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    MED4 mediator complex subunit 4 [ Homo sapiens (human) ]

    Gene ID: 29079, updated on 5-Mar-2024

    Summary

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    Official Symbol
    MED4provided by HGNC
    Official Full Name
    mediator complex subunit 4provided by HGNC
    Primary source
    HGNC:HGNC:17903
    See related
    Ensembl:ENSG00000136146 MIM:605718; AllianceGenome:HGNC:17903
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    ARC36; VDRIP; DRIP36; TRAP36; HSPC126
    Summary
    This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
    Expression
    Ubiquitous expression in testis (RPKM 13.4), bone marrow (RPKM 12.6) and 25 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    Location:
    13q14.2
    Exon count:
    7
    Annotation release Status Assembly Chr Location
    RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 13 NC_000013.11 (48075724..48095104, complement)
    RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 13 NC_060937.1 (47296104..47315464, complement)
    105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 13 NC_000013.10 (48649860..48669240, complement)

    Chromosome 13 - NC_000013.11Genomic Context describing neighboring genes Neighboring gene succinate-CoA ligase ADP-forming subunit beta Neighboring gene ATAC-STARR-seq lymphoblastoid active region 7717 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 7718 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 7719 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 5330 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr13:48575750-48576949 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr13:48577621-48578218 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr13:48578817-48579414 Neighboring gene uncharacterized LOC124903172 Neighboring gene OCT4-NANOG-H3K27ac hESC enhancer GRCh37_chr13:48610990-48611866 Neighboring gene OCT4-NANOG-H3K27ac hESC enhancer GRCh37_chr13:48611867-48612742 Neighboring gene nudix hydrolase 15 Neighboring gene MED14-independent group 3 enhancer GRCh37_chr13:48640162-48641361 Neighboring gene MED14-independent group 3 enhancer GRCh37_chr13:48646101-48647300 Neighboring gene Sharpr-MPRA regulatory region 5997 Neighboring gene MED4 antisense RNA 1 Neighboring gene uncharacterized LOC105370198 Neighboring gene RNA polymerase II subunit K pseudogene 2 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 7723 Neighboring gene NANOG-H3K27ac hESC enhancer GRCh37_chr13:48733841-48734451 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 7724 Neighboring gene Sharpr-MPRA regulatory region 1366 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 5331 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 5332 Neighboring gene NANOG-H3K27ac hESC enhancer GRCh37_chr13:48807853-48808550 Neighboring gene OCT4-NANOG hESC enhancer GRCh37_chr13:48809655-48810242 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr13:48815437-48815937 Neighboring gene integral membrane protein 2B

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. Genotype-phenotype correlations in patients with retinoblastoma and interstitial 13q deletions. Mitter D, et al. Eur J Hum Genet, 2011 Sep. PMID 21505449, Free PMC Article
    2. The mediator of RNA polymerase II. Blazek E, et al. Chromosoma, 2005 Mar. PMID 15690163
    3. The mammalian Mediator complex. Conaway JW, et al. FEBS Lett, 2005 Feb 7. PMID 15680972
    4. The survival gene MED4 explains low penetrance retinoblastoma in patients with large RB1 deletion. Dehainault C, et al. Hum Mol Genet, 2014 Oct 1. PMID 24858910
    5. Gene dosage, expression, and ontology analysis identifies driver genes in the carcinogenesis and chemoradioresistance of cervical cancer. Lando M, et al. PLoS Genet, 2009 Nov. PMID 19911042, Free PMC Article

    See all (104) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    Genotypes

    HIV-1 interactions

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    Replication interactions

    Interaction Pubs
    Knockdown of mediator complex subunit 4 (MED4) by siRNA inhibits HIV-1 replication in HeLa-derived TZM-bl cells PubMed
    Knockdown of mediator complex subunit 4 (MED4) by siRNA inhibits HIV-1 replication in HeLa P4/R5 cells PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Tat tat Knockdown of MED4 by siRNAs inhibits HIV LTR-beta-gal activation in the Tat transactivation assay PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Clone Names

    • FLJ10956

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables nuclear receptor coactivator activity NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    enables nuclear thyroid hormone receptor binding IDA
    Inferred from Direct Assay
    more info
    		  
    enables nuclear vitamin D receptor binding NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transcription coactivator activity IDA
    Inferred from Direct Assay
    more info
    		  
    enables transcription coregulator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables transcription coregulator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in RNA polymerase II preinitiation complex assembly NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in positive regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		  
    involved_in positive regulation of transcription elongation by RNA polymerase II NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in positive regulation of transcription initiation by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    		  
    involved_in positive regulation of transcription initiation by RNA polymerase II NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in regulation of transcription by RNA polymerase II IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in transcription by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of core mediator complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of core mediator complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of mediator complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane HDA PubMed 
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
    		  
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    mediator of RNA polymerase II transcription subunit 4
    Names
    activator-recruited cofactor 36 kDa component
    mediator, 34-kD subunit, homolog
    vitamin D receptor-interacting protein, 36-kD
    vitamin D3 receptor-interacting protein complex 36 kDa component

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001270629.2 → NP_001257558.1  mediator of RNA polymerase II transcription subunit 4 isoform 2

      See identical proteins and their annotated locations for NP_001257558.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) uses an alternate 5' exon structure and initiates translation at a downstream in-frame start codon compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1.
      Source sequence(s)
      AI613470, AK301835, AL158196
      Consensus CDS
      CCDS59241.1
      UniProtKB/TrEMBL
      Q5T911
      Related
      ENSP00000367849.1, ENST00000378586.5
      Conserved Domains (1) summary
      pfam10018
      Location:18 → 143
      Med4; Vitamin-D-receptor interacting Mediator subunit 4

    2. NM_014166.4 → NP_054885.1  mediator of RNA polymerase II transcription subunit 4 isoform 1

      See identical proteins and their annotated locations for NP_054885.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
      Source sequence(s)
      AI613470, AK001934, AL158196
      Consensus CDS
      CCDS9408.1
      UniProtKB/Swiss-Prot
      B4DX67, Q53GB4, Q53H68, Q5T912, Q6FHC4, Q6IA79, Q9BS95, Q9NPJ6, Q9NYR5
      UniProtKB/TrEMBL
      U4PSC4
      Related
      ENSP00000258648.2, ENST00000258648.7
      Conserved Domains (1) summary
      pfam10018
      Location:64 → 189
      Med4; Vitamin-D-receptor interacting Mediator subunit 4

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000013.11 Reference GRCh38.p14 Primary Assembly

      Range
      48075724..48095104 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060937.1 Alternate T2T-CHM13v2.0

      Range
      47296104..47315464 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9NPJ6.1 GenPept UniProtKB/Swiss-Prot:Q9NPJ6

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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