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holC DNA polymerase III subunit chi [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948787, updated on 26-Mar-2025
Official Symbol
holC
Official Full Name
DNA polymerase III subunit chi
Primary source
ECOCYC:EG11413
Locus tag
b4259
See related
ASAP:ABE-0013948
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4252
Summary
The preinitiation complex binds primed ssDNA and loads the beta processivity clamp. [More information is available at EcoCyc: EG11413].
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Sequence:
NC_000913.3 (4483837..4484280, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene inner membrane protein YjgN Neighboring gene valine--tRNA ligase Neighboring gene aminopeptidase A/I Neighboring gene protein YtgB

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. The Role of Replication Clamp-Loader Protein HolC of Escherichia coli in Overcoming Replication/Transcription Conflicts. Cooper DL, et al. mBio, 2021 Mar 9. PMID 33688004, Free PMC Article
  2. Site-directed mutagenesis of the χ subunit of DNA polymerase III and single-stranded DNA-binding protein of E. coli reveals key residues for their interaction. Naue N, et al. Nucleic Acids Res, 2011 Mar. PMID 20972214, Free PMC Article
  3. Characterization of the Escherichia coli XPD/Rad3 iron-sulfur helicase YoaA in complex with the DNA polymerase III clamp loader subunit chi (χ). Weeks-Pollenz SJ, et al. J Biol Chem, 2023 Jan. PMID 36509145, Free PMC Article
  4. Identification, molecular cloning and characterization of the gene encoding the chi subunit of DNA polymerase III holoenzyme of Escherichia coli. Carter JR, et al. Mol Gen Genet, 1993 Nov. PMID 8246893
  5. Genetic Analysis of DinG Family Helicase YoaA and Its Interaction with Replication Clamp Loader Protein HolC in Escherichia coli. Sutera VA, et al. J Bacteriol, 2021 Aug 20. PMID 34181484, Free PMC Article

See all (92) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Characterization of the Escherichia coli XPD/Rad3 iron-sulfur helicase YoaA in complex with the DNA polymerase III clamp loader subunit chi (chi).
    Title: Characterization of the Escherichia coli XPD/Rad3 iron-sulfur helicase YoaA in complex with the DNA polymerase III clamp loader subunit chi (χ).
  2. Genetic Analysis of DinG Family Helicase YoaA and Its Interaction with Replication Clamp Loader Protein HolC in Escherichia coli.
    Title: Genetic Analysis of DinG Family Helicase YoaA and Its Interaction with Replication Clamp Loader Protein HolC in Escherichia coli.
  3. The Role of Replication Clamp-Loader Protein HolC of Escherichia coli in Overcoming Replication/Transcription Conflicts.
    Title: The Role of Replication Clamp-Loader Protein HolC of Escherichia coli in Overcoming Replication/Transcription Conflicts.
  4. This increased MMS sensitivity was not suppressed by the presence of multi-copy holC. These results indicate that yoaA is involved in MMS sensitivity and suggest that YoaA functions together with HolC.
    Title: Systematic identification of synthetic lethal mutations with reduced-genome Escherichia coli: synthetic genetic interactions among yoaA, xthA and holC related to survival from MMS exposure.
  5. Results support the idea that controlling the balance between SSB binding modes is critical for DNA Pol III holoenzyme stability.
    Title: ssb gene duplication restores the viability of ΔholC and ΔholD Escherichia coli mutants.
  6. evolutionary conserved residues of chi form a hydrophobic pocket to accommodate the ultimate two amino acids of single-stranded DNA-binding protein, P176 and F177.
    Title: Site-directed mutagenesis of the χ subunit of DNA polymerase III and single-stranded DNA-binding protein of E. coli reveals key residues for their interaction.
  7. N-terminus verified by Edman degradation on complete protein
    Title: DNA polymerase III accessory proteins. III. holC and holD encoding chi and psi.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA polymerase activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA-directed DNA polymerase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA-directed DNA polymerase activity IEA
Inferred from Electronic Annotation
more info
  
enables nucleotidyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in DNA biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA replication IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA replication NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in DNA-templated DNA replication NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in positive regulation of DNA-templated DNA replication initiation IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within positive regulation of DNA-templated DNA replication initiation IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within response to radiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of DNA polymerase III complex NAS
Non-traceable Author Statement
more info
 PubMed 
part_of DNA polymerase III, clamp loader complex IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of replisome NAS
Non-traceable Author Statement
more info
 PubMed 
Preferred Names
DNA polymerase III subunit chi

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4483837..4484280 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418680.1 DNA polymerase III subunit chi [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418680.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A099AUD6, A0A0H3F3I3, A0A8E0FI71, A0AAN3V2C8
    Conserved Domains (1) summary
    PRK05728
    Location:1 → 147
    PRK05728; DNA polymerase III subunit chi; Validated
Nucleotide Protein
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