mreB [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: mreB
Primary source: ECOCYC:EG10608
Locus tag: b3251
See related: ASAP:ABE-0010661
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK3239; envB; mon; mreB_1; rodY
Summary: Overexpression causes abnormal biofilm architecture, reduces motility and inhibits cell division. [More information is available at EcoGene: EG10608]. The mre genes are responsible for rod shape and mecillinam sensitivity in Escherichia coli . [More information is available at EcoCyc: EG10608].
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Genomic context

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Sequence:: NC_000913.3 (3400044..3401087, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Bimetallic gold-silver nanoparticles mediate bacterial killing by disrupting the actin cytoskeleton MreB.
Title: Bimetallic gold-silver nanoparticles mediate bacterial killing by disrupting the actin cytoskeleton MreB.
MreBA125V and MreBA174T mutant cells were thinner and thicker than WT cells, and MreBA125V and MreBA174T rotated faster and slower than WT, respectively.
Title: Relation between rotation of MreB actin and cell width of Escherichia coli.
Studies indicate the function of dynamic cytoskeletal protein MreB to generate an integrated model of rod-shaped wall construction [Review].
Title: How to Build a Bacterial Cell: MreB as the Foreman of E. coli Construction.
MreB mutants alter the bending properties of MreB filaments in molecular dynamics simulations similar to RodZ binding, and these mutants rescue rod-like shape in the absence of RodZ alone or in combination with wild-type MreB.
Title: RodZ modulates geometric localization of the bacterial actin MreB to regulate cell shape.
The data suggest that RodZ promotes the assembly of geometrically-localized MreB polymers that lead to the growth of uniform E. coli cylinders.
Title: MreB polymers and curvature localization are enhanced by RodZ and predict E. coli's cylindrical uniformity.
MreB is not recruited to the FtsZ ring in secA mutant cells, contributing to division arrest and cell filamentation.. Thus, when we reroute RodZ, MreB membrane-anchor, by fusing it to a SecA-independent integral membrane protein and overproducing it, MreB localization is restored and the defect in cell division is corrected.
Title: The bacterial Sec system is required for the organization and function of the MreB cytoskeleton.
Although several other protein modulators are known to target FtsZ, the CbtA-interacting surface we identify represents a novel inhibitory target. Our findings establish CbtA as a dual function toxin that inhibits both cell division and cell elongation via direct and independent interactions with FtsZ and MreB.
Title: CbtA toxin of Escherichia coli inhibits cell division and cell elongation via direct and independent interactions with FtsZ and MreB.
Results demonstrate that the physical properties of MreB filaments are important for shape control and support a model in which MreB organizes the cell wall growth machinery to produce a chiral cell wall structure and dictate cell diameter
Title: MreB Orientation Correlates with Cell Diameter in Escherichia coli.
E75, R78 and D82 of FtsZ are key residues for FtsZ self-assembly and FtsZ-MreB interaction.
Title: [E75, R78 and D82 of Escherichia coli FtsZ are key residues for FtsZ cellular self-assembly and FtsZ-MreB interaction].
Data show that actin homolog MreB rotates because cytoplasmic MreB filaments are coupled to periplasmic cell wall synthesis through the transmembrane protein RodZ, which acts as a transmembrane linker.
Title: RodZ links MreB to cell wall synthesis to mediate MreB rotation and robust morphogenesis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within FtsZ-dependent cytokinesis	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within FtsZ-dependent cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell morphogenesis	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of cell division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peptidoglycan biosynthetic process	NAS Non-traceable Author Statement more info	PubMed
involved_in regulation of cell shape	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of cell shape	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell shape	NAS Non-traceable Author Statement more info	PubMed
acts_upstream_of_or_within regulation of chromosome segregation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within regulation of chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	NAS Non-traceable Author Statement more info	PubMed
located_in polymeric cytoskeletal fiber	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: dynamic cytoskeletal protein MreB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

3400044..3401087 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_417717.2 dynamic cytoskeletal protein MreB [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_417717.2

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A5B9AS38

Conserved Domains (1) summary

PRK13927
Location:6 → 345

PRK13927; rod shape-determining protein MreB; Provisional