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    DDX17 DEAD-box helicase 17 [ Homo sapiens (human) ]

    Gene ID: 10521, updated on 7-Jul-2024

    Summary

    Official Symbol
    DDX17provided by HGNC
    Official Full Name
    DEAD-box helicase 17provided by HGNC
    Primary source
    HGNC:HGNC:2740
    See related
    Ensembl:ENSG00000100201 MIM:608469; AllianceGenome:HGNC:2740
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    P72; RH70
    Summary
    DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and splicesosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an ATPase activated by a variety of RNA species, but not by dsDNA. This protein, and that encoded by DDX5 gene, are more closely related to each other than to any other member of the DEAD box family. This gene can encode multiple isoforms due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) start codon. [provided by RefSeq, Apr 2011]
    Expression
    Ubiquitous expression in spleen (RPKM 144.6), endometrium (RPKM 136.4) and 25 other tissues See more
    Orthologs
    NEW
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    Genomic context

    See DDX17 in Genome Data Viewer
    Location:
    22q13.1
    Exon count:
    13
    Annotation release Status Assembly Chr Location
    RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 22 NC_000022.11 (38483438..38506311, complement)
    RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 22 NC_060946.1 (38947707..38970570, complement)
    105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 22 NC_000022.10 (38879443..38902316, complement)

    Chromosome 22 - NC_000022.11Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC105373029 Neighboring gene potassium inwardly rectifying channel subfamily J member 4 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr22:38839066-38839566 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr22:38847863-38848482 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr22:38848483-38849101 Neighboring gene uncharacterized LOC124905116 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr22:38857412-38857912 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr22:38859483-38859982 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr22:38861409-38861910 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 13719 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 13720 Neighboring gene KDEL endoplasmic reticulum protein retention receptor 3 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:38901381-38902272 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:38902273-38903164 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:38908748-38909556 Neighboring gene CRISPRi-validated cis-regulatory element chr22.1887 Neighboring gene DNA meiotic recombinase 1 Neighboring gene MPRA-validated peak4491 silencer Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 13723 Neighboring gene uncharacterized LOC105373031 Neighboring gene H3K27ac hESC enhancer GRCh37_chr22:38966777-38967287

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    HIV-1 interactions

    Replication interactions

    Interaction Pubs
    siRNA knockdown of DDX17 decreases intra- and extra-cellular HIV CA(p24) from HeLa cells transfected with env-deleted HIV-1 plasmid, a vesicular stomatitis virus glycoprotein plasmid and specific siRNA. Resulting HIV demonstrates decreased infectivity. PubMed
    siRNA knockdown of DDX17 reduced HIV-1 infectivity by 5 times and the extracelluar (supernatant) CA (p24) by a smiliar reduction without affecting the intracellular HIV-1 Gag levels PubMed
    Knockdown of DDX17 by siRNA or shRNA inhibits HIV-1 production in HeLa cells and infectivity in TZM-bl cells PubMed
    Knockdown of DDX17 by siRNA decreases the levels of total unspliced and spliced mRNAs, and results in reduction of HIV-1 production PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Envelope surface glycoprotein gp120 env Tandem affinity purification and mass spectrometry analysis identify DEAD (Asp-Glu-Ala-Asp) box helicase 17 (DDX17), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    Gag-Pol gag-pol Overexpression of DDX17 DQAD leads to a decrease in frameshift efficiency, suggesting that the helicase activity of DDX17 is involved in maintaining the proper ratio of Gag-Pol expression required for optimal infectivity PubMed
    gag-pol Tandem affinity purification and mass spectrometry analysis identify DEAD (Asp-Glu-Ala-Asp) box helicase 17 (DDX17), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    Nef nef Tandem affinity purification and mass spectrometry analysis identify DEAD (Asp-Glu-Ala-Asp) box helicase 17 (DDX17), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    Pr55(Gag) gag HIV-1 Gag interacts with DDX17 as demonstrated by proximity dependent biotinylation proteomics and validated via immunoprecipitation and western blot analysis PubMed
    gag siRNA knockdown of DDX17 DOES NOT affect intracellular Gag levels but does decrease extracellular (supernatant) CA (p24) levels upon HIV-1 infection PubMed
    gag Expression of DDX17 DQAD mutant decreases the amount of HIV-1 CA but not Gag, suggesting that the mutant inhibits Gag processing PubMed
    gag Tandem affinity purification and mass spectrometry analysis identify DEAD (Asp-Glu-Ala-Asp) box helicase 17 (DDX17), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
    Rev rev Coexpression of HIV-1 Rev with DDX17 greatly upregulate the expression of HIV-1 CA in HeLa cells PubMed
    rev DDX17 interacts with HIV-1 Rev and enhances the Rev function. DDX17 partially co-localizes with Rev in the nucleolus PubMed
    rev HIV-1 Rev interacting protein, DEAD (Asp-Glu-Ala-Asp) box polypeptide 17 (DDX17), is identified by the in-vitro binding experiments involving cytosolic or nuclear extracts from HeLa cells. The interaction of Rev with DDX17 is increased by RRE PubMed
    Tat tat DEAD (Asp-Glu-Ala-Asp) box helicase 17 (DDX17) is identified to interact with HIV-1 Tat mutant Nullbasic in HeLa cells by LC MS/MS PubMed
    capsid gag siRNA knockdown of DDX17 decreases intra- and extra-cellular HIV CA(p24) from HeLa cells transfected with env-deleted HIV-1 plasmid, a vesicular stomatitis virus glycoprotein plasmid and specific siRNA. Resulting HIV demonstrates decreased infectivity. PubMed
    gag siRNA knockdown of DDX17 decreases extracellular (supernatant) CA (p24) levels upon HIV-1 infection BUT DOES NOT affect intracellular Gag levels PubMed
    gag Coexpression of HIV-1 Rev with DDX17 greatly upregulate the expression of HIV-1 CA in HeLa cells PubMed
    gag Expression of DDX17 DQAD mutant decreases the amount of HIV-1 CA but not Gag, suggesting that the mutant inhibits Gag processing PubMed
    retropepsin gag-pol Positional proteomics analysis identifies the cleavage of human DEAD (Asp-Glu-Ala-Asp) box helicase 17 (DDX17) at amino acid residues 573-574 by the HIV-1 protease PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Clone Names

    • DKFZp761H2016

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
     
    enables ATP-dependent activity, acting on RNA TAS
    Traceable Author Statement
    more info
    PubMed 
    enables RNA binding HDA PubMed 
    enables RNA helicase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables mRNA 3'-UTR binding IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables ribonucleoprotein complex binding IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables transcription coactivator activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    Process Evidence Code Pubs
    involved_in RNA processing TAS
    Traceable Author Statement
    more info
    PubMed 
    involved_in alternative mRNA splicing, via spliceosome IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in alternative mRNA splicing, via spliceosome IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in androgen receptor signaling pathway IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in defense response to virus IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in epithelial to mesenchymal transition IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in estrogen receptor signaling pathway IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in immune system process IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in miRNA metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in myoblast differentiation ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in positive regulation of transcription by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in rRNA processing IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in regulation of alternative mRNA splicing, via spliceosome IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in regulation of skeletal muscle cell differentiation IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in regulation of transcription by RNA polymerase II IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in regulatory ncRNA-mediated gene silencing IEA
    Inferred from Electronic Annotation
    more info
     
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in cytosol IEA
    Inferred from Electronic Annotation
    more info
     
    located_in membrane HDA PubMed 
    located_in nuclear speck IDA
    Inferred from Direct Assay
    more info
     
    located_in nucleolus IEA
    Inferred from Electronic Annotation
    more info
     
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
     
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    part_of ribonucleoprotein complex IBA
    Inferred from Biological aspect of Ancestor
    more info
     

    General protein information

    Preferred Names
    probable ATP-dependent RNA helicase DDX17
    Names
    DEAD (Asp-Glu-Ala-Asp) box helicase 17
    DEAD (Asp-Glu-Ala-Asp) box polypeptide 17
    DEAD box protein p72
    DEAD box protein p82
    DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 17 (72kD)
    RNA-dependent helicase p72
    p72 RNA helicase
    NP_001091974.1
    NP_006377.2

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_029642.1 RefSeqGene

      Range
      5030..27903
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001098504.2NP_001091974.1  probable ATP-dependent RNA helicase DDX17 isoform 3 precursor

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) uses a different donor splice site (6 nt downstream) in the penultimate coding exon, but initiates translation from the same non-AUG (CUG) codon as transcript variant 1, resulting in an isoform (3) that is 2 aa longer than isoform 1. Alternate translation from an in-frame, downstream AUG yields a shorter isoform.
      Source sequence(s)
      AB209595, AK024985, AL080113, BP353248, CB215934, Z97056
      Consensus CDS
      CCDS46706.1
      UniProtKB/TrEMBL
      A0A1X7SBZ2, A0A5H1ZRQ2
      Related
      ENSP00000380033.4, ENST00000396821.8
      Conserved Domains (3) summary
      smart00487
      Location:191393
      DEXDc; DEAD-like helicases superfamily
      cd00079
      Location:389521
      HELICc; Helicase superfamily c-terminal domain; associated with DEXDc-, DEAD-, and DEAH-box proteins, yeast initiation factor 4A, Ski2p, and Hepatitis C virus NS3 helicases; this domain is found in a wide variety of helicases and helicase related proteins; may ...
      cd00268
      Location:173378
      DEADc; DEAD-box helicases. A diverse family of proteins involved in ATP-dependent RNA unwinding, needed in a variety of cellular processes including splicing, ribosome biogenesis and RNA degradation. The name derives from the sequence of the Walker B motif ...
    2. NM_006386.5NP_006377.2  probable ATP-dependent RNA helicase DDX17 isoform 1 precursor

      See identical proteins and their annotated locations for NP_006377.2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) encodes isoform 1 (also known as p82) through the use of a non-AUG (CUG) translation initiation codon. Alternate translation from an in-frame, downstream AUG results in a shorter isoform (known as p72).
      Source sequence(s)
      AK024985, AL080113, BP353248, CB215934, Z97056
      Consensus CDS
      CCDS33646.1
      UniProtKB/Swiss-Prot
      B1AHM0, H3BLZ8, Q69YT1, Q6ICD6, Q92841
      UniProtKB/TrEMBL
      A0A1X7SBZ2
      Related
      ENSP00000385536.2, ENST00000403230.3
      Conserved Domains (3) summary
      smart00487
      Location:191393
      DEXDc; DEAD-like helicases superfamily
      cd00079
      Location:389521
      HELICc; Helicase superfamily c-terminal domain; associated with DEXDc-, DEAD-, and DEAH-box proteins, yeast initiation factor 4A, Ski2p, and Hepatitis C virus NS3 helicases; this domain is found in a wide variety of helicases and helicase related proteins; may ...
      cd00268
      Location:173378
      DEADc; DEAD-box helicases. A diverse family of proteins involved in ATP-dependent RNA unwinding, needed in a variety of cellular processes including splicing, ribosome biogenesis and RNA degradation. The name derives from the sequence of the Walker B motif ...

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000022.11 Reference GRCh38.p14 Primary Assembly

      Range
      38483438..38506311 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060946.1 Alternate T2T-CHM13v2.0

      Range
      38947707..38970570 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Suppressed Reference Sequence(s)

    The following Reference Sequences have been suppressed. Explain

    1. NM_001098505.1: Suppressed sequence

      Description
      NM_001098505.1: This RefSeq was permanently suppressed because currently there is insufficient support for the transcript and the protein.
    2. NM_030881.3: Suppressed sequence

      Description
      NM_030881.3: This RefSeq was permanently suppressed because currently there is insufficient support for the transcript and the protein.