sufA [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: sufA
Primary source: ECOCYC:EG11378
Locus tag: b1684
See related: ASAP:ABE-0005624
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK1680; ydiC
Summary: iscA, sufA, erpA and nfuA are paralogs. [More information is available at EcoGene: EG11378]. SufA is part of the protein machinery that is involved in the biosynthesis of iron-sulfur clusters. [More information is available at EcoCyc: EG11378].
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Genomic context

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Sequence:: NC_000913.3 (1764018..1764386, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Ferric uptake regulator (Fur) reversibly binds a [2Fe-2S] cluster to sense intracellular iron homeostasis in Escherichia coli.
Title: Ferric uptake regulator (Fur) reversibly binds a [2Fe-2S] cluster to sense intracellular iron homeostasis in Escherichia coli.
deletion of IscA/SufA may disrupt the iron delivery for iron-sulfur cluster biogenesis, therefore impeding sulfur delivery by IscS, and result in the accumulation of red IscS in E. coli cells
Title: Deletion of the Proposed Iron Chaperones IscA/SufA Results in Accumulation of a Red Intermediate Cysteine Desulfurase IscS in Escherichia coli.
Results suggest that the biogenesis of the [4Fe-4S] and the [2Fe-2S] clusters may have distinct pathways and that IscA/SufA paralogues are essential for the [4Fe-4S] cluster assembly, but are dispensable for the [2Fe-2S] cluster assembly.
Title: IscA/SufA paralogues are required for the [4Fe-4S] cluster assembly in enzymes of multiple physiological pathways in Escherichia coli under aerobic growth conditions.
Under aerobiosis, IscA and SufA are functionally redundant carriers and both can receive an Fe/S cluster from IscU.
Title: Iron-sulfur (Fe/S) protein biogenesis: phylogenomic and genetic studies of A-type carriers.
SufA may constitute the redundant cellular activities to recruit intracellular iron and deliver iron for the iron-sulfur cluster assembly in E. coli.
Title: Complementary roles of SufA and IscA in the biogenesis of iron-sulfur clusters in Escherichia coli.
SufA exists as a homodimer; four cysteine residues in the Cys-Gly-Cys motif are positioned in close proximity at the dimer interface; these cysteines together with the nearby Glu118 may allow coordination of an Fe-S cluster and/or an Fe atom.
Title: Crystal structure of Escherichia coli SufA involved in biosynthesis of iron-sulfur clusters: implications for a functional dimer.
SufA transcription was activated directly by binding of IscR and OxyR to the promoter.
Title: IscR acts as an activator in response to oxidative stress for the suf operon encoding Fe-S assembly proteins.

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General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables 2 iron, 2 sulfur cluster binding	IBA Inferred from Biological aspect of Ancestor more info
enables 2 iron, 2 sulfur cluster binding	IDA Inferred from Direct Assay more info	PubMed
enables 2 iron, 2 sulfur cluster binding	IEA Inferred from Electronic Annotation more info
enables 4 iron, 4 sulfur cluster binding	IEA Inferred from Electronic Annotation more info
enables iron-sulfur cluster binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in iron-sulfur cluster assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in iron-sulfur cluster assembly	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within iron-sulfur cluster assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein maturation by iron-sulfur cluster transfer	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein maturation by iron-sulfur cluster transfer	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within response to oxidative stress	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to oxidative stress	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
part_of iron-sulfur cluster transfer complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: iron-sulfur cluster insertion protein SufA

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

1764018..1764386 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_416199.1 iron-sulfur cluster insertion protein SufA [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_416199.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A5B9AI91

Conserved Domains (1) summary

PRK09504
Location:1 → 122

sufA; iron-sulfur cluster assembly scaffold protein; Provisional