS
    Non-traceable Author Statement
    more info
    PubMed 
    Process Evidence Code Pubs
    involved_in Rho protein signal transduction IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in Rho protein signal transduction IGI
    Inferred from Genetic Interaction
    more info
    PubMed 
    involved_in actin cytoskeleton organization ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in actomyosin structure organization IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in aortic valve morphogenesis ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in apical constriction IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in bleb assembly IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in blood vessel diameter maintenance ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in canonical NF-kappaB signal transduction IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in cortical actin cytoskeleton organization IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in cortical actin cytoskeleton organization IGI
    Inferred from Genetic Interaction
    more info
    PubMed 
    involved_in cortical actin cytoskeleton organization IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in embryonic morphogenesis IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in epithelial to mesenchymal transition ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in leukocyte cell-cell adhesion IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in leukocyte migration IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in leukocyte tethering or rolling IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in mRNA destabilization ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in membrane to membrane docking IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in mitotic cytokinesis IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in motor neuron apoptotic process IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in myoblast migration ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in negative regulation of amyloid precursor protein catabolic process IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in negative regulation of amyloid-beta formation IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in negative regulation of angiogenesis IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in negative regulation of bicellular tight junction assembly IGI
    Inferred from Genetic Interaction
    more info
    PubMed 
    involved_in negative regulation of biomineral tissue development ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in negative regulation of membrane protein ectodomain proteolysis TAS
    Traceable Author Statement
    more info
    PubMed 
    involved_in negative regulation of motor neuron apoptotic process IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in negative regulation of myosin-light-chain-phosphatase activity IGI
    Inferred from Genetic Interaction
    more info
    PubMed 
    involved_in negative regulation of myosin-light-chain-phosphatase activity IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in negative regulation of phosphorylation ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in negative regulation of protein binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in neuron projection arborization IGI
    Inferred from Genetic Interaction
    more info
    PubMed 
    involved_in neuron projection development IGI
    Inferred from Genetic Interaction
    more info
    PubMed 
    involved_in peptidyl-serine phosphorylation IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in peptidyl-threonine phosphorylation IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in podocyte cell migration ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in positive regulation of MAPK cascade IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in positive regulation of amyloid-beta clearance IGI
    Inferred from Genetic Interaction
    more info
    PubMed 
    involved_in positive regulation of autophagy IGI
    Inferred from Genetic Interaction
    more info
    PubMed 
    involved_in positive regulation of cardiac muscle hypertrophy IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in positive regulation of connective tissue replacement IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in positive regulation of dephosphorylation IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in positive regulation of focal adhesion assembly ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in positive regulation of gene expression IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in positive regulation of gene expression ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in positive regulation of phosphatase activity IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in protein localization to plasma membrane IGI
    Inferred from Genetic Interaction
    more info
    PubMed 
    involved_in protein localization to plasma membrane IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in protein phosphorylation IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in regulation of actin cytoskeleton organization IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in regulation of actin cytoskeleton organization ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in regulation of actin cytoskeleton organization TAS
    Traceable Author Statement
    more info
    PubMed 
    involved_in regulation of angiotensin-activated signaling pathway IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in regulation of cell adhesion TAS
    Traceable Author Statement
    more info
    PubMed 
    involved_in regulation of cell junction assembly IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in regulation of cell migration IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in regulation of cell motility TAS
    Traceable Author Statement
    more info
    PubMed 
    involved_in regulation of establishment of cell polarity TAS
    Traceable Author Statement
    more info
    PubMed 
    involved_in regulation of establishment of endothelial barrier IGI
    Inferred from Genetic Interaction
    more info
    PubMed 
    involved_in regulation of establishment of endothelial barrier IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in regulation of focal adhesion assembly TAS
    Traceable Author Statement
    more info
    PubMed 
    involved_in regulation of keratinocyte differentiation IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in regulation of microtubule cytoskeleton organization IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in regulation of neuron differentiation IGI
    Inferred from Genetic Interaction
    more info
    PubMed 
    involved_in regulation of stress fiber assembly TAS
    Traceable Author Statement
    more info
    PubMed 
    involved_in regulation of synapse maturation IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in regulation of synaptic vesicle endocytosis IGI
    Inferred from Genetic Interaction
    more info
    PubMed 
    involved_in response to angiotensin ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in response to transforming growth factor beta ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in signal transduction TAS
    Traceable Author Statement
    more info
    PubMed 
    involved_in smooth muscle contraction TAS
    Traceable Author Statement
    more info
    PubMed 
    Component Evidence Code Pubs
    located_in Golgi membrane IEA
    Inferred from Electronic Annotation
    more info
     
    located_in Schaffer collateral - CA1 synapse IEA
    Inferred from Electronic Annotation
    more info
     
    located_in bleb IEA
    Inferred from Electronic Annotation
    more info
     
    located_in centriole IEA
    Inferred from Electronic Annotation
    more info
     
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    is_active_in cytoplasmic stress granule IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in cytoplasmic stress granule ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    is_active_in cytoskeleton IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in cytoskeleton ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    located_in cytosol TAS
    Traceable Author Statement
    more info
     
    located_in extracellular region TAS
    Traceable Author Statement
    more info
     
    located_in lamellipodium ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    located_in plasma membrane ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    located_in ruffle ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    located_in secretory granule lumen TAS
    Traceable Author Statement
    more info
     

    General protein information

    Preferred Names
    rho-associated protein kinase 1
    Names
    p160 ROCK-1
    renal carcinoma antigen NY-REN-35
    NP_005397.1

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_042178.2 RefSeqGene

      Range
      5039..169946
      Download
      GenBank, FASTA, Sequence Viewer (Graphics), LRG_1005

    mRNA and Protein(s)

    1. NM_005406.3NP_005397.1  rho-associated protein kinase 1

      See identical proteins and their annotated locations for NP_005397.1

      Status: REVIEWED

      Source sequence(s)
      AC036178, AK057038, AU121527, U43195
      Consensus CDS
      CCDS11870.2
      UniProtKB/Swiss-Prot
      B0YJ91, Q13464, Q2KHM4, Q59GZ4
      UniProtKB/TrEMBL
      D9ZGF8
      Related
      ENSP00000382697.1, ENST00000399799.3
      Conserved Domains (7) summary
      cd11639
      Location:489554
      HR1_ROCK1; Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Rho-associated coiled-coil containing protein kinase 1
      smart00220
      Location:76338
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      COG0419
      Location:4221027
      SbcC; DNA repair exonuclease SbcCD ATPase subunit [Replication, recombination and repair]
      cd00029
      Location:12291275
      C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...
      cd01242
      Location:11191225
      PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain
      cd05622
      Location:2406
      STKc_ROCK1; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase 1
      pfam08912
      Location:9491014
      Rho_Binding; Rho Binding

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000018.10 Reference GRCh38.p14 Primary Assembly

      Range
      20946906..21111813 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060942.1 Alternate T2T-CHM13v2.0

      Range
      21131976..21296884 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)