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    PKN2 protein kinase N2 [ Homo sapiens (human) ]

    Gene ID: 5586, updated on 5-May-2024

    Summary

    Official Symbol
    PKN2provided by HGNC
    Official Full Name
    protein kinase N2provided by HGNC
    Primary source
    HGNC:HGNC:9406
    See related
    Ensembl:ENSG00000065243 MIM:602549; AllianceGenome:HGNC:9406
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    PAK2; PRK2; STK7; Pak-2; PRKCL2; PRO2042
    Summary
    Enables RNA polymerase binding activity; histone deacetylase binding activity; and protein serine/threonine kinase activity. Involved in several processes, including apical junction assembly; positive regulation of cell cycle; and positive regulation of viral genome replication. Located in several cellular components, including cleavage furrow; cytoskeleton; and midbody. [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Ubiquitous expression in ovary (RPKM 11.1), duodenum (RPKM 10.9) and 25 other tissues See more
    Orthologs
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    See PKN2 in Genome Data Viewer
    Location:
    1p22.2
    Exon count:
    24
    Annotation release Status Assembly Chr Location
    RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 1 NC_000001.11 (88684273..88836255)
    RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 1 NC_060925.1 (88527688..88679643)
    105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 1 NC_000001.10 (89149956..89301938)

    Chromosome 1 - NC_000001.11Genomic Context describing neighboring genes Neighboring gene PKN2 antisense RNA 1 Neighboring gene ribosomal protein L36a pseudogene 10 Neighboring gene uncharacterized LOC105378838 Neighboring gene Sharpr-MPRA regulatory region 5313 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 1290 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 1291 Neighboring gene uncharacterized LOC124904213 Neighboring gene MED14-independent group 3 enhancer GRCh37_chr1:89239743-89240942 Neighboring gene Sharpr-MPRA regulatory region 874 Neighboring gene uncharacterized LOC124904214 Neighboring gene BRD4-independent group 4 enhancer GRCh37_chr1:89267398-89268597 Neighboring gene RNA, U6 small nuclear 125, pseudogene Neighboring gene elongin C pseudogene 19 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 1293 Neighboring gene general transcription factor IIB Neighboring gene shieldin complex subunit 1 pseudogene Neighboring gene kynurenine aminotransferase 3

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    Phenotypes

    EBI GWAS Catalog

    Description
    Common genetic variation at the IL1RL1 locus regulates IL-33/ST2 signaling.
    EBI GWAS Catalog
    Genome-wide association study identifies loci influencing concentrations of liver enzymes in plasma.
    EBI GWAS Catalog
    Genome-wide association study of periodontal pathogen colonization.
    EBI GWAS Catalog
    Hundreds of variants clustered in genomic loci and biological pathways affect human height.
    EBI GWAS Catalog

    HIV-1 interactions

    Replication interactions

    Interaction Pubs
    Knockdown of protein kinase N2 (PKN2) by siRNA inhibits HIV-1 replication in HeLa P4/R5 cells PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Pr55(Gag) gag The amplified luminescent proximity homogeneous assay (AlphaScreen) identifies the interaction of HIV-1 Gag with PKN2 PubMed
    Vpu vpu In vitro protein-protein interaction analysis identifies a Vpu-binding host protein protein kinase N2 (PKN2) PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Clone Names

    • MGC71074, MGC150606

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables RNA binding HDA PubMed 
    enables RNA polymerase binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables cadherin binding HDA PubMed 
    enables diacylglycerol-dependent serine/threonine kinase activity IEA
    Inferred from Electronic Annotation
    more info
     
    enables histone deacetylase binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables kinase activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables protein kinase activity TAS
    Traceable Author Statement
    more info
    PubMed 
    enables protein serine kinase activity IEA
    Inferred from Electronic Annotation
    more info
     
    enables protein serine/threonine kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables protein serine/threonine kinase activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables small GTPase binding IEA
    Inferred from Electronic Annotation
    more info
     
    Process Evidence Code Pubs
    involved_in apical junction assembly IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in apoptotic process IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in cell adhesion IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in cell division IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in cell projection organization IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in epithelial cell migration IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in intracellular signal transduction IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in peptidyl-serine phosphorylation IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in positive regulation of cytokinesis IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in positive regulation of mitotic cell cycle IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in positive regulation of viral genome replication IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in protein phosphorylation IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in regulation of cell motility IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in signal transduction TAS
    Traceable Author Statement
    more info
    PubMed 
    Component Evidence Code Pubs
    located_in apical junction complex IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in centrosome IDA
    Inferred from Direct Assay
    more info
     
    located_in cleavage furrow IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
     
    located_in cytosol TAS
    Traceable Author Statement
    more info
     
    located_in intermediate filament cytoskeleton IDA
    Inferred from Direct Assay
    more info
     
    located_in lamellipodium IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in midbody IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in nuclear body IDA
    Inferred from Direct Assay
    more info
     
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
     
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in perinuclear region of cytoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
     
    part_of protein-containing complex IEA
    Inferred from Electronic Annotation
    more info
     

    General protein information

    Preferred Names
    serine/threonine-protein kinase N2
    Names
    PKN gamma
    cardiolipin-activated protein kinase Pak2
    protein kinase C-like 2
    protein-kinase C-related kinase 2
    NP_001307636.1
    NP_001307637.1
    NP_001307638.1
    NP_006247.1
    XP_011540074.1
    XP_016857271.1
    XP_047281180.1
    XP_054193636.1
    XP_054193637.1
    XP_054193638.1
    XP_054193639.1
    XP_054193640.1

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001320707.2NP_001307636.1  serine/threonine-protein kinase N2 isoform 2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) lacks an exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.
      Source sequence(s)
      AC119426, AK300304, AL136381, BC125199
      Consensus CDS
      CCDS81350.1
      UniProtKB/Swiss-Prot
      Q16513
      Related
      ENSP00000359544.5, ENST00000370513.9
      Conserved Domains (6) summary
      cd11622
      Location:42107
      HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N
      cd11635
      Location:209282
      HR1_PKN2_3; Third Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N2
      smart00220
      Location:614868
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      cd05589
      Location:609934
      STKc_PKN; Catalytic domain of the Serine/Threonine Kinase, Protein Kinase N
      cl00087
      Location:130201
      HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases
      cl14603
      Location:390472
      C2; C2 domain
    2. NM_001320708.2NP_001307637.1  serine/threonine-protein kinase N2 isoform 3

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region and uses a downstream start codon compared to variant 1. It encodes isoform 3 which has a shorter N-terminus compared to isoform 1.
      Source sequence(s)
      AC119426, AK298595, AL136381, BC125199
      UniProtKB/Swiss-Prot
      Q16513
      Conserved Domains (5) summary
      cd08687
      Location:233316
      C2_PKN-like; C2 domain in Protein kinase C-like (PKN) proteins
      cd11635
      Location:52125
      HR1_PKN2_3; Third Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N2
      smart00220
      Location:505759
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      cd05589
      Location:500825
      STKc_PKN; Catalytic domain of the Serine/Threonine Kinase, Protein Kinase N
      cl00087
      Location:144
      HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases
    3. NM_001320709.2NP_001307638.1  serine/threonine-protein kinase N2 isoform 4

      Status: VALIDATED

      Description
      Transcript Variant: This variant (4) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 4), compared to isoform 1.
      Source sequence(s)
      AC119426, AK298595, AK300304, AL136381
      UniProtKB/Swiss-Prot
      Q16513
      Conserved Domains (6) summary
      cd08687
      Location:364457
      C2_PKN-like; C2 domain in Protein kinase C-like (PKN) proteins
      cd11622
      Location:42107
      HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N
      cd11635
      Location:209282
      HR1_PKN2_3; Third Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N2
      smart00220
      Location:646900
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      cd05589
      Location:641966
      STKc_PKN; Catalytic domain of the Serine/Threonine Kinase, Protein Kinase N
      cl00087
      Location:130201
      HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases
    4. NM_006256.4NP_006247.1  serine/threonine-protein kinase N2 isoform 1

      See identical proteins and their annotated locations for NP_006247.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) encodes the longest isoform (1).
      Source sequence(s)
      AC119426, AL136381, U33052
      Consensus CDS
      CCDS714.1
      UniProtKB/Swiss-Prot
      B4DQ21, B4DTP5, B4DVG1, D3DT24, Q08AF4, Q16513, Q9H1W4
      Related
      ENSP00000359552.3, ENST00000370521.8
      Conserved Domains (5) summary
      cd08687
      Location:390473
      C2_PKN-like; C2 domain in Protein kinase C-like (PKN) proteins
      cd11622
      Location:42107
      HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N
      cd11635
      Location:209282
      HR1_PKN2_3; Third Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N2
      cd05589
      Location:657982
      STKc_PKN; Catalytic domain of the Serine/Threonine Kinase, Protein Kinase N
      cl00087
      Location:130201
      HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000001.11 Reference GRCh38.p14 Primary Assembly

      Range
      88684273..88836255
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_047425224.1XP_047281180.1  serine/threonine-protein kinase N2 isoform X1

    2. XM_017001782.3XP_016857271.1  serine/threonine-protein kinase N2 isoform X2

    3. XM_011541772.3XP_011540074.1  serine/threonine-protein kinase N2 isoform X3

      UniProtKB/Swiss-Prot
      Q16513
      Conserved Domains (3) summary
      cd08687
      Location:64147
      C2_PKN-like; C2 domain in Protein kinase C-like (PKN) proteins
      smart00220
      Location:336590
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      cd05589
      Location:331656
      STKc_PKN; Catalytic domain of the Serine/Threonine Kinase, Protein Kinase N

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060925.1 Alternate T2T-CHM13v2.0

      Range
      88527688..88679643
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054337662.1XP_054193637.1  serine/threonine-protein kinase N2 isoform X1

    2. XM_054337664.1XP_054193639.1  serine/threonine-protein kinase N2 isoform X2

    3. XM_054337663.1XP_054193638.1  serine/threonine-protein kinase N2 isoform X4

    4. XM_054337661.1XP_054193636.1  serine/threonine-protein kinase N2 isoform X1

    5. XM_054337665.1XP_054193640.1  serine/threonine-protein kinase N2 isoform X3