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    Dhfr dihydrofolate reductase [ Mus musculus (house mouse) ]

    Gene ID: 13361, updated on 9-May-2024

    Summary

    Official Symbol
    Dhfrprovided by MGI
    Official Full Name
    dihydrofolate reductaseprovided by MGI
    Primary source
    MGI:MGI:94890
    See related
    Ensembl:ENSMUSG00000021707 AllianceGenome:MGI:94890
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    8430436I03Rik
    Summary
    Enables dihydrofolate reductase activity. Involved in several processes, including positive regulation of nitric-oxide synthase activity; tetrahydrobiopterin biosynthetic process; and tetrahydrofolate interconversion. Located in mitochondrion and nucleus. Is expressed in several structures, including alimentary system; genitourinary system; hemolymphoid system; integumental system; and nervous system. Human ortholog(s) of this gene implicated in acute lymphoblastic leukemia. Orthologous to several human genes including DHFRP1 (dihydrofolate reductase pseudogene 1). [provided by Alliance of Genome Resources, Apr 2022]
    Expression
    Broad expression in liver E14 (RPKM 9.4), liver E14.5 (RPKM 7.0) and 22 other tissues See more
    Orthologs
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    Genomic context

    See Dhfr in Genome Data Viewer
    Location:
    13 C3; 13 47.64 cM
    Exon count:
    6
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 13 NC_000079.7 (92491291..92525561)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 13 NC_000079.6 (92354783..92389053)

    Chromosome 13 - NC_000079.7Genomic Context describing neighboring genes Neighboring gene RAS protein-specific guanine nucleotide-releasing factor 2 Neighboring gene predicted gene, 46435 Neighboring gene STARR-seq mESC enhancer starr_35260 Neighboring gene STARR-seq mESC enhancer starr_35261 Neighboring gene STARR-seq mESC enhancer starr_35262 Neighboring gene mutS homolog 3 Neighboring gene STARR-positive B cell enhancer mm9_chr13:93090941-93091242 Neighboring gene predicted gene, 20379 Neighboring gene STARR-positive B cell enhancer ABC_E8539 Neighboring gene STARR-positive B cell enhancer ABC_E2416 Neighboring gene 60S ribosomal protein L32 pseudogene Neighboring gene ankyrin repeat domain 34B Neighboring gene STARR-seq mESC enhancer starr_35266 Neighboring gene family with sequence similarity 151, member B

    Genomic regions, transcripts, and products

    Expression

    • Project title: Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    Variation

    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Chemically induced (ENU) (1) 
    • Endonuclease-mediated (2)  1 citation
    • Targeted (4) 

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables NADP binding IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables NADP binding ISO
    Inferred from Sequence Orthology
    more info
     
    enables NADPH binding ISO
    Inferred from Sequence Orthology
    more info
     
    enables RNA binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables dihydrofolate reductase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables dihydrofolate reductase activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables dihydrofolate reductase activity ISO
    Inferred from Sequence Orthology
    more info
     
    enables dihydrofolic acid binding ISO
    Inferred from Sequence Orthology
    more info
     
    enables folic acid binding ISO
    Inferred from Sequence Orthology
    more info
     
    enables mRNA binding ISO
    Inferred from Sequence Orthology
    more info
     
    enables mRNA regulatory element binding translation repressor activity ISO
    Inferred from Sequence Orthology
    more info
     
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
     
    enables sequence-specific mRNA binding ISO
    Inferred from Sequence Orthology
    more info
     
    Process Evidence Code Pubs
    involved_in axon regeneration ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in dihydrofolate metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in dihydrofolate metabolic process ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in folic acid metabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in folic acid metabolic process ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in glycine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
     
    acts_upstream_of_or_within one-carbon metabolic process IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in positive regulation of nitric-oxide synthase activity IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in regulation of removal of superoxide radicals IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    acts_upstream_of_or_within response to methotrexate IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in tetrahydrobiopterin biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in tetrahydrofolate biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in tetrahydrofolate biosynthetic process ISO
    Inferred from Sequence Orthology
    more info
     
    involved_in tetrahydrofolate interconversion IDA
    Inferred from Direct Assay
    more info
    PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in cytoplasm ISO
    Inferred from Sequence Orthology
    more info
     
    located_in cytosol TAS
    Traceable Author Statement
    more info
     
    located_in mitochondrial inner membrane ISO
    Inferred from Sequence Orthology
    more info
     
    located_in mitochondrial matrix ISO
    Inferred from Sequence Orthology
    more info
     
    is_active_in mitochondrion IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in mitochondrion IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in mitochondrion ISO
    Inferred from Sequence Orthology
    more info
     
    is_active_in nucleus IDA
    Inferred from Direct Assay
    more info
    PubMed 

    General protein information

    Preferred Names
    dihydrofolate reductase
    NP_034179.1

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_010049.3NP_034179.1  dihydrofolate reductase

      See identical proteins and their annotated locations for NP_034179.1

      Status: VALIDATED

      Source sequence(s)
      AI854085, AK031241, BU058996, CAAA01146114, CB247191
      Consensus CDS
      CCDS26680.1
      UniProtKB/Swiss-Prot
      P00375, P70693, Q61485, Q61487, Q61579
      UniProtKB/TrEMBL
      Q544T5
      Related
      ENSMUSP00000022218.5, ENSMUST00000022218.6
      Conserved Domains (1) summary
      cd00209
      Location:5184
      DHFR; Dihydrofolate reductase (DHFR). Reduces 7,8-dihydrofolate to 5,6,7,8-tetrahydrofolate with NADPH as a cofactor. This is an essential step in the biosynthesis of deoxythymidine phosphate since 5,6,7,8-tetrahydrofolate is required to regenerate 5, ...

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000079.7 Reference GRCm39 C57BL/6J

      Range
      92491291..92525561
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)