Mdm2 transformed mouse 3T3 cell double minute 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mdm2provided by MGI
Official Full Name: transformed mouse 3T3 cell double minute 2provided by MGI
Primary source: MGI:MGI:96952
See related: Ensembl:ENSMUSG00000020184 AllianceGenome:MGI:96952
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Mdm-2; 1700007J15Rik
Summary: Enables p53 binding activity and ubiquitin protein ligase activity. Involved in apoptotic process and positive regulation of muscle cell differentiation. Acts upstream of or within several processes, including blood vessel remodeling; circulatory system development; and traversing start control point of mitotic cell cycle. Located in cytoplasm and nucleolus. Is expressed in several structures, including alimentary system; embryo ectoderm; genitourinary system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in chronic myeloid leukemia; endocrine gland cancer (multiple); glioblastoma; ichthyosis; and sarcoma. Orthologous to human MDM2 (MDM2 proto-oncogene). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in testis adult (RPKM 10.8), CNS E11.5 (RPKM 9.8) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 10 D2; 10 66.32 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2023_04	current	GRCm39 (GCF_000001635.27)	10	NC_000076.7 (117524780..117546663, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	10	NC_000076.6 (117688875..117710758, complement)

Chromosome 10 - NC_000076.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

LncRNA NRON promotes tumorigenesis by enhancing MDM2 activity toward tumor suppressor substrates.
Title: LncRNA NRON promotes tumorigenesis by enhancing MDM2 activity toward tumor suppressor substrates.
Coupled deglycosylation-ubiquitination cascade in regulating PD-1 degradation by MDM2.
Title: Coupled deglycosylation-ubiquitination cascade in regulating PD-1 degradation by MDM2.
MDM2 inhibition is synthetic lethal with PTEN loss in colorectal cancer cells via the p53-dependent mechanism.
Title: MDM2 inhibition is synthetic lethal with PTEN loss in colorectal cancer cells via the p53-dependent mechanism.
MDM2 Inhibition Enhances Immune Checkpoint Inhibitor Efficacy by Increasing IL15 and MHC Class II Production.
Title: MDM2 Inhibition Enhances Immune Checkpoint Inhibitor Efficacy by Increasing IL15 and MHC Class II Production.
Mdm2/p53 levels in bone marrow mesenchymal stromal cells are essential for maintaining the hematopoietic niche in response to DNA damage.
Title: Mdm2/p53 levels in bone marrow mesenchymal stromal cells are essential for maintaining the hematopoietic niche in response to DNA damage.
Alterations of the Mdm2 C-Terminus Differentially Impact Its Function In Vivo.
Title: Alterations of the Mdm2 C-Terminus Differentially Impact Its Function In Vivo.
Dlx3 Ubiquitination by Nuclear Mdm2 Is Essential for Dentinogenesis in Mice.
Title: Dlx3 Ubiquitination by Nuclear Mdm2 Is Essential for Dentinogenesis in Mice.
Preliminary evaluation of a small interfering RNA molecular probe targeting murine double minute 2 in breast cancer.
Title: Preliminary evaluation of a small interfering RNA molecular probe targeting murine double minute 2 in breast cancer.
Hepatic MDM2 Causes Metabolic Associated Fatty Liver Disease by Blocking Triglyceride-VLDL Secretion via ApoB Degradation.
Title: Hepatic MDM2 Causes Metabolic Associated Fatty Liver Disease by Blocking Triglyceride-VLDL Secretion via ApoB Degradation.
MDM2 E3 ligase activity is essential for p53 regulation and cell cycle integrity.
Title: MDM2 E3 ligase activity is essential for p53 regulation and cell cycle integrity.

Submit: New GeneRIF Correction See all GeneRIFs (239)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1) 1 citation
Targeted (24) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH124271 (e-PCR)
- UniSTS:161093

D0S2246 (e-PCR)
- UniSTS:147572

NoName (e-PCR)
- UniSTS:236466

Mdm2 (e-PCR), detects polymorphism
- UniSTS:143848

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables 5S rRNA binding	ISO Inferred from Sequence Orthology more info
enables NEDD8 ligase activity	ISO Inferred from Sequence Orthology more info
enables SUMO transferase activity	ISO Inferred from Sequence Orthology more info
enables disordered domain specific binding	ISO Inferred from Sequence Orthology more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables ligase activity	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables p53 binding	IBA Inferred from Biological aspect of Ancestor more info	PubMed
enables p53 binding	IPI Inferred from Physical Interaction more info	PubMed
enables p53 binding	ISO Inferred from Sequence Orthology more info
enables peroxisome proliferator activated receptor binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info
enables receptor serine/threonine kinase binding	ISO Inferred from Sequence Orthology more info
enables ribonucleoprotein complex binding	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed
enables ubiquitin protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest	ISO Inferred from Sequence Orthology more info
involved_in amyloid fibril formation	ISO Inferred from Sequence Orthology more info
involved_in apoptotic process	IDA Inferred from Direct Assay more info	PubMed
involved_in apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within atrial septum development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within atrioventricular valve morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within blood vessel development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within blood vessel remodeling	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cardiac septum morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cell cycle	IBA Inferred from Biological aspect of Ancestor more info	PubMed
acts_upstream_of cell cycle	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to actinomycin D	ISO Inferred from Sequence Orthology more info
involved_in cellular response to gamma radiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within endocardial cushion morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within endocardial cushion morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of protein localization	ISO Inferred from Sequence Orthology more info
involved_in fibroblast activation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within heart development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within heart development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within heart valve development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of DNA damage response, signal transduction by p53 class mediator	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cysteine-type endopeptidase activity involved in apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of neuron projection development	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of protein processing	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of signal transduction by p53 class mediator	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of cell cycle	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of mitotic cell cycle	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of muscle cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein export from nucleus	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of vascular associated smooth muscle cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of vascular associated smooth muscle cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in protein autoubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein destabilization	ISO Inferred from Sequence Orthology more info
involved_in protein localization to nucleus	ISO Inferred from Sequence Orthology more info
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in protein ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein-containing complex assembly	ISO Inferred from Sequence Orthology more info
involved_in proteolysis involved in protein catabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of regulation of cell cycle	ISO Inferred from Sequence Orthology more info
involved_in regulation of gene expression	IBA Inferred from Biological aspect of Ancestor more info	PubMed
acts_upstream_of_or_within regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of heart rate	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in response to water-immersion restraint stress	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within traversing start control point of mitotic cell cycle	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within ventricular septum development	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	TAS Traceable Author Statement more info
colocalizes_with nuclear body	ISO Inferred from Sequence Orthology more info
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info
part_of transcription repressor complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase Mdm2

Names: RING-type E3 ubiquitin transferase Mdm2; double minute 2 protein; oncoprotein Mdm2; p53-binding protein Mdm2

NP_001275515.1

EC 2.3.2.27

NP_034916.1

EC 2.3.2.27

XP_017169311.1

EC 2.3.2.27

XP_036011554.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001288586.2 → NP_001275515.1 E3 ubiquitin-protein ligase Mdm2 isoform 2

See identical proteins and their annotated locations for NP_001275515.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an internal exon in the 5' region, which results in translation initiation at a downstream AUG start codon, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1.

Source sequence(s)

BC050902, BP767471, BY323121, CN703560

Consensus CDS

CCDS70110.1

UniProtKB/Swiss-Prot

P23804

UniProtKB/TrEMBL

Q3TVL4

Related

ENSMUSP00000100898.2, ENSMUST00000105263.9

Conserved Domains (3) summary

cd16783
Location:384 → 439

mRING-HC-C2H2C4_MDM2; Modified RING finger, HC subclass (C2H2C4-type), found in E3 ubiquitin-protein ligase MDM2 and similar proteins

pfam00641
Location:248 → 277

zf-RanBP; Zn-finger in Ran binding protein and others

cl38907
Location:1 → 58

SWIB-MDM2; SWIB/MDM2 domain family
NM_010786.4 → NP_034916.1 E3 ubiquitin-protein ligase Mdm2 isoform 1

See identical proteins and their annotated locations for NP_034916.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1).

Source sequence(s)

AK004719, BP767471, BY323121

Consensus CDS

CCDS24194.1

UniProtKB/Swiss-Prot

P23804, Q61040, Q64330, Q91XK7

UniProtKB/TrEMBL

Q3TVL4

Related

ENSMUSP00000020408.9, ENSMUST00000020408.16

Conserved Domains (3) summary

pfam00641
Location:297 → 326

zf-RanBP; Zn-finger in Ran binding protein and others

pfam02201
Location:46 → 92

SWIB; SWIB/MDM2 domain

pfam13920
Location:434 → 481

zf-C3HC4_3; Zinc finger, C3HC4 type (RING finger)

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2023_04

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000076.7 Reference GRCm39 C57BL/6J

Range

117524780..117546663 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036155661.1 → XP_036011554.1 E3 ubiquitin-protein ligase Mdm2 isoform X1

UniProtKB/TrEMBL

Q3TVL4

Conserved Domains (3) summary

cd16783
Location:384 → 439

mRING-HC-C2H2C4_MDM2; Modified RING finger, HC subclass (C2H2C4-type), found in E3 ubiquitin-protein ligase MDM2 and similar proteins

pfam00641
Location:248 → 277

zf-RanBP; Zn-finger in Ran binding protein and others

cl38907
Location:1 → 58

SWIB-MDM2; SWIB/MDM2 domain family
XM_017313822.2 → XP_017169311.1 E3 ubiquitin-protein ligase Mdm2 isoform X2

Conserved Domains (2) summary

cd16783
Location:236 → 291

mRING-HC-C2H2C4_MDM2; Modified RING finger, HC subclass (C2H2C4-type), found in E3 ubiquitin-protein ligase MDM2 and similar proteins

pfam00641
Location:101 → 129

zf-RanBP; Zn-finger in Ran binding protein and others