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dnaC DNA biosynthesis protein [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948864, updated on 22-May-2018

Summary

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Gene symbol
dnaC
Gene description
DNA biosynthesis protein
Primary source
EcoGene:EG10237
Locus tag
b4361
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
dnaD; ECK4351; JW4325
Summary
DnaC is an accessory protein that loads the DnaB replicative helicase onto duplex DNA to initiate replication and onto single-stranded DNA to assist in primer formation by primase . [More information is available at EcoCyc: EG10237].

Genomic context

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Sequence:
NC_000913.3 (4600238..4600975, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene OPG periplasmic biosynthetic phosphoglycerol transferases I (membrane-bound) and II (soluble) Neighboring gene putative DUF2501 family periplasmic protein Neighboring gene DNA biosynthesis protein (primosomal protein I) Neighboring gene DUF3815 family inner membrane protein Neighboring gene DUF1212 family inner membrane protein

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Genetic method to analyze essential genes of Escherichia coli. Hupert-Kocurek K, et al. Appl Environ Microbiol, 2007 Nov. PMID 17873068, Free PMC Article
  2. The nucleotide-binding site of the Escherichia coli DnaC protein: molecular topography of DnaC protein-nucleotide cofactor complexes. Galletto R, et al. Cell Biochem Biophys, 2005. PMID 16244362
  3. Kinetics of allosteric conformational transition of a macromolecule prior to ligand binding: analysis of stopped-flow kinetic experiments. Galletto R, et al. Cell Biochem Biophys, 2005. PMID 15858229
  4. Global conformation of the Escherichia coli replication factor DnaC protein in absence and presence of nucleotide cofactors. Galletto R, et al. Biochemistry, 2004 Aug 31. PMID 15323558
  5. A dnaC mutation in Escherichia coli that affects copy number of ColE1-like plasmids and the PriA-PriB (but not Rep-PriC) pathway of chromosomal replication restart. Harinarayanan R, et al. Genetics, 2004 Mar. PMID 15082538, Free PMC Article

See all (84) citations in PubMed

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

  1. Thus, imbalances in levels of the replicative helicase and helicase loader can inhibit replication both via inhibition of DnaB6-DnaC6 complex formation with excess DnaB, as shown here, and promotion of formation of DnaB6-DnaC6 complexes with excess DnaC.
    Title: Overexpression of the Replicative Helicase in Escherichia coli Inhibits Replication Initiation and Replication Fork Reloading.
  2. Data demonstrate that DnaC opens DnaB by a mechanism akin to that of polymerase clamp loaders and indicate that bacterial replicative helicases, like their eukaryotic counterparts, possess autoregulatory elements that influence how hexameric motor domains are loaded onto and unwind DNA.
    Title: The bacterial DnaC helicase loader is a DnaB ring breaker.
  3. N-terminus verified by Edman degradation on complete protein
    Title: Structure of Escherichia coli dnaC. Identification of a cysteine residue possibly involved in association with dnaB protein.
  4. The complementation of EcDnaC function by HpDnaB in vivo, suggesting that HpDnaB can bypass EcDnaC activity in vivo.
    Title: Helicobacter pylori DnaB helicase can bypass Escherichia coli DnaC function in vivo.
  5. A new dnaC mutation (dnaC824) is reported here that efficiently suppresses priB rep mutant phenotypes.
    Title: A novel dnaC mutation that suppresses priB rep mutant phenotypes in Escherichia coli K-12.
  6. These results suggest that in the presence of ATP in vivo, the DnaB6 x DnaC6 complex should be more efficient in binding DNA as well as in loading DnaB onto the ssDNA than DnaB helicase itself.
    Title: Quantitative analysis of binding of single-stranded DNA by Escherichia coli DnaB helicase and the DnaB x DnaC complex.
Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by GO

Function Evidence Code Pubs
protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
DNA replication IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
DNA strand elongation involved in DNA replication IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
DNA strand elongation involved in DNA replication IGI
Inferred from Genetic Interaction
more info
 PubMed 

General protein information

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Names
DNA biosynthesis protein
NP_418781.1
  • chromosome replication; initiation and chain elongation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4600238..4600975 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418781.1 DNA biosynthesis protein [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418781.1

    Status: PROVISIONAL

    UniProtKB/Swiss-Prot
    P0AEF0
    Conserved Domains (1) summary
    PRK07952
    Location:1 → 244
    PRK07952; DNA replication protein DnaC; Validated
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

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Other Literature Sources
Molecular Biology Databases
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