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miaA tRNA dimethylallyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948690, updated on 26-Mar-2025

Summary

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Official Symbol
miaA
Official Full Name
tRNA dimethylallyltransferase
Primary source
ECOCYC:EG10595
Locus tag
b4171
See related
ASAP:ABE-0013657
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4167; trpX
Summary
(2)-isopentenylpyrophosphate tRNA-adenosine transferase (MiaA) isopentenylates several tRNAs, a modification that is important in preventing frameshifts and other mutations. [More information is available at EcoCyc: EG10595].
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Genomic context

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Sequence:
NC_000913.3 (4399252..4400202)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene NAD(P)HX epimerase/NAD(P)HX dehydratase Neighboring gene N(6)-L-threonylcarbamoyladenine synthase, TsaE subunit Neighboring gene N-acetylmuramoyl-L-alanine amidase B Neighboring gene DNA mismatch repair protein MutL Neighboring gene RNA-binding protein Hfq Neighboring gene ribosome rescue factor HflX

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. tRNA modification activity is necessary for Tet(M)-mediated tetracycline resistance. Burdett V. J Bacteriol, 1993 Nov. PMID 8226667, Free PMC Article
  2. The effect of an Escherichia coli regulatory mutation on transfer RNA structure. Eisenberg SP, et al. J Mol Biol, 1979 Nov 25. PMID 93644
  3. Characterization of a deficiency of N6-(delta 2-isopentenyl)-2-methylthioadenosine in the Escherichia coli mutant trpX by use of antibodies to N6-(delta 2-isopentenyl)adenosine. Vold BS, et al. J Biol Chem, 1979 Aug 10. PMID 379002
  4. Replacement of the Escherichia coli trp operon attenuation control codons alters operon expression. Landick R, et al. J Mol Biol, 1990 Nov 5. PMID 2231731
  5. Genetic and physiological relationships among the miaA gene, 2-methylthio-N6-(delta 2-isopentenyl)-adenosine tRNA modification, and spontaneous mutagenesis in Escherichia coli K-12. Connolly DM, et al. J Bacteriol, 1989 Jun. PMID 2656644, Free PMC Article

See all (49) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Dependence on MiaA may therefore provide yet another way for RpoS levels to respond to growth conditions.
    Title: The MiaA tRNA modification enzyme is necessary for robust RpoS expression in Escherichia coli.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables tRNA dimethylallyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables tRNA dimethylallyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables tRNA dimethylallyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity, transferring alkyl or aryl (other than methyl) groups IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within cellular response to heat IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within cellular response to heat IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within regulation of cytoplasmic translation in response to stress IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in tRNA modification IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within tRNA modification IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in tRNA processing IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
tRNA dimethylallyltransferase
NP_418592.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4399252..4400202
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418592.1 tRNA dimethylallyltransferase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418592.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A485JKD5, A0A775ZGC0
    Conserved Domains (1) summary
    PRK00091
    Location:7 → 313
    miaA; tRNA delta(2)-isopentenylpyrophosphate transferase; Reviewed
Nucleotide Protein
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Gene LinkOut

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