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hsdM type I restriction enzyme EcoKI methylase subunit [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948872, updated on 6-Jul-2026
Official Symbol
hsdM
Official Full Name
type I restriction enzyme EcoKI methylase subunit
Primary source
ECOCYC:EG10458
Locus tag
b4349
See related
ASAP:ABE-0014260
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4339; hsm; hsp
Summary
HsdM is the modification methyltransferase component of the EcoKI restriction-modification system . [More information is available at EcoCyc: EG10458].
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Sequence:
NC_000913.3 (4581459..4583048, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene Type IV methyl-directed restriction enzyme EcoKMcrBC subunit Neighboring gene 5-methylcytosine-specific restriction enzyme subunit McrB;mcrB-S Neighboring gene nucleoid-associated protein SymE Neighboring gene ncRNA Neighboring gene type I restriction enzyme EcoKI specificity subunit Neighboring gene type I restriction enzyme EcoKI endonuclease subunit Neighboring gene Type IV methyl-directed restriction enzyme EcoKMrr

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Purification and characterization of the methyltransferase from the type 1 restriction and modification system of Escherichia coli K12. Dryden DT, et al. J Biol Chem, 1993 Jun 25. PMID 8514761
  2. Method for isolating restriction- and modificationless mutants of Escherichia coli K-12. Del Giudice L. J Bacteriol, 1979 Jan. PMID 368038, Free PMC Article
  3. Organization and sequence of the hsd genes of Escherichia coli K-12. Loenen WA, et al. J Mol Biol, 1987 Nov 20. PMID 3323532
  4. Restriction and modification of DNA by a complex protein. Yuan R, et al. Am Sci, 1982 Jan-Feb. PMID 6282152
  5. Substrate recognition and selectivity in the type IC DNA modification methylase M.EcoR124I. Taylor I, et al. Nucleic Acids Res, 1993 Oct 25. PMID 8177741, Free PMC Article

See all (25) citations in PubMed

GeneRIFs: Gene References Into Functions

Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables N-methyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables N-methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables N-methyltransferase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables nucleic acid binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables site-specific DNA-methyltransferase (adenine-specific) activity EXP
Inferred from Experiment
more info
 PubMed 
enables site-specific DNA-methyltransferase (adenine-specific) activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in DNA restriction-modification system IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in DNA restriction-modification system IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of type I site-specific deoxyribonuclease complex IPI
Inferred from Physical Interaction
more info
 PubMed 
Preferred Names
type I restriction enzyme EcoKI methylase subunit
NP_418769.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4581459..4583048 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418769.1 type I restriction enzyme EcoKI methylase subunit

    See identical proteins and their annotated locations for NP_418769.1

    Status: REVIEWED

    Conserved Domains (2) summary
    pfam02384
    Location:122 → 450
    N6_Mtase; N-6 DNA Methylase
    pfam12161
    Location:6 → 101
    HsdM_N; HsdM N-terminal domain
Nucleotide Protein
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