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    MX2 MX dynamin like GTPase 2 [ Homo sapiens (human) ]

    Gene ID: 4600, updated on 3-Nov-2024

    Summary

    Official Symbol
    MX2provided by HGNC
    Official Full Name
    MX dynamin like GTPase 2provided by HGNC
    Primary source
    HGNC:HGNC:7533
    See related
    Ensembl:ENSG00000183486 MIM:147890; AllianceGenome:HGNC:7533
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    MXB
    Summary
    The protein encoded by this gene has a nuclear and a cytoplasmic form and is a member of both the dynamin family and the family of large GTPases. The nuclear form is localized in a granular pattern in the heterochromatin region beneath the nuclear envelope. A nuclear localization signal (NLS) is present at the amino terminal end of the nuclear form but is lacking in the cytoplasmic form due to use of an alternate translation start codon. This protein is upregulated by interferon-alpha but does not contain the antiviral activity of a similar myxovirus resistance protein 1. [provided by RefSeq, Jul 2008]
    Expression
    Broad expression in spleen (RPKM 16.5), appendix (RPKM 13.6) and 23 other tissues See more
    Orthologs
    NEW
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    Try the new Transcript table

    Genomic context

    See MX2 in Genome Data Viewer
    Location:
    21q22.3
    Exon count:
    18
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 21 NC_000021.9 (41362027..41409393)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 21 NC_060945.1 (39750638..39798083)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 21 NC_000021.8 (42733954..42781320)

    Chromosome 21 - NC_000021.9Genomic Context describing neighboring genes Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr21:42551642-42552451 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr21:42572358-42572858 Neighboring gene beta-secretase 2 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr21:42572859-42573359 Neighboring gene placenta enriched 4 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr21:42594561-42595060 Neighboring gene ReSE screen-validated silencer GRCh37_chr21:42612073-42612217 Neighboring gene NANOG hESC enhancer GRCh37_chr21:42616252-42616757 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr21:42646500-42647699 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr21:42651763-42652318 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr21:42652319-42652873 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr21:42661864-42662364 Neighboring gene FAM3 metabolism regulating signaling molecule B Neighboring gene ATAC-STARR-seq lymphoblastoid active region 18470 Neighboring gene OCT4-NANOG-H3K4me1 hESC enhancer GRCh37_chr21:42731293-42732096 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 18471 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 18472 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 13330 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 18473 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr21:42739613-42740114 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr21:42740714-42741402 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 18474 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr21:42741403-42742089 Neighboring gene melanoma risk locus-associated MPRA allelic enhancer 21:42745414 Neighboring gene melanoma risk locus-associated MPRA allelic enhancers 21:42746568 and 21:42746578 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr21:42750497-42750998 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr21:42750999-42751498 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 13332 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 18477 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 18479 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 18478 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr21:42824122-42825321 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr21:42830117-42830618 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 18480 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 18481 Neighboring gene MX dynamin like GTPase 1 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr21:42851654-42852154 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr21:42852155-42852655 Neighboring gene transmembrane protease serine 2 breakpoint cluster recombination region Neighboring gene transmembrane serine protease 2 Neighboring gene TMPRSS2 promoter region

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    HIV-1 interactions

    Replication interactions

    Interaction Pubs
    HIV-1 replication is restricted by functional MX2 (MxB) which is dependent upon amino acid G208 in CA in Cf2Th and THP-1 cells PubMed
    HIV-1 replication is inhibited by wild type MX2 (MxB) but not when mutations in the stalk oligomerization interfaces, hinge region, or L4 loop are made PubMed
    HIV-1 replication is restricted by wild type MX2 (MxB); restriction is dependent on amino acids M574, Y651, and F647 in MX2 as assayed in U87-MG CD4+ CXCR4+ cells PubMed
    HIV-1 replication is NOT restricted by MX2 (MxB) mutants M574D, Y651D and M567D/L570D (mutations affect MX2 dimerization); this observation indicates that MX2 (MxB) dimerization is important for anti-HIV-1 activity in this context PubMed
    HIV-1 replication is restricted by MX2 mutants I423D, K663D, M666D, and the I423/K663D double mutant (all interface 1 MX2 (MxB) mutants); indicates that MX2 oligomerization through interface 1 is not critical for MX2-mediated HIV-1 restriction PubMed
    HIV-1 replication is restricted by MX2 (MxB) mutants E681A and R689A (in HOS cells), indicating anti-HIV-1 activity of MX2 (MxB) is not dependent on transfer of information from the GTPase domain to the stalk domain PubMed
    HIV-1 infection is restricted by wild type MX2 (MxB) and the YRGK487-490AAAA mutant MX2 (MxB) PubMed
    VSVG-pseudotyped recombinant HIV-1 is restricted by MX2 (MxB) with mutations in interface 1 (E424R, K663D, L669D) just as strongly as wild-type MX2 (MxB) PubMed
    Knockdown of MX2 by shRNA enhances HIV-1 replication in an IFN-alpha-dependent manner in HIV-1-infected cells PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Envelope surface glycoprotein gp120 env HIV-1 gp120-treated vaginal epithelial cells show upregulation of myxovirus resistance 2 (MX2, MXB) expression as compared to untreated control PubMed
    Tat tat HIV-1 and the viral protein Tat modulate the expression of MX dynamin-like GTPase 2 (MX2; MxB) in immature dendritic cells and monocyte-derived macrophages PubMed
    Vpr vpr HIV-1 Vpr upregulates the gene expression of MX2 in human monocyte-derived macrophages PubMed
    vpr HIV-1 Vpr upregulates the gene expression of MX2 in human monocyte-derived dendritic cells PubMed
    capsid gag HIV-1 CA binds MX2 (MxB) dependent upon G208 in CA, which is not related to IFN-alpha restriction in THP-1, HT-1080, and U87 MG cell lines PubMed
    gag HIV-1 CA H87P, G116A, and P207A mutants are resistant to inhibition by MX2 (MxB), but still interact with MX2 (MxB) PubMed
    gag CA-NC complex/core (in vitro-assembled from HIV-1 NL4-3 Gag amino acids 133-433; fusion protein) DOES NOT bind to MX2 (MxB) with mutations in interface 2 (Q644D, M574D, Y651D and M574D/Y651D, each preventing MX2 oligomerization) PubMed
    gag CA binding to MX2 (MxB) involves the N-terminal 25 amino acids of MX2 (MxB) PubMed
    gag Anti-HIV-1 activity of MX2 is greatly enhanced when MX2 is directly targeted to HIV-1 CA by fusion with PPIA (CypA) PubMed
    gag CA-NC complex/core (in vitro-assembled from HIV-1 NL4-3 Gag amino acids 133-433; fusion protein) binds to MX2 (MxB) with mutations in interface 1 (E424R, K663D, L669D) as strongly as wild-type MX2 (MxB) PubMed
    gag Disruption of the leucine zipper by a single point mutation L661K abolishes the ability of MX2 to oligomerize, leading to neither binding to in vitro assembled HIV-1 CA-NC complexes nor restrict HIV-1 infection PubMed
    gag MX2 binds to in vitro assembled HIV-2 and SIVmac CA-NC complexes PubMed
    gag HIV-1 CA tubes bind MX2 (MxB) directly in vitro, indicating that cellular cofactors are not required for the direct interaction of these two proteins PubMed
    gag WT CA binds MX2 (MX2-HA) efficiently as well as interface 1 mutants K663D, I423D/K663D, and M666D; hinge 1 mutant E681A and interface 2 mutants M574D and Y651D do not bind CA efficiently PubMed
    gag HIV-1 CA in assembled lattices bind MX2, but not single CA hexamers (pentamers or monomers) PubMed
    gag HIV-1 CA binds MBP-MX2-YRGK/IKM directly, indicating that cellular cofactors are not required for the binding between CA and MX2 PubMed
    gag HIV-1 CA-MX2 interaction plays a role in in restriction of HIV-1 replication (negative correlation of CA-MX2 binding scatterplot analysis yields a Spearman rank correlation coefficient p value of 0.028) PubMed
    gag Efficient HIV-1 CA binding to MX2 requires the first 84 amino acids of MX2 (N-terminus) PubMed
    gag CA/MX2 (MxB) binding requires an intact 'RRR' motif located at amino acids 11-13 in the N-terminal of MX2 (MxB) PubMed
    gag MX2 binds to the HIV-1 core and inhibits the uncoating process of HIV-1 during infection, which is prevented by a benzimidazole-based inhibitor that binds to a specific pocket in the HIV-1 capsid PubMed
    gag HIV-1 CA N57S, K70R, N74D, A88T, G89V, P90A, and T107N mutants are resistant to inhibition by MX2, suggesting that CA is a specific target of MX2 PubMed
    gag HIV-1 CA mutants V86A, V86Q, H87P, H87Q, A88V, and A92P in the cyclophilin A-binding loop of CA are resistant to MxB restriction PubMed
    gag HIV-1 CA mutants E45A, N57A, N57S, K70R, G89V, and T107N are significantly less sensitive than the wild-type to MxB restriction PubMed
    gag MX2 binds to in vitro assembled HIV-1 CA-NC complexes, whereas MX1 is not able to interact with in vitro assembled HIV-1 CA-NC complexes PubMed
    gag MX2 co-localizes with HIV-1 CA, suggesting that MX2 directly associates with HIV-1 cores in living cells PubMed
    gag A protein chimera MX2(1-90)-MX1(43-662) has the ability to bind in vitro assembled HIV-1 CA-NC complexes, suggesting that the N-terminal region (residues 1-90) of MX2 is involved in its ability to bind capsid PubMed
    gag The cryo-EM images show distinct protein densities decorating the HIV-1 CA tubes in the presence of MX2 compared to MX2(84-715) mutant, confirming both direct protein binding and the importance of the MX2 N terminus in this interaction PubMed
    gag HIV-1 CA mutants M10I, N57S, H87R, P90T, Q95L, M185I, P207S, G208R, and T210K are entirely or partially resistant to human MX2 PubMed
    integrase gag-pol HIV-1 IN mutant D64N/D116N is significantly more resistant than the wild-type and D167K IN mutant to MxB restriction PubMed
    nucleocapsid gag CA-NC complex/core (in vitro-assembled from HIV-1 NL4-3 Gag amino acids 133-433; fusion protein) DOES NOT bind to MX2 (MxB) with the L661K mutation (prevents oligomerization) in the putative leucine zipper region PubMed
    gag CA-NC complex/core (in vitro-assembled from HIV-1 NL4-3 Gag amino acids 133-433; fusion protein) DOES NOT bind to MX2 (MxB) with mutations in interface 2 (Q644D, M574D, Y651D and M574D/Y651D, each preventing MX2 oligomerization) PubMed
    gag CA-NC complexes (in vitro-assembled from HIV-1 NL4-3 Gag amino acids 133-433; fusion protein) bind to MX2 (MxB) with mutations in interface 1 (E424R, K663D, L669D) as strongly as wild-type MX2 (MxB) PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Clone Names

    • FLJ44119

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables GTP binding IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    enables GTPase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables microtubule binding IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    Process Evidence Code Pubs
    involved_in defense response TAS
    Traceable Author Statement
    more info
    PubMed 
    involved_in defense response to virus IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in defense response to virus IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in innate immune response IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in mRNA transport IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in protein transport IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in regulation of cell cycle IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in regulation of nucleocytoplasmic transport IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in response to interferon-alpha IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in response to virus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in cytosol TAS
    Traceable Author Statement
    more info
     
    is_active_in membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    is_active_in microtubule IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    part_of nuclear pore IDA
    Inferred from Direct Assay
    more info
    PubMed 
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
     

    General protein information

    Preferred Names
    interferon-induced GTP-binding protein Mx2
    Names
    interferon-regulated resistance GTP-binding protein MXB
    myxovirus (influenza virus) resistance 2
    myxovirus resistance protein 2
    p78-related protein
    second interferon-induced protein p78

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_002463.2NP_002454.1  interferon-induced GTP-binding protein Mx2

      See identical proteins and their annotated locations for NP_002454.1

      Status: REVIEWED

      Source sequence(s)
      AL773578, M30818
      Consensus CDS
      CCDS13672.1
      UniProtKB/Swiss-Prot
      B7Z5D3, D3DSI7, P20592
      Related
      ENSP00000333657.3, ENST00000330714.8
      Conserved Domains (3) summary
      smart00302
      Location:621709
      GED; Dynamin GTPase effector domain
      cd08771
      Location:116387
      DLP_1; Dynamin_like protein family includes dynamins and Mx proteins
      pfam01031
      Location:307593
      Dynamin_M; Dynamin central region

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000021.9 Reference GRCh38.p14 Primary Assembly

      Range
      41362027..41409393
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_011529573.3XP_011527875.1  interferon-induced GTP-binding protein Mx2 isoform X2

      Conserved Domains (3) summary
      smart00302
      Location:576664
      GED; Dynamin GTPase effector domain
      cd08771
      Location:116342
      DLP_1; Dynamin_like protein family includes dynamins and Mx proteins
      pfam01031
      Location:263549
      Dynamin_M; Dynamin central region
    2. XM_024452080.2XP_024307848.1  interferon-induced GTP-binding protein Mx2 isoform X1

      UniProtKB/Swiss-Prot
      B7Z5D3, D3DSI7, P20592
      Conserved Domains (3) summary
      smart00302
      Location:621709
      GED; Dynamin GTPase effector domain
      cd08771
      Location:116387
      DLP_1; Dynamin_like protein family includes dynamins and Mx proteins
      pfam01031
      Location:307593
      Dynamin_M; Dynamin central region
    3. XM_047440780.1XP_047296736.1  interferon-induced GTP-binding protein Mx2 isoform X1

      UniProtKB/Swiss-Prot
      B7Z5D3, D3DSI7, P20592
    4. XM_047440779.1XP_047296735.1  interferon-induced GTP-binding protein Mx2 isoform X1

      UniProtKB/Swiss-Prot
      B7Z5D3, D3DSI7, P20592
    5. XM_005260983.6XP_005261040.1  interferon-induced GTP-binding protein Mx2 isoform X1

      See identical proteins and their annotated locations for XP_005261040.1

      UniProtKB/Swiss-Prot
      B7Z5D3, D3DSI7, P20592
      Related
      ENSP00000506423.1, ENST00000680862.1
      Conserved Domains (3) summary
      smart00302
      Location:621709
      GED; Dynamin GTPase effector domain
      cd08771
      Location:116387
      DLP_1; Dynamin_like protein family includes dynamins and Mx proteins
      pfam01031
      Location:307593
      Dynamin_M; Dynamin central region
    6. XM_047440781.1XP_047296737.1  interferon-induced GTP-binding protein Mx2 isoform X2

    7. XM_005260984.2XP_005261041.1  interferon-induced GTP-binding protein Mx2 isoform X1

      See identical proteins and their annotated locations for XP_005261041.1

      UniProtKB/Swiss-Prot
      B7Z5D3, D3DSI7, P20592
      Conserved Domains (3) summary
      smart00302
      Location:621709
      GED; Dynamin GTPase effector domain
      cd08771
      Location:116387
      DLP_1; Dynamin_like protein family includes dynamins and Mx proteins
      pfam01031
      Location:307593
      Dynamin_M; Dynamin central region
    8. XM_047440782.1XP_047296738.1  interferon-induced GTP-binding protein Mx2 isoform X2

    9. XM_047440783.1XP_047296739.1  interferon-induced GTP-binding protein Mx2 isoform X2

    10. XM_011529572.3XP_011527874.1  interferon-induced GTP-binding protein Mx2 isoform X1

      See identical proteins and their annotated locations for XP_011527874.1

      UniProtKB/Swiss-Prot
      B7Z5D3, D3DSI7, P20592
      Conserved Domains (3) summary
      smart00302
      Location:621709
      GED; Dynamin GTPase effector domain
      cd08771
      Location:116387
      DLP_1; Dynamin_like protein family includes dynamins and Mx proteins
      pfam01031
      Location:307593
      Dynamin_M; Dynamin central region

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060945.1 Alternate T2T-CHM13v2.0

      Range
      39750638..39798083
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054324511.1XP_054180486.1  interferon-induced GTP-binding protein Mx2 isoform X2

    2. XM_054324508.1XP_054180483.1  interferon-induced GTP-binding protein Mx2 isoform X1

      UniProtKB/Swiss-Prot
      B7Z5D3, D3DSI7, P20592
    3. XM_054324509.1XP_054180484.1  interferon-induced GTP-binding protein Mx2 isoform X1

      UniProtKB/Swiss-Prot
      B7Z5D3, D3DSI7, P20592
    4. XM_054324504.1XP_054180479.1  interferon-induced GTP-binding protein Mx2 isoform X1

      UniProtKB/Swiss-Prot
      B7Z5D3, D3DSI7, P20592
    5. XM_054324510.1XP_054180485.1  interferon-induced GTP-binding protein Mx2 isoform X2

    6. XM_054324507.1XP_054180482.1  interferon-induced GTP-binding protein Mx2 isoform X1

      UniProtKB/Swiss-Prot
      B7Z5D3, D3DSI7, P20592
    7. XM_054324505.1XP_054180480.1  interferon-induced GTP-binding protein Mx2 isoform X1

      UniProtKB/Swiss-Prot
      B7Z5D3, D3DSI7, P20592
    8. XM_054324512.1XP_054180487.1  interferon-induced GTP-binding protein Mx2 isoform X2

    9. XM_054324513.1XP_054180488.1  interferon-induced GTP-binding protein Mx2 isoform X2

    10. XM_054324506.1XP_054180481.1  interferon-induced GTP-binding protein Mx2 isoform X1

      UniProtKB/Swiss-Prot
      B7Z5D3, D3DSI7, P20592