rob [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: rob
Primary source: ECOCYC:EG11366
Locus tag: b4396
See related: ASAP:ABE-0014420
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: cbpB; ECK4388; robA
Summary: Rob binds to right border of the oriC origin of chromsome replication. [More information is available at EcoGene: EG11366]. Rob is a protein that belongs to the AraC/XylS family. [More information is available at EcoCyc: EG11366].
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Genomic context

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Sequence:: NC_000913.3 (4634441..4635310, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Structural basis of transcription activation by Rob, a pleiotropic AraC/XylS family regulator.
Title: Structural basis of transcription activation by Rob, a pleiotropic AraC/XylS family regulator.
The N-terminal Helix-Turn-Helix Motif of Transcription Factors MarA and Rob Drives DNA Recognition.
Title: The N-terminal Helix-Turn-Helix Motif of Transcription Factors MarA and Rob Drives DNA Recognition.
Effects of Nucleobase Amino Acids on the Binding of Rob to Its Promoter DNA: Differential Alteration of DNA Affinity and Phenotype.
Title: Effects of Nucleobase Amino Acids on the Binding of Rob to Its Promoter DNA: Differential Alteration of DNA Affinity and Phenotype.
Study employs the X-ray crystal structure of transcriptional regulator Rob bound to its micF promoter. Rob proteins modified in vivo with nucleobase amino acids at position 40 exhibited altered DNA promoter binding with promoter DNA A-box residue Gua-6. Rob protein expression ultimately controls phenotypic changes, including resistance to antibiotics.
Title: Alteration of Transcriptional Regulator Rob In Vivo: Enhancement of Promoter DNA Binding and Antibiotic Resistance in the Presence of Nucleobase Amino Acids.
We speculate that replication pausing and fork-slow-down shortly after initiation may represent a novel checkpoint that ensures the presence of sufficient nucleotide supply prior to commitment to duplication of the entire genome.
Title: Fork restart protein, PriA, binds around oriC after depletion of nucleotide precursors: Replication fork arrest near the replication origin.
study found that although Rob makes fewer interactions with sigma(70) R4 than SoxS, the 2 proteins make the same position-dependent interactions; Rob occludes sigma(70) R4 from binding the -35 hexamer, just as does SoxS; thus, the C-terminal domain does not substantially alter the way Rob interacts with sigma(70) R4 at class II promoters
Title: Transcription activation by Escherichia coli Rob at class II promoters: protein-protein interactions between Rob's N-terminal domain and the σ(70) subunit of RNA polymerase.
roles of the Escherichia coli transcriptional regulators MarA, SoxS and Rob in susceptibility to cationic antimicrobial peptides
Title: Different effects of transcriptional regulators MarA, SoxS and Rob on susceptibility of Escherichia coli to cationic antimicrobial peptides (CAMPs): Rob-dependent CAMP induction of the marRAB operon.
Data that TolC is involved in the efflux of certain cellular metabolites that accumulate during growth and trigger the upregulation of MarA, SoxS, and Rob, which in turn upregulate tolC and help rid the bacteria of these metabolites.
Title: An excretory function for the Escherichia coli outer membrane pore TolC: upregulation of marA and soxS transcription and Rob activity due to metabolites accumulated in tolC mutants.
The authors propose that sequestration inactivates Rob by blocking its access to the transcriptional machinery and that inducers activate Rob by mediating its dispersal, allowing interaction with RNA polymerase.
Title: Two functions of the C-terminal domain of Escherichia coli Rob: mediating "sequestration-dispersal" as a novel off-on switch for regulating Rob's activity as a transcription activator and preventing degradation of Rob by Lon protease.
The authors show here that tolC has two additional strong overlapping promoters, p3 and p4, which are downstream of p1, p2 and the marbox and are activated by MarA, SoxS and Rob.
Title: Transcriptional activation by MarA, SoxS and Rob of two tolC promoters using one binding site: a complex promoter configuration for tolC in Escherichia coli.

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General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity	IEA Inferred from Electronic Annotation more info
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: DNA-binding transcriptional dual regulator Rob

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

4634441..4635310 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_418813.1 DNA-binding transcriptional dual regulator Rob [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_418813.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A7A6TXV0, A0A8S7EJM1, A0A8T5ZCK8

Conserved Domains (1) summary

PRK15121
Location:1 → 289

PRK15121; MDR efflux pump AcrAB transcriptional activator RobA