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    rrf-3 RNA-directed RNA polymerase [ Caenorhabditis elegans ]

    Gene ID: 174315, updated on 9-Jun-2025
    Official Symbol
    rrf-3
    Official Full Name
    RNA-directed RNA polymerase
    Primary source
    WormBase:WBGene00004510
    Locus tag
    CELE_F10B5.7
    See related
    AllianceGenome:WB:WBGene00004510
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Caenorhabditis elegans (strain: Bristol N2)
    Lineage
    Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
    Summary
    Predicted to enable RNA-directed RNA polymerase activity. Involved in regulatory ncRNA-mediated heterochromatin formation and spermatogenesis. Predicted to be part of nuclear RNA-directed RNA polymerase complex. Is expressed in excretory cell; head; intestine; nervous system; and tail. [provided by Alliance of Genome Resources, Jun 2025]
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    Location:
    chromosome: II
    Exon count:
    15
    Sequence:
    Chromosome: II; NC_003280.10 (8162465..8168651)

    Chromosome II - NC_003280.10Genomic Context describing neighboring genes Neighboring gene Transmembrane protein 138 Neighboring gene Putative pachytene checkpoint protein 2 Neighboring gene Cell division cycle protein 16 homolog Neighboring gene Uncharacterized protein Neighboring gene Venom protein Neighboring gene putative 2,4-dienoyl-CoA reductase 3 [(3E)-enoyl-CoA-producing]

    Genomic Sequence:
    NC_003280.10

    Go to nucleotide: Graphics FASTA GenBank

    Related articles in PubMed

    1. Selective sensitivity of Caenorhabditis elegans neurons to RNA interference. Asikainen S, et al. Neuroreport, 2005 Dec 19. PMID 16317341
    2. C. elegans aversive olfactory learning generates diverse intergenerational effects. Pereira AG, et al. J Neurogenet, 2020 Sep-Dec. PMID 32940103, Free PMC Article
    3. Highly efficient RNAi and Cas9-based auto-cloning systems for C. elegans research. Sturm Á, et al. Nucleic Acids Res, 2018 Sep 28. PMID 29924347, Free PMC Article
    4. Functional genomic approaches using the nematode Caenorhabditis elegans as a model system. Lee J, et al. J Biochem Mol Biol, 2004 Jan 31. PMID 14761308
    5. RNAi as a tool to study cell biology: building the genome-phenome bridge. Gunsalus KC, et al. Curr Opin Cell Biol, 2005 Feb. PMID 15661512

    See all (152) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. C. elegans aversive olfactory learning generates diverse intergenerational effects.
      Title: C. elegans aversive olfactory learning generates diverse intergenerational effects.
    2. Results show that the tandem tudor protein ERI-5 potentiates ERI endo-RNAi by tethering an RNA-dependent RNA polymerase (RdRP, F10B5.7) module to DCR-1.
      Title: Tudor domain ERI-5 tethers an RNA-dependent RNA polymerase to DCR-1 to potentiate endo-RNAi.
    3. Present evidence for successive engagement of two different RdRPs (RRF-3/RRF-1) in an endogenous siRNA-based mechanism targeting specific mRNAs in C. elegans soma.
      Title: Distinct phases of siRNA synthesis in an endogenous RNAi pathway in C. elegans soma.
    4. the function of Caenorhabditis elegans RRF-3 during spermatogenesis
      Title: A Caenorhabditis elegans RNA-directed RNA polymerase in sperm development and endogenous RNA interference.
    5. These results provide additional support for the hypothesis that RRF-3 and ERI-1 act together in the endo-siRNA pathway, and furthermore suggests their involvement in additional biological processes.
      Title: Whole genome microarray analysis of C. elegans rrf-3 and eri-1 mutants.
    6. loss of this makes C. elegans hypersensitive to RNAi.
      Title: Loss of the putative RNA-directed RNA polymerase RRF-3 makes C. elegans hypersensitive to RNAi.
    Submit: New GeneRIF Correction
    Products Interactant Other Gene Complex Source Pubs Description

    Markers

    Homology

    Gene Ontology Provided by WormBase

    Function Evidence Code Pubs
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA-directed RNA polymerase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables RNA-directed RNA polymerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in RNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of gene expression IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulatory ncRNA-mediated heterochromatin formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in siRNA processing IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in spermatogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of nuclear RNA-directed RNA polymerase complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Preferred Names
    RNA-directed RNA polymerase
    NP_495713.2
    • Confirmed by transcript evidence

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003280.10 Reference assembly

      Range
      8162465..8168651
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_063312.7 → NP_495713.2  RNA-directed RNA polymerase [Caenorhabditis elegans]

      See identical proteins and their annotated locations for NP_495713.2

      Status: REVIEWED

      UniProtKB/TrEMBL
      G5EE53
      Conserved Domains (1) summary
      pfam05183
      Location:692 → 1221
      RdRP; RNA dependent RNA polymerase
    Nucleotide Protein
    Heading Accession and Version

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