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leuO DNA-binding transcriptional dual regulator LeuO [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 949034, updated on 23-Apr-2026
Official Symbol
leuO
Official Full Name
DNA-binding transcriptional dual regulator LeuO
Primary source
ECOCYC:EG10531
Locus tag
b0076
See related
ASAP:ABE-0000280
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0078
Summary
LysR-family. leuO is upstream and divergent of the leuABCD operon. [More information is available at EcoGene: EG10531]. LeuO is a dual transcriptional regulator that regulates genes involved in leucine biosynthesis , genes involved in the utilization of certain beta-glucosides , and genes encoding LuxR-type transcription factors . [More information is available at EcoCyc: EG10531].
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Sequence:
NC_000913.3 (84368..85312)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 3-isopropylmalate dehydrogenase Neighboring gene 2-isopropylmalate synthase Neighboring gene leu operon leader peptide Neighboring gene protein YabR Neighboring gene acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Characterization of the pleiotropic LysR-type transcription regulator LeuO of Escherichia coli. Fragel SM, et al. Nucleic Acids Res, 2019 Aug 22. PMID 31184713, Free PMC Article
  2. Cloning of an EcoRI-generated fragment of the leucine operon of Salmonella typhimurium. Hertzberg KM, et al. Gene, 1980 Jan. PMID 6987127
  3. ppGpp-dependent leuO expression in bacteria under stress. Fang M, et al. Biochem Biophys Res Commun, 2000 Sep 16. PMID 11006083
  4. LeuO expression in response to starvation for branched-chain amino acids. Majumder A, et al. J Biol Chem, 2001 Jun 1. PMID 11376008
  5. LeuO-mediated transcriptional derepression. Chen CC, et al. J Biol Chem, 2003 Sep 26. PMID 12871947

See all (30) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. DNA-binding transcriptional dual regulator LeuO (LeuO) is a tetramer that is formed by dimerization of the N-terminal DNA-binding domain (DBD) and C-terminal effector-binding domain (EBD).
    Title: Characterization of the pleiotropic LysR-type transcription regulator LeuO of Escherichia coli.
  2. BglJ-RcsB activates leuO, while LeuO inhibits activation by BglJ-RcsB. Furthermore, LeuO activates expression of bglJ, which is likewise H-NS repressed.
    Title: Correlation of Antagonistic Regulation of leuO Transcription with the Cellular Levels of BglJ-RcsB and LeuO in Escherichia coli.
  3. RcsB-BglJ binds to the leuO promoter region and activates one of two leuO promoters.
    Title: RcsB-BglJ activates the Escherichia coli leuO gene, encoding an H-NS antagonist and pleiotropic regulator of virulence determinants.
  4. LeuO is a major player in antagonistic interplay against the universal silencer H-NS.
    Title: Novel roles of LeuO in transcription regulation of E. coli genome: antagonistic interplay with the universal silencer H-NS.
  5. H-NS and LeuO are antagonistic regulators of CRISPR-based immunity.
    Title: H-NS-mediated repression of CRISPR-based immunity in Escherichia coli K12 can be relieved by the transcription activator LeuO.
  6. The results described herein indicate that the yjcRQP operon, one of the LeuO targets, is involved in sensitivity control against sulfa drugs.
    Title: Involvement of the leucine response transcription factor LeuO in regulation of the genes for sulfa drug efflux.
  7. LeuO and H-NS regulate the expression of the yjjQ-bglJ operon and yjjP.
    Title: Regulation of the yjjQ-bglJ operon, encoding LuxR-type transcription factors, and the divergent yjjP gene by H-NS and LeuO.
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Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables DNA-binding transcription factor activity IEA
Inferred from Electronic Annotation
more info
  
enables sequence-specific DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables sequence-specific DNA binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in regulation of DNA-templated transcription IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within regulation of DNA-templated transcription IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of protein-DNA complex IEA
Inferred from Electronic Annotation
more info
  
part_of protein-DNA complex IPI
Inferred from Physical Interaction
more info
 PubMed 
Preferred Names
DNA-binding transcriptional dual regulator LeuO

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    84368..85312
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414618.4 DNA-binding transcriptional dual regulator LeuO

    See identical proteins and their annotated locations for NP_414618.4

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A376DAV7
    Conserved Domains (1) summary
    PRK09508
    Location:1 → 314
    leuO; leucine transcriptional activator; Reviewed
Nucleotide Protein
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