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carA carbamoyl-phosphate synthetase small subunit [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 949025, updated on 23-Apr-2026
Official Symbol
carA
Official Full Name
carbamoyl-phosphate synthetase small subunit
Primary source
ECOCYC:EG10134
Locus tag
b0032
See related
ASAP:ABE-0000118
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
arg; ECK0033; pyrA
Summary
The small subunit is the amidotransferase component of the enzyme. [More information is available at EcoCyc: EG10134].
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Sequence:
NC_000913.3 (29651..30799)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene ribonucleoside hydrolase RihC Neighboring gene 4-hydroxy-tetrahydrodipicolinate reductase Neighboring gene carbamoyl-phosphate synthetase large subunit Neighboring gene DNA-binding transcriptional activator CaiF

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Repressible expression of an Escherichia coli carA gene inserted into a colicinogenic factor [proceedings]. Crabeel M, et al. Arch Int Physiol Biochim, 1978 Oct. PMID 84616
  2. Uninterrupted translation through putative 12-nucleotide coding gap in sequence of carA: business as usual. Tuohy TM, et al. J Bacteriol, 1994 Jan. PMID 8282707, Free PMC Article
  3. Synthesis of Escherichia coli carbamoylphosphate synthetase initiates at a UUG codon. Weyens G, et al. Eur J Biochem, 1985 Jul 1. PMID 3894020
  4. Regulation of carAB expression in Escherichia coli occurs in part through UTP-sensitive reiterative transcription. Han X, et al. J Bacteriol, 1998 Feb. PMID 9457878, Free PMC Article
  5. The small subunit of carbamoyl phosphate synthetase: snapshots along the reaction pathway. Thoden JB, et al. Biochemistry, 1999 Dec 7. PMID 10587438

See all (70) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. These results indicate that transcription start site sequence and spacing between the -10 region and the start site are important determinants in establishing the extent of reiterative transcription and levels of productive transcription.
    Title: Transcription start site sequence and spacing between the -10 region and the start site affect reiterative transcription-mediated regulation of gene expression in Escherichia coli.
  2. N-terminus verified by Edman degradation on complete protein
    Title: '98 Escherichia coli SWISS-2DPAGE database update.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
contributes_to carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity IBA
Inferred from Biological aspect of Ancestor
more info
  
contributes_to carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity IEA
Inferred from Electronic Annotation
more info
  
contributes_to carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables glutaminase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein heterodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in 'de novo' UMP biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in 'de novo' pyrimidine nucleobase biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within 'de novo' pyrimidine nucleobase biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in L-arginine biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in L-arginine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within L-arginine biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in L-arginine biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in L-arginine biosynthetic process NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in L-glutamine metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within L-glutamine metabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in pyrimidine nucleobase biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of carbamoyl-phosphate synthase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of carbamoyl-phosphate synthase complex IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol HDA PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
carbamoyl-phosphate synthetase small subunit
NP_414573.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    29651..30799
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414573.1 carbamoyl-phosphate synthetase small subunit

    See identical proteins and their annotated locations for NP_414573.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A376FT43
    Conserved Domains (1) summary
    PRK12564
    Location:1 → 374
    PRK12564; carbamoyl-phosphate synthase small subunit
Nucleotide Protein
Heading Accession and Version

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