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sbcC ATP-dependent structure-specific DNA nuclease - SbcC subunit [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 949076, updated on 4-Feb-2026
Official Symbol
sbcC
Official Full Name
ATP-dependent structure-specific DNA nuclease - SbcC subunit
Primary source
ECOCYC:EG10927
Locus tag
b0397
See related
ASAP:ABE-0001381
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0391
Summary
SbcCD can cleave dsDNA 5' ends sealed by a hairpin or by covalent linko a protein. [More information is available at EcoGene: EG10927]. sbcC encodes a component of the SbcCD exonuclease . [More information is available at EcoCyc: EG10927].
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Sequence:
NC_000913.3 (412607..415753, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene fructokinase Neighboring gene putative transport protein AraJ Neighboring gene ATP-dependent structure-specific DNA nuclease - SbcD subunit Neighboring gene DNA-binding transcriptional dual regulator PhoB Neighboring gene sensor histidine kinase PhoR

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Linkage distortion following conjugational transfer of sbcC+ to recBC sbcBC strains of Escherichia coli. Lloyd RG. J Bacteriol, 1991 Sep. PMID 1885546, Free PMC Article
  2. Escherichia coli sbcC mutants permit stable propagation of DNA replicons containing a long palindrome. Chalker AF, et al. Gene, 1988 Nov 15. PMID 2975250
  3. Long DNA palindromes, cruciform structures, genetic instability and secondary structure repair. Leach DR. Bioessays, 1994 Dec. PMID 7840768
  4. DNA cleavage and degradation by the SbcCD protein complex from Escherichia coli. Connelly JC, et al. Nucleic Acids Res, 1999 Feb 15. PMID 9927737, Free PMC Article
  5. Evidence for two mechanisms of palindrome-stimulated deletion in Escherichia coli: single-strand annealing and replication slipped mispairing. Bzymek M, et al. Genetics, 2001 Jun. PMID 11404319, Free PMC Article

See all (46) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The E. coli Mre11-Rad50 homolog SbcC/SbcD can detect and process diverse DNA ends.
    Title: Mechanism of DNA End Sensing and Processing by the Mre11-Rad50 Complex.
  2. Data show that replication forks form a palindrome-like structural intermediate that requires nucleolytic processing by SbcC-SbcD and exonuclease I (ExoI) before chromosome replication can be completed.
    Title: SbcC-SbcD and ExoI process convergent forks to complete chromosome replication.
  3. The results suggest that SbcCD processes hairpin and linear dsDNA ends with a novel DNA end-dependent binary endonuclease activity in response to substrate length.
    Title: A novel mode of nuclease action is revealed by the bacterial Mre11/Rad50 complex.
  4. By initiating or participating in double-strand break repair, SbcCD may provide DNA substrates for Pol IV polymerase activity.
    Title: The SMC-like protein complex SbcCD enhances DNA polymerase IV-dependent spontaneous mutation in Escherichia coli.
  5. In sbcCD mutants, RecA4142 can bind other DNA substrates by itself that are normally degraded by the SbcCD nuclease.
    Title: RecA4142 causes SOS constitutive expression by loading onto reversed replication forks in Escherichia coli K-12.
  6. Study found that functions of SbcCD, a DNA structure specific nuclease, were needed in the RNase E-dependent lacZ expression of the GAA repeats.
    Title: The roles of SbcCD and RNaseE in the transcription of GAA x TTC repeats in Escherichia coli.
  7. The absence of SbcC and SbcD in sbcB15 cells decreased stationary-phase mutation.
    Title: Role of single-strand DNA 3'-5' exonuclease ExoI and nuclease SbcCD in stationary-phase mutation in Escherichia coli K-12.
  8. N-terminus verified by Edman degradation on complete protein
    Title: Overexpression, purification, and characterization of the SbcCD protein from Escherichia coli.
  9. Transcription of the sbcDC operon is shown to be dependent on starvation and RpoS protein, SbcC protein forms foci that colocalize with the replication factory.
    Title: SbcCD regulation and localization in Escherichia coli.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 3'-5'-DNA exonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA exonuclease activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA exonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables double-stranded DNA endonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables endonuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables exonuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables nuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables single-stranded DNA endodeoxyribonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA recombination IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA recombination NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in DNA repair IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within DNA repair IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in DNA repair NAS
Non-traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within DNA replication IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in DNA replication IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within DNA replication termination EXP
Inferred from Experiment
more info
 PubMed 
involved_in double-strand break repair IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of DNA repair complex IBA
Inferred from Biological aspect of Ancestor
more info
  
Preferred Names
ATP-dependent structure-specific DNA nuclease - SbcC subunit

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    412607..415753 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414931.1 ATP-dependent structure-specific DNA nuclease - SbcC subunit

    See identical proteins and their annotated locations for NP_414931.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A376FRL1
    Conserved Domains (1) summary
    PRK10246
    Location:1 → 1048
    PRK10246; exonuclease subunit SbcC; Provisional
Nucleotide Protein
Heading Accession and Version

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