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priB [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948722, updated on 11-Sep-2024

Summary

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Official Symbol
priB
Primary source
ECOCYC:EG10764
Locus tag
b4201
See related
ASAP:ABE-0013742
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4197
Summary
PriB is structural similar to Ssb. [More information is available at EcoGene: EG10764]. PriB is a component of the primosome, a multiprotein complex that is believed to be involved in restart of stalled replication forks. [More information is available at EcoCyc: EG10764].
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Genomic context

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Sequence:
NC_000913.3 (4425520..4425834)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF1471 domain-containing protein YjfY Neighboring gene 30S ribosomal subunit protein S6 Neighboring gene 30S ribosomal subunit protein S18 Neighboring gene 50S ribosomal subunit protein L9

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Involvement of histidine in complex formation of PriB and single-stranded DNA. Fujiyama S, et al. Biochim Biophys Acta, 2014 Feb. PMID 24200676
  2. The priB gene of Klebsiella pneumoniae encodes a 104-amino acid protein that is similar in structure and function to Escherichia coli PriB. Berg L, et al. PLoS One, 2011. PMID 21931731, Free PMC Article
  3. Complexed crystal structure of replication restart primosome protein PriB reveals a novel single-stranded DNA-binding mode. Huang CY, et al. Nucleic Acids Res, 2006. PMID 16899446, Free PMC Article
  4. Crystal structure of a biologically functional form of PriB from Escherichia coli reveals a potential single-stranded DNA-binding site. Shioi S, et al. Biochem Biophys Res Commun, 2005 Jan 28. PMID 15607735
  5. Crystal structure of PriB, a primosomal DNA replication protein of Escherichia coli. Liu JH, et al. J Biol Chem, 2004 Nov 26. PMID 15383524

See all (41) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Identification of genetic interactions with priB links the PriA/PriB DNA replication restart pathway to double-strand DNA break repair in Escherichia coli.
    Title: Identification of genetic interactions with priB links the PriA/PriB DNA replication restart pathway to double-strand DNA break repair in Escherichia coli.
  2. A structural model of the PriB-DnaT complex in Escherichia coli replication restart.
    Title: A structural model of the PriB-DnaT complex in Escherichia coli replication restart.
  3. Structural insight into the DNA-binding mode of the primosomal proteins PriA, PriB, and DnaT.
    Title: Structural insight into the DNA-binding mode of the primosomal proteins PriA, PriB, and DnaT.
  4. found that the acidic linker between the two domains had an activity for dissociating ssDNA from the PriB.ssDNA complexes in a manner supported by the conserved acidic residues Asp70 and Glu76
    Title: Structure and mechanism of the primosome protein DnaT-functional structures for homotrimerization, dissociation of ssDNA from the PriB·ssDNA complex, and formation of the DnaT·ssDNA complex.
  5. A role for His64 in the compactness of the PriB-ssDNA complex and in the positive cooperativity of PriB.
    Title: Involvement of histidine in complex formation of PriB and single-stranded DNA.
  6. Abolishing PriB leads to suppression of the recG null phenotype.
    Title: Modulation of DNA damage tolerance in Escherichia coli recG and ruv strains by mutations affecting PriB, the ribosome and RNA polymerase.
  7. Binding mode is proposed for forming a stable complex of PriB with ssDNA of 25 nucleotides.
    Title: A single residue determines the cooperative binding property of a primosomal DNA replication protein, PriB, to single-stranded DNA.
  8. PriB homolog of K. pneumoniae is similar in structure and in function to that of E. coli
    Title: The priB gene of Klebsiella pneumoniae encodes a 104-amino acid protein that is similar in structure and function to Escherichia coli PriB.
  9. Binding of the PriB dimer to the PriA- primosome assembly site complex dramatically increases PriA's affinity for the strong site, but only slightly affects its affinity for the weak site.
    Title: Binding of two PriA-PriB complexes to the primosome assembly site initiates primosome formation.
  10. N-terminus verified by Edman degradation on mature peptide
    Title: The priB and priC replication proteins of Escherichia coli. Genes, DNA sequence, overexpression, and purification.
Submit: New GeneRIF Correction See all GeneRIFs (15)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables single-stranded DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables single-stranded DNA binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in DNA replication IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within DNA replication initiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in DNA replication, synthesis of primer IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA replication, synthesis of primer NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in DNA unwinding involved in DNA replication NAS
Non-traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within plasmid maintenance IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in replication fork processing NAS
Non-traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within response to radiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of DnaB-DnaC-DnaT-PriA-PriB complex NAS
Non-traceable Author Statement
more info
 PubMed 
part_of pre-primosome complex IMP
Inferred from Mutant Phenotype
more info
 PubMed 
part_of primosome complex IEA
Inferred from Electronic Annotation
more info
  
part_of primosome complex NAS
Non-traceable Author Statement
more info
 PubMed 
part_of replisome IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
primosomal replication protein N

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4425520..4425834
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418622.1 primosomal replication protein N [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418622.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5C9A7U1
    Conserved Domains (1) summary
    PRK02801
    Location:1 → 101
    PRK02801; primosomal replication protein N; Provisional
Nucleotide Protein
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