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hflX [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948688, updated on 24-Aug-2024

Summary

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Official Symbol
hflX
Primary source
ASAP:ABE-0013661
Locus tag
b4173
See related
ECOCYC:EG10437
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4169; hflA; hslX
Summary
HflX has both GTPase and ATPase activities (Jain, 2009; Dutta, 2009). [More information is available at EcoGene: EG10437]. HflX has a GTP-binding/GTPase region , and it may be involved in regulation of HflC and HflK activity . [More information is available at EcoCyc: EG10437].
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Genomic context

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Sequence:
NC_000913.3 (4400672..4401952)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA mismatch repair protein MutL Neighboring gene tRNA dimethylallyltransferase Neighboring gene RNA-binding protein Hfq Neighboring gene regulator of FtsH protease Neighboring gene regulator of FtsH protease

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. HflX protein protects Escherichia coli from manganese stress. Sengupta S, et al. J Biosci, 2018 Dec. PMID 30541959
  2. The universally conserved GTPase HflX is an RNA helicase that restores heat-damaged Escherichia coli ribosomes. Dey S, et al. J Cell Biol, 2018 Jul 2. PMID 29930203, Free PMC Article
  3. Characterization of the autophosphorylation property of HflX, a ribosome-binding GTPase from Escherichia coli. Ghosh A, et al. FEBS Open Bio, 2016 Jul. PMID 27398305, Free PMC Article
  4. The conserved GTPase HflX is a ribosome splitting factor that binds to the E-site of the bacterial ribosome. Coatham ML, et al. Nucleic Acids Res, 2016 Feb 29. PMID 26733579, Free PMC Article
  5. HflX is a ribosome-splitting factor rescuing stalled ribosomes under stress conditions. Zhang Y, et al. Nat Struct Mol Biol, 2015 Nov. PMID 26458047

See all (30) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. cryo-electron microscopy structure of the 50S-HflX complex in the presence of nonhydrolyzable analogues of ATP and guanosine triphosphate hints at a mode of action for the RNA helicase and suggests the linker helical domain may have a determinant role in RNA unwinding. Heat stress results in inactivation of the ribosome, and we show that HflX can restore heat-damaged ribosomes and improve cell survival.
    Title: The universally conserved GTPase HflX is an RNA helicase that restores heat-damaged Escherichia coli ribosomes.
  2. HflX protein protects Escherichia coli from manganese stress.
    Title: HflX protein protects Escherichia coli from manganese stress.
  3. Furthermore, we provide evidence that the bound state of the peptidyl transferase centre is able to regulate the GTPase activity of E-site bound HflX
    Title: The conserved GTPase HflX is a ribosome splitting factor that binds to the E-site of the bacterial ribosome.
  4. These results suggest a primary role of HflX in rescuing translationally arrested ribosomes under stress conditions.
    Title: HflX is a ribosome-splitting factor rescuing stalled ribosomes under stress conditions.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables GTP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables GTP binding IEA
Inferred from Electronic Annotation
more info
  
enables GTPase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables GTPase activity IEA
Inferred from Electronic Annotation
more info
  
enables guanosine tetraphosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables rRNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables ribosomal large subunit binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables ribosome binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ribosome binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within rescue of stalled ribosome IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within response to heat IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within response to heat IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within ribosome disassembly IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within ribosome disassembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
NOT acts_upstream_of_or_within viral latency IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
ribosome rescue factor HflX
NP_418594.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4400672..4401952
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418594.1 ribosome rescue factor HflX [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418594.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9AM10
    Conserved Domains (1) summary
    PRK11058
    Location:1 → 426
    PRK11058; GTPase HflX; Provisional
Nucleotide Protein
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