U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

fimD type I fimbriae usher protein [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948844, updated on 30-Jul-2025
Official Symbol
fimD
Official Full Name
type I fimbriae usher protein
Primary source
ECOCYC:EG10311
Locus tag
b4317
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4308; fimD_1; fimD_2
Summary
FimD forms pores in the outer membrane. [More information is available at EcoGene: EG10311]. FimD is a member of the Fimbrial Usher Porin (FUP) family . [More information is available at EcoCyc: EG10311].
Try the new Gene page
Try the new Transcripts and proteins table
Sequence:
NC_000913.3 (4545096..4547732)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative fimbrial protein FimI Neighboring gene type 1 fimbriae periplasmic chaperone Neighboring gene type 1 fimbriae minor subunit FimF Neighboring gene type 1 fimbriae minor subunit FimG

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Allosteric signalling in the outer membrane translocation domain of PapC usher. Farabella I, et al. Elife, 2014 Oct 28. PMID 25271373, Free PMC Article
  2. The usher N terminus is the initial targeting site for chaperone-subunit complexes and participates in subsequent pilus biogenesis events. Ng TW, et al. J Bacteriol, 2004 Aug. PMID 15292133, Free PMC Article
  3. Conformational Dynamics, Intramolecular Domain Conformation Signaling, and Activation of Apo-FimD Revealed by Single-Molecule Fluorescence Resonance Energy Transfer Studies. Liu Y, et al. Biochemistry, 2019 Apr 9. PMID 30888187
  4. Reconstitution of pilus assembly reveals a bacterial outer membrane catalyst. Nishiyama M, et al. Science, 2008 Apr 18. PMID 18369105
  5. The PapC usher forms an oligomeric channel: implications for pilus biogenesis across the outer membrane. Thanassi DG, et al. Proc Natl Acad Sci U S A, 1998 Mar 17. PMID 9501230, Free PMC Article

See all (48) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Data show that the substrate-free usher FimD (apo-FimD) adopts a contracted conformation that is distinct from its substrate-bound states.
    Title: Conformational Dynamics, Intramolecular Domain Conformation Signaling, and Activation of Apo-FimD Revealed by Single-Molecule Fluorescence Resonance Energy Transfer Studies.
  2. This study describes the gating mechanism of PapC ushers and its importance in maintaining outer-membrane permeability.
    Title: Allosteric signalling in the outer membrane translocation domain of PapC usher.
  3. The results indicate that FimD usher follows the SurA-BamB pathway for its assembly.
    Title: The fimbrial usher FimD follows the SurA-BamB pathway for its assembly in the outer membrane of Escherichia coli.
  4. The catalytic activity of FimD is required for extension of the pilus fiber.
    Title: Function of the usher N-terminus in catalysing pilus assembly.
  5. These data indicate that residues G15, N46 and D47 play an important role following initial binding of FimH to FimD for efficient type 1 fimbriae polymerization by this outer membrane usher.
    Title: Specific residues in the N-terminal domain of FimH stimulate type 1 fimbriae assembly in Escherichia coli following the initial binding of the adhesin to FimD usher.
  6. PapC and FimD of Uropathogenic E coli are ushers bound to a translocating type 1 pilus assembly intermediate; they capture the secretion of a virulence factor across the outer membrane.
    Title: Fiber formation across the bacterial outer membrane by the chaperone/usher pathway.
  7. study of reconstituted type 1 pilus biogenesis from purified pilus proteins; the usher FimD acted as a catalyst to accelerate the ordered assembly of protein subunits independently of cellular energy
    Title: Reconstitution of pilus assembly reveals a bacterial outer membrane catalyst.
  8. N-terminus verified by Edman degradation on mature peptide
    Title: Identification and characterization of the chaperone-subunit complex-binding domain from the type 1 pilus assembly platform FimD.
  9. The lectin domain of FimH recognized by FimD usher is an essential step for type 1 pilus biogenesis.
    Title: Recognition of the N-terminal lectin domain of FimH adhesin by the usher FimD is required for type 1 pilus biogenesis.
Submit: New GeneRIF Correction

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables fimbrial usher porin activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables fimbrial usher porin activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables fimbrial usher porin activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in pilus assembly IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within pilus assembly IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in pilus assembly IEA
Inferred from Electronic Annotation
more info
  
involved_in transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in cell outer membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cell outer membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cell outer membrane IEA
Inferred from Electronic Annotation
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
type I fimbriae usher protein

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4545096..4547732
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418737.1 type I fimbriae usher protein

    See identical proteins and their annotated locations for NP_418737.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A8S7EI66
    Conserved Domains (1) summary
    COG3188
    Location:11 → 878
    FimD; Outer membrane usher protein FimD/PapC [Cell motility, Extracellular structures]
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.