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sufA iron-sulfur cluster insertion protein SufA [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 949014, updated on 26-Mar-2025
Official Symbol
sufA
Official Full Name
iron-sulfur cluster insertion protein SufA
Primary source
ECOCYC:EG11378
Locus tag
b1684
See related
ASAP:ABE-0005624
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1680; ydiC
Summary
iscA, sufA, erpA and nfuA are paralogs. [More information is available at EcoGene: EG11378]. SufA is part of the protein machinery that is involved in the biosynthesis of iron-sulfur clusters. [More information is available at EcoCyc: EG11378].
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Sequence:
NC_000913.3 (1764018..1764386, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene Fe-S cluster scaffold complex subunit SufD Neighboring gene Fe-S cluster scaffold complex subunit SufC Neighboring gene Fe-S cluster scaffold complex subunit SufB Neighboring gene ncRNA Neighboring gene uncharacterized protein YdiH

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Native Escherichia coli SufA, coexpressed with SufBCDSE, purifies as a [2Fe-2S] protein and acts as an Fe-S transporter to Fe-S target enzymes. Gupta V, et al. J Am Chem Soc, 2009 May 6. PMID 19366265, Free PMC Article
  2. Assignment of the (1)H, (15)N and (13)C resonances of SufA from Escherichia coli involved in Fe-S cluster biosynthesis. Duraffourg N, et al. J Biomol NMR, 2004 Nov. PMID 15754061
  3. Deletion of the Proposed Iron Chaperones IscA/SufA Results in Accumulation of a Red Intermediate Cysteine Desulfurase IscS in Escherichia coli. Yang J, et al. J Biol Chem, 2015 May 29. PMID 25907559, Free PMC Article
  4. A suf operon requirement for Fe-S cluster assembly during iron starvation in Escherichia coli. Outten FW, et al. Mol Microbiol, 2004 May. PMID 15101990
  5. Ferric uptake regulator (Fur) reversibly binds a [2Fe-2S] cluster to sense intracellular iron homeostasis in Escherichia coli. Fontenot CR, et al. J Biol Chem, 2020 Nov 13. PMID 32928958, Free PMC Article

See all (33) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Ferric uptake regulator (Fur) reversibly binds a [2Fe-2S] cluster to sense intracellular iron homeostasis in Escherichia coli.
    Title: Ferric uptake regulator (Fur) reversibly binds a [2Fe-2S] cluster to sense intracellular iron homeostasis in Escherichia coli.
  2. deletion of IscA/SufA may disrupt the iron delivery for iron-sulfur cluster biogenesis, therefore impeding sulfur delivery by IscS, and result in the accumulation of red IscS in E. coli cells
    Title: Deletion of the Proposed Iron Chaperones IscA/SufA Results in Accumulation of a Red Intermediate Cysteine Desulfurase IscS in Escherichia coli.
  3. Results suggest that the biogenesis of the [4Fe-4S] and the [2Fe-2S] clusters may have distinct pathways and that IscA/SufA paralogues are essential for the [4Fe-4S] cluster assembly, but are dispensable for the [2Fe-2S] cluster assembly.
    Title: IscA/SufA paralogues are required for the [4Fe-4S] cluster assembly in enzymes of multiple physiological pathways in Escherichia coli under aerobic growth conditions.
  4. Under aerobiosis, IscA and SufA are functionally redundant carriers and both can receive an Fe/S cluster from IscU.
    Title: Iron-sulfur (Fe/S) protein biogenesis: phylogenomic and genetic studies of A-type carriers.
  5. SufA may constitute the redundant cellular activities to recruit intracellular iron and deliver iron for the iron-sulfur cluster assembly in E. coli.
    Title: Complementary roles of SufA and IscA in the biogenesis of iron-sulfur clusters in Escherichia coli.
  6. SufA exists as a homodimer; four cysteine residues in the Cys-Gly-Cys motif are positioned in close proximity at the dimer interface; these cysteines together with the nearby Glu118 may allow coordination of an Fe-S cluster and/or an Fe atom.
    Title: Crystal structure of Escherichia coli SufA involved in biosynthesis of iron-sulfur clusters: implications for a functional dimer.
  7. SufA transcription was activated directly by binding of IscR and OxyR to the promoter.
    Title: IscR acts as an activator in response to oxidative stress for the suf operon encoding Fe-S assembly proteins.
Submit: New GeneRIF Correction

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 2 iron, 2 sulfur cluster binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 2 iron, 2 sulfur cluster binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables 2 iron, 2 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables 4 iron, 4 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables iron-sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in iron-sulfur cluster assembly IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in iron-sulfur cluster assembly IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within iron-sulfur cluster assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein maturation IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within response to oxidative stress IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within response to oxidative stress IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of iron-sulfur cluster transfer complex IPI
Inferred from Physical Interaction
more info
 PubMed 
Preferred Names
iron-sulfur cluster insertion protein SufA

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1764018..1764386 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416199.1 iron-sulfur cluster insertion protein SufA [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416199.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9AI91
    Conserved Domains (1) summary
    PRK09504
    Location:1 → 122
    sufA; iron-sulfur cluster assembly scaffold protein; Provisional
Nucleotide Protein
Heading Accession and Version

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