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iraD anti-adaptor protein IraD [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948851, updated on 19-Sep-2024

Summary

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Official Symbol
iraD
Official Full Name
anti-adaptor protein IraD
Primary source
ECOCYC:G7923
Locus tag
b4326
See related
ASAP:ABE-0014190
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4317; yjiD
Summary
IraD inhibits proteolysis of the stationary phase sigma factor S by interacting with RssB, which would normally target S for degradation by the ClpXP protease . [More information is available at EcoCyc: G7923].
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Genomic context

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Sequence:
NC_000913.3 (4556993..4557385)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF2686 domain-containing protein YjiC Neighboring gene protein YtiC Neighboring gene protein YtiD Neighboring gene iraD leader peptide Neighboring gene DNA-binding transcriptional dual regulator HypT Neighboring gene isoaspartyl dipeptidase Neighboring gene Gate family protein YjiG Neighboring gene uncharacterized protein YjiH

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Structural basis for inhibition of a response regulator of σ(S) stability by a ClpXP antiadaptor. Dorich V, et al. Genes Dev, 2019 Jun 1. PMID 30975721, Free PMC Article
  2. A DNA damage response in Escherichia coli involving the alternative sigma factor, RpoS. Merrikh H, et al. Proc Natl Acad Sci U S A, 2009 Jan 13. PMID 19124769, Free PMC Article
  3. Translational Repression of the RpoS Antiadapter IraD by CsrA Is Mediated via Translational Coupling to a Short Upstream Open Reading Frame. Park H, et al. mBio, 2017 Aug 29. PMID 28851853, Free PMC Article
  4. Anti-adaptors use distinct modes of binding to inhibit the RssB-dependent turnover of RpoS (σ(S)) by ClpXP. Micevski D, et al. Front Mol Biosci, 2015. PMID 25988182, Free PMC Article
  5. Growth phase and (p)ppGpp control of IraD, a regulator of RpoS stability, in Escherichia coli. Merrikh H, et al. J Bacteriol, 2009 Dec. PMID 19820090, Free PMC Article

See all (14) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. crystal structure of RssB bound to an antiadaptor, the DNA damage-inducible IraD; demonstrate that (1) in the presence of IraD, RssB adopts predominantly a closed conformation; (2) IraD contacts both domains of RssB; (3) the interactions observed in the structure are crucial for blocking sigmaS degradation in vivo and in vitro
    Title: Structural basis for inhibition of a response regulator of σ(S) stability by a ClpXP antiadaptor.
  2. findings show that CsrA employs a novel regulatory mechanism to repress translation of iraD; CsrA binds to four sites in the iraD leader transcript but does not directly occlude ribosome binding to the iraD SD sequence; instead, CsrA represses translation of a short open reading frame encoded upstream of iraD, causing repression of iraD translation
    Title: Translational Repression of the RpoS Antiadapter IraD by CsrA Is Mediated via Translational Coupling to a Short Upstream Open Reading Frame.
  3. iraD transcription is induced by DNA damage by a mechanism independent of the SOS response; IraD promoteS accumulation of the alternative transcription sigma factor, RpoS
    Title: A DNA damage response in Escherichia coli involving the alternative sigma factor, RpoS.
  4. These results suggest that the induction of RpoS during transition to stationary phase and by (p)ppGpp occurs at least partially through IraD.
    Title: Growth phase and (p)ppGpp control of IraD, a regulator of RpoS stability, in Escherichia coli.
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General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables anti-sigma factor antagonist activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables anti-sigma factor antagonist activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA damage response IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within DNA damage response IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in cellular response to oxidative stress IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within negative regulation of protein catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
anti-adaptor protein IraD

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4556993..4557385
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418746.4 anti-adaptor protein IraD [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418746.4

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8E0KUI3
    Conserved Domains (1) summary
    PRK14128
    Location:54 → 122
    iraD; anti-adapter protein IraD
Nucleotide Protein
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