Mapre1 microtubule-associated protein, RP/EB family, member 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mapre1provided by MGI
Official Full Name: microtubule-associated protein, RP/EB family, member 1provided by MGI
Primary source: MGI:MGI:891995
See related: Ensembl:ENSMUSG00000027479 AllianceGenome:MGI:891995
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Eb1; BIM1p; D2Ertd459e; 5530600P05Rik
Summary: Enables microtubule plus-end binding activity. Involved in microtubule bundle formation and microtubule polymerization. Acts upstream of or within non-motile cilium assembly; positive regulation of microtubule plus-end binding activity; and protein localization to microtubule cytoskeleton. Located in Golgi apparatus; cell projection membrane; and microtubule cytoskeleton. Is expressed in several structures, including alimentary system; brain; genitourinary system; hemolymphoid system gland; and liver and biliary system. Orthologous to human MAPRE1 (microtubule associated protein RP/EB family member 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in CNS E18 (RPKM 22.9), CNS E11.5 (RPKM 21.4) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 H1; 2 75.95 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
109	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (153583131..153615237)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (153741287..153773317)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Targeting the Microtubule EB1-CLASP2 Complex Modulates NaV1.5 at Intercalated Discs.
Title: Targeting the Microtubule EB1-CLASP2 Complex Modulates Na<sub>V</sub>1.5 at Intercalated Discs.
MACF1 promotes preosteoblast migration by mediating focal adhesion turnover through EB1.
Title: MACF1 promotes preosteoblast migration by mediating focal adhesion turnover through EB1.
Arl4D-EB1 interaction promotes centrosomal recruitment of EB1 and microtubule growth.
Title: Arl4D-EB1 interaction promotes centrosomal recruitment of EB1 and microtubule growth.
Crosstalk between EB1 protein and Ankyrin-G is decisive for axon initial segment assembly.
Title: Cooperative Interactions between 480 kDa Ankyrin-G and EB Proteins Assemble the Axon Initial Segment.
We describe here a novel function for tau as a direct regulator of eb1 and eb2 proteins in differentiating neuronal cells
Title: Tau regulates the localization and function of End-binding proteins 1 and 3 in developing neuronal cells.
Data indicate that cortactin as an Src-dependent interacting partner of EB1.
Title: Microtubule dynamic instability controls podosome patterning in osteoclasts through EB1, cortactin, and Src.
Data indicate that EB1(MAPRE1) is up-regulated in osteoblasts and is required for bone cell differentiation.
Title: EB1 levels are elevated in ascorbic Acid (AA)-stimulated osteoblasts and mediate cell-cell adhesion-induced osteoblast differentiation.
MAP1B interacts directly with EB1 and EB3 (EBs), two core 'microtubule plus-end tracking proteins' ( TIPs), and sequesters them in the cytosol of developing neuronal cells.
Title: MAP1B regulates microtubule dynamics by sequestering EB1/3 in the cytosol of developing neuronal cells.
the Amer2-EB1-APC complex regulates cell migration by altering microtubule stability.
Title: Amer2 protein interacts with EB1 protein and adenomatous polyposis coli (APC) and controls microtubule stability and cell migration.
Detyrosination regulates CAP-Gly proteins interaction with alpha-tubulin, but not with EB1.
Title: Cap-Gly proteins at microtubule plus ends: is EB1 detyrosination involved?

Submit: New GeneRIF Correction See all GeneRIFs (19)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AI462499 (e-PCR)
- UniSTS:178740

AW260097 (e-PCR)
- UniSTS:178741

Mapre1 (e-PCR), detects polymorphism
- UniSTS:527418

AI504412 (e-PCR)
- UniSTS:159054

Mapre1 (e-PCR), detects polymorphism
- UniSTS:142942

C86711 (e-PCR)
- UniSTS:167321

Homology

Homologs of the Mapre1 gene: The Mapre1 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, rat, chicken, zebrafish, S.cerevisiae, K.lactis, E.gossypii, S.pombe, M.oryzae, N.crassa, A.thaliana, rice, and frog.
Orthologs from Annotation Pipeline: 325 organisms have orthologs with human gene Mapre1
Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables microtubule binding	IEA Inferred from Electronic Annotation more info
enables microtubule plus-end binding	IBA Inferred from Biological aspect of Ancestor more info	PubMed
enables microtubule plus-end binding	IDA Inferred from Direct Assay more info	PubMed
enables microtubule plus-end binding	ISO Inferred from Sequence Orthology more info
enables molecular adaptor activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in attachment of mitotic spindle microtubules to kinetochore	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cell cycle	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell division	IEA Inferred from Electronic Annotation more info
involved_in cell migration	ISO Inferred from Sequence Orthology more info
involved_in establishment of mitotic spindle orientation	ISO Inferred from Sequence Orthology more info
involved_in microtubule bundle formation	IDA Inferred from Direct Assay more info	PubMed
involved_in microtubule polymerization	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of microtubule polymerization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within non-motile cilium assembly	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of microtubule plus-end binding	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of microtubule plus-end binding	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of microtubule polymerization	ISO Inferred from Sequence Orthology more info
involved_in protein localization to astral microtubule	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein localization to centrosome	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to microtubule	IBA Inferred from Biological aspect of Ancestor more info	PubMed
acts_upstream_of_or_within protein localization to microtubule	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to microtubule	ISO Inferred from Sequence Orthology more info
involved_in regulation of microtubule polymerization or depolymerization	IBA Inferred from Biological aspect of Ancestor more info	PubMed
involved_in spindle assembly	IBA Inferred from Biological aspect of Ancestor more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in cell projection	IDA Inferred from Direct Assay more info	PubMed
located_in cell projection membrane	IDA Inferred from Direct Assay more info	PubMed
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in ciliary basal body	IDA Inferred from Direct Assay more info	PubMed
located_in cortical microtubule cytoskeleton	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasmic microtubule	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in focal adhesion	ISO Inferred from Sequence Orthology more info
located_in microtubule	IDA Inferred from Direct Assay more info	PubMed
is_active_in microtubule	ISO Inferred from Sequence Orthology more info
located_in microtubule cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
is_active_in microtubule organizing center	IBA Inferred from Biological aspect of Ancestor more info	PubMed
is_active_in microtubule plus-end	IBA Inferred from Biological aspect of Ancestor more info	PubMed
located_in microtubule plus-end	IDA Inferred from Direct Assay more info	PubMed
located_in mitotic spindle astral microtubule end	ISO Inferred from Sequence Orthology more info
is_active_in mitotic spindle pole	ISO Inferred from Sequence Orthology more info
colocalizes_with spindle	ISO Inferred from Sequence Orthology more info
is_active_in spindle midzone	IBA Inferred from Biological aspect of Ancestor more info	PubMed

General protein information

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Preferred Names: microtubule-associated protein RP/EB family member 1

Names: APC-binding protein EB1; adenomatosis polyposis coli binding protein Eb1; end-binding protein 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_007896.3 → NP_031922.1 microtubule-associated protein RP/EB family member 1

See identical proteins and their annotated locations for NP_031922.1

Status: VALIDATED

Source sequence(s)

AL833803, BC043333

Consensus CDS

CCDS16917.1

UniProtKB/Swiss-Prot

Q61166, Q7TN34

UniProtKB/TrEMBL

A0A6P5NW00, A0A8C6HJV9, Q3U4H0

Related

ENSMUSP00000028981.9, ENSMUST00000028981.9

Conserved Domains (1) summary

COG5217
Location:16 → 257

BIM1; Microtubule-binding protein involved in cell cycle control [Cell division and chromosome partitioning / Cytoskeleton]

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 109 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000068.8 Reference GRCm39 C57BL/6J

Range

153583131..153615237

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036158139.1 → XP_036014032.1 microtubule-associated protein RP/EB family member 1 isoform X2

Conserved Domains (1) summary

COG5217
Location:16 → 160

BIM1; Microtubule-binding protein involved in cell cycle control [Cell division and chromosome partitioning / Cytoskeleton]
XM_036158136.1 → XP_036014029.1 microtubule-associated protein RP/EB family member 1 isoform X1

UniProtKB/Swiss-Prot

Q7TN34

UniProtKB/TrEMBL

A0A6P5NW00, A0A8C6HJV9, Q3U4H0

Conserved Domains (1) summary

COG5217
Location:16 → 257

BIM1; Microtubule-binding protein involved in cell cycle control [Cell division and chromosome partitioning / Cytoskeleton]