Dref DNA replication-related element factor [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Drefprovided by FlyBase
Official Full Name: DNA replication-related element factorprovided by FlyBase
Primary source: FLYBASE:FBgn0015664
Locus tag: Dmel_CG5838
See related: AllianceGenome:FB:FBgn0015664
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG5838; dDREF; DmDREF; Dmel\CG5838; Dmel_DREF; dref; DREF; p80; p80/DREF
Summary: Enables transcription cis-regulatory region binding activity. Contributes to RNA polymerase II core promoter sequence-specific DNA binding activity. Involved in several processes, including DNA endoreduplication; regulation of cellular macromolecule biosynthetic process; and sensory organ development. Located in nucleus and polytene chromosome. Part of transcription regulator complex. Is expressed in several structures, including cuboidal/columnar epithelium; extended germ band embryo; eye disc; gonad; and prothoracic gland. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

Location:: 30F2-30F3; 2-38 cM

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (9964147..9967229, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (9964147..9967229, complement)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Results of genetic interaction analyses revealed that Mi-2 and Caf1/p55, components of the Nucleosome Remodeling and Deacetylase (NuRD) complex, genetically antagonize the role of DREF in germline stem cell maintenance. Taken together, these data suggest that DREF contributes to intrinsic components of the germline stem cell regulatory network that maintains competence to self-renew.
Title: DREF Genetically Counteracts Mi-2 and Caf1 to Regulate Adult Stem Cell Maintenance.
DREF is now emerging as not only a transcription factor, but also a multi-functional protein. In this review, we summarize current advances in studies on the novel functions of Drosophila DREF.
Title: DREF plays multiple roles during Drosophila development.
Together, these results indicate that the DRE/DREF pathway is required for transcriptional activation of the hpo gene to positively control Hippo pathways.
Title: The Hippo pathway as a target of the Drosophila DRE/DREF transcriptional regulatory pathway.
Results show that regulation of e2f1 and PCNA by DREF in vivo is complex and the regulation mechanism may differ with the tissue and/or positions in the tissue.
Title: Control of e2f1 and PCNA by Drosophila transcription factor DREF.
expression of the dref gene occurs in clusters and is temporally regulated at specific times during bristle development.
Title: Temporal and spatial pattern of dref expression during Drosophila bristle development.
DREF proteins may help maintain chromosome organization during the cell cycle and mark a subset of genomic sites for the assembly of pre-replication complexes and gene bookmarking during the M/G1 transition.
Title: Dynamic changes in the genomic localization of DNA replication-related element binding factor during the cell cycle.
DREF binds directly upstream of Prat and the DRE sites associated with its activity are necessary for Prat expression
Title: Transcriptional regulation of the purine de novo synthesis gene Prat in Drosophila melanogaster.
suggest DREF is an essential transcription factor in the nutritional control of cell and tissue growth during Drosophila development.
Title: DREF is required for cell and organismal growth in Drosophila and functions downstream of the nutrition/TOR pathway.
Caudal-related homeodomain proteins CDX1/2 bind to DNA replication-related element binding factor.
Title: Caudal-related homeodomain proteins CDX1/2 bind to DNA replication-related element binding factor.
The results provide evidence that DREF plays critical roles, especially in endoreplication process of bristle development, at least in part by regulating the pcna gene expression.
Title: DREF is critical for Drosophila bristle development by regulating endoreplication in shaft cells.

Submit: New GeneRIF Correction See all GeneRIFs (29)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
contributes_to RNA polymerase II core promoter sequence-specific DNA binding	IPI Inferred from Physical Interaction more info	PubMed
enables calcium ion binding	ISS Inferred from Sequence or Structural Similarity more info
enables transcription cis-regulatory region binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA endoreduplication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chaeta development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in compound eye development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in compound eye morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dendrite morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein autophosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein phosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of DNA biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of apoptotic process	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of cell proliferation involved in compound eye morphogenesis	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of mitochondrial depolarization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sensory organ precursor cell division	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in polytene chromosome	IDA Inferred from Direct Assay more info	PubMed
part_of transcription regulator complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: DNA replication-related element factor

Names: CG5838-PA; CG5838-PB; DNA; DNA replication-related element binding factor; DNA replication-related element-binding factor; DNA-replication-related element factor; DRE (DNA replication-related element) binding factor; DRE-binding factor; Dref-PA; Dref-PB; replication-related element-binding factor

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

9964147..9967229 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_078805.4 → NP_523529.1 DNA replication-related element factor, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_523529.1

Status: REVIEWED

UniProtKB/TrEMBL

Q86NK7, Q94883

Related

FBpp0079485

Conserved Domains (1) summary

pfam02892
Location:35 → 84

zf-BED; BED zinc finger
NM_001273382.2 → NP_001260311.1 DNA replication-related element factor, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001260311.1

Status: REVIEWED

UniProtKB/TrEMBL

Q86NK7, Q94883

Conserved Domains (1) summary

pfam02892
Location:35 → 84

zf-BED; BED zinc finger