Git1 GIT ArfGAP 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Git1provided by MGI
Official Full Name: GIT ArfGAP 1provided by MGI
Primary source: MGI:MGI:1927140
See related: Ensembl:ENSMUSG00000011877 Vega:OTTMUSG00000008178
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Cat-1; p95Cat
Expression: Broad expression in testis adult (RPKM 225.3), cortex adult (RPKM 49.2) and 23 other tissues See more
Orthologs: human all

Genomic context

Location:: 11; 11 B5

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
106	current	GRCm38.p4 (GCF_000001635.24)	11	NC_000077.6 (77493412..77507775)
Build 37.2	previous assembly	MGSCv37 (GCF_000001635.18)	11	NC_000077.5 (77306914..77321276)

Chromosome 11 - NC_000077.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

Go to the top of the page Help

See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

Results provide evidence that loss of GIT1 alone is insufficient to drive a robust ADHD phenotype in distinct strains of mice. In contrast, multiple learning and memory defects have been observed using distinct GIT1-knockout lines, consistent with a predominant intellectual disability phenotype related to altered synaptic structural plasticity.
Title: GIT1 regulates synaptic structural plasticity underlying learning.
GIT1 gene deletion can inhibit chondrocyte proliferation and apoptosis during the recovery of tibial plateau fracture, so as to delay chondrocyte differentiation and tibial plateau fracture healing.
Title: GIT1 gene deletion delays chondrocyte differentiation and healing of tibial plateau fracture through suppressing proliferation and apoptosis of chondrocyte.
In astrocytes and neural stem cells, we identified G-protein-coupled receptor kinase-interacting protein 1 (GIT1) as a novel mTOR-binding protein, creating a unique mTOR complex lacking Raptor and Rictor
Title: Proteomic analysis reveals GIT1 as a novel mTOR complex component critical for mediating astrocyte survival.
studies indicated that GIT1 promotes metastasis of NSCLC cells. GIT1 was found to stimulate migration/invasion by altering the activity of Rac1/Cdc42 in NSCLC cells.
Title: GIT1 promotes lung cancer cell metastasis through modulating Rac1/Cdc42 activity and is associated with poor prognosis.
This study demonstrated that the Presynaptic Deletion of GIT Proteins Results in Increased Synaptic Strength at a Mammalian Central Synapse.
Title: Presynaptic Deletion of GIT Proteins Results in Increased Synaptic Strength at a Mammalian Central Synapse.
Results suggested that cyclic phosphorylation-dephosphorylation at Tyr- 554 of Git1 was crucial for dynamic interactions between Git1 and paxillin/Hic-5 in order to ensure coordinated cell motility.
Title: Specific dephosphorylation at tyr-554 of git1 by ptprz promotes its association with paxillin and hic-5.
It provides for the first time, to our knowledge, a possible mechanism for the concerted action of tyrosine kinases, GIT1/betaPIX proteins, and Ca(2+) in the propagation of signals leading to the regulation of microtubule nucleation in activated mast cells.
Title: Microtubule nucleation in mouse bone marrow-derived mast cells is regulated by the concerted action of GIT1/βPIX proteins and calcium.
results indicate that GIT1 regulates Smad1/5/8 phosphorylation and mediates BMP2 regulation of Runx2 expression, thus affecting endochondral ossification at the fracture site.
Title: Decreased BMP2 signal in GIT1 knockout mice slows bone healing.
Plateletderived growth factor inhibits osteoblast apoptosis through upregulating GIT1 expression.
Title: Platelet‑derived growth factor promotes osteoblast proliferation by activating G‑protein‑coupled receptor kinase interactor‑1.
GIT1 is an important signaling participant in fracture healing, with gene ablation leading to reduced callus vascularity and reduced osteoclast number in the healing callus.
Title: Impaired angiogenesis during fracture healing in GPCR kinase 2 interacting protein-1 (GIT1) knock out mice.

Submit: New GeneRIF Correction See all GeneRIFs (26)

Variation

Go to the top of the page Help

Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1) 1 citation
Targeted (4) 1 citation

Pathways from BioSystems

Go to the top of the page Help

Axon guidance, organism-specific biosystem (from REACTOME)
Axon guidance, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Developmental Biology, organism-specific biosystem (from REACTOME)
Developmental Biology, organism-specific biosystemcomputationally inferred pathway (not manually curated)
EGFR1 Signaling Pathway, organism-specific biosystem (from WikiPathways)
EGFR1 Signaling Pathway, organism-specific biosystemThe androgen receptor is a member of the nuclear receptor family of ligand activated transcription factors. These receptors bind to steroid hormones, thyroid hormone, retinoids and vitamin D among ot...
EPH-Ephrin signaling, organism-specific biosystem (from REACTOME)
EPH-Ephrin signaling, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Endocytosis, organism-specific biosystem (from KEGG)
Endocytosis, organism-specific biosystemEndocytosis is a mechanism for cells to remove ligands, nutrients, and plasma membrane (PM) proteins, and lipids from the cell surface, bringing them into the cell interior. Transmembrane proteins en...
Endocytosis, conserved biosystem (from KEGG)
Endocytosis, conserved biosystemEndocytosis is a mechanism for cells to remove ligands, nutrients, and plasma membrane (PM) proteins, and lipids from the cell surface, bringing them into the cell interior. Transmembrane proteins en...
Ephrin signaling, organism-specific biosystem (from REACTOME)
Ephrin signaling, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Regulation of Actin Cytoskeleton, organism-specific biosystem (from WikiPathways)
Regulation of Actin Cytoskeleton, organism-specific biosystem
Regulation of Actin Cytoskeleton
Regulation of actin cytoskeleton, organism-specific biosystem (from KEGG)
Regulation of actin cytoskeleton, organism-specific biosystem
Regulation of actin cytoskeleton
Regulation of actin cytoskeleton, conserved biosystem (from KEGG)
Regulation of actin cytoskeleton, conserved biosystem
Regulation of actin cytoskeleton
XPodNet - protein-protein interactions in the podocyte expanded by STRING, organism-specific biosystem (from WikiPathways)
XPodNet - protein-protein interactions in the podocyte expanded by STRING, organism-specific biosystemXPodNet is a larger version of PodNet. Interactions from STRING database have been added to PodNet to build XPodNet. Hence, not all interactions found in XPodNet do necessarily have to be curated.

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Homology

Homologs of the Git1 gene: The Git1 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, rat, chicken, and zebrafish.
Orthologs from Annotation Pipeline: 211 organisms have orthologs with human gene Git1
The Hierarchical Catalog of Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
GTPase activator activity	ISO Inferred from Sequence Orthology more info
metal ion binding	IEA Inferred from Electronic Annotation more info
protein binding	IPI Inferred from Physical Interaction more info	PubMed
protein homodimerization activity	ISO Inferred from Sequence Orthology more info
protein-containing complex binding	IPI Inferred from Physical Interaction more info	PubMed
small GTPase binding	ISO Inferred from Sequence Orthology more info
structural constituent of postsynaptic specialization	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
cellular response to epidermal growth factor stimulus	ISO Inferred from Sequence Orthology more info
dendritic spine development	ISO Inferred from Sequence Orthology more info	PubMed
ephrin receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
immunological synapse formation	ISO Inferred from Sequence Orthology more info	PubMed
negative regulation of ARF protein signal transduction	ISO Inferred from Sequence Orthology more info
neurotransmitter receptor localization to postsynaptic specialization membrane	ISO Inferred from Sequence Orthology more info
positive regulation of receptor catabolic process	ISO Inferred from Sequence Orthology more info
presynaptic modulation of chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
regulation of G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
regulation of cytokinesis	ISO Inferred from Sequence Orthology more info
regulation of synaptic vesicle exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
GABA-ergic synapse	ISO Inferred from Sequence Orthology more info
calyx of Held	IMP Inferred from Mutant Phenotype more info	PubMed
cytoplasm	IEA Inferred from Electronic Annotation more info
cytosol	ISO Inferred from Sequence Orthology more info
endosome	ISO Inferred from Sequence Orthology more info
focal adhesion	ISO Inferred from Sequence Orthology more info
glutamatergic synapse	ISO Inferred from Sequence Orthology more info
neuron projection	ISO Inferred from Sequence Orthology more info
postsynapse	ISO Inferred from Sequence Orthology more info
synapse	ISO Inferred from Sequence Orthology more info

General protein information

Go to the top of the page Help

Preferred Names: ARF GTPase-activating protein GIT1

Names: ARF GAP GIT1; G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein; G protein-coupled receptor kinase-interactor 1; GRK-interacting protein 1

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001004144.1 → NP_001004144.1 ARF GTPase-activating protein GIT1

See identical proteins and their annotated locations for NP_001004144.1

Status: PROVISIONAL

Source sequence(s)

BC079870

Consensus CDS

CCDS25080.1

UniProtKB/Swiss-Prot

Q68FF6

Related

ENSMUSP00000037210.3, OTTMUSP00000008532, ENSMUST00000037285.9, OTTMUST00000018574

Conserved Domains (7) summary

smart00105
Location:3 → 124

ArfGap; Putative GTP-ase activating proteins for the small GTPase, ARF

cd00204
Location:123 → 220

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam08518
Location:337 → 365

GIT_SHD; Spa2 homology domain (SHD) of GIT

pfam12205
Location:649 → 763

GIT1_C; G protein-coupled receptor kinase-interacting protein 1 C term

pfam12796
Location:137 → 229

Ank_2; Ankyrin repeats (3 copies)

pfam16559
Location:418 → 482

GIT_CC; GIT coiled-coil Rho guanine nucleotide exchange factor

sd00045
Location:137 → 165

ANK; ANK repeat [structural motif]

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 106

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm38.p4 C57BL/6J

Genomic

NC_000077.6 Reference GRCm38.p4 C57BL/6J

Range

77493412..77507775

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006532949.2 → XP_006533012.1 ARF GTPase-activating protein GIT1 isoform X1

UniProtKB/TrEMBL

Q5F258

Related

ENSMUSP00000098375.3, OTTMUSP00000008533, ENSMUST00000100812.3, OTTMUST00000018577

Conserved Domains (7) summary

smart00105
Location:3 → 124

ArfGap; Putative GTP-ase activating proteins for the small GTPase, ARF

cd00204
Location:123 → 220

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam08518
Location:328 → 356

GIT_SHD; Spa2 homology domain (SHD) of GIT

pfam12205
Location:640 → 754

GIT1_C; G protein-coupled receptor kinase-interacting protein 1 C term

pfam12796
Location:137 → 229

Ank_2; Ankyrin repeats (3 copies)

pfam16559
Location:409 → 473

GIT_CC; GIT coiled-coil Rho guanine nucleotide exchange factor

sd00045
Location:137 → 165

ANK; ANK repeat [structural motif]