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nrfA cytochrome c552 nitrite reductase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948571, updated on 25-Jun-2025
Official Symbol
nrfA
Official Full Name
cytochrome c552 nitrite reductase
Primary source
ECOCYC:EG11781
Locus tag
b4070
See related
ASAP:ABE-0013342
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4063
Summary
The gene nrfA is the structural gene for cytochrome C552, which functions as a formate-dependent nitrite reductase. [More information is available at EcoCyc: EG11781].
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Sequence:
NC_000913.3 (4287764..4289200)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene acetyl-CoA synthetase (AMP-forming) Neighboring gene protein YtcB Neighboring gene periplasmic nitrite reductase penta-heme c-type cytochrome Neighboring gene putative menaquinol-cytochrome c reductase 4Fe-4S subunit Neighboring gene putative menaquinol-cytochrome c reductase subunit NrfD

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Kinetic and thermodynamic resolution of the interactions between sulfite and the pentahaem cytochrome NrfA from Escherichia coli. Kemp GL, et al. Biochem J, 2010 Oct 1. PMID 20629638
  2. Purification of a hexaheme cytochrome c552 from Escherichia coli K 12 and its properties as a nitrite reductase. Kajie S, et al. Eur J Biochem, 1986 Jan 15. PMID 3002798
  3. Spectroelectrochemical characterization of a pentaheme cytochrome in solution and as electrocatalytically active films on nanocrystalline metal-oxide electrodes. Marritt SJ, et al. J Am Chem Soc, 2008 Jul 9. PMID 18549208
  4. Escherichia coli cytochrome c nitrite reductase NrfA. Clarke TA, et al. Methods Enzymol, 2008. PMID 18433623
  5. Studies on soluble cytochromes in Enterobacteriaceae. I. Detection, purification, and properties of cytochrome c-552 in anaerobically grown cells. Fujita T. J Biochem, 1966 Aug. PMID 5338848

See all (40) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Elucidating Electron Storage and Distribution within the Pentaheme Scaffold of Cytochrome c Nitrite Reductase (NrfA).
    Title: Elucidating Electron Storage and Distribution within the Pentaheme Scaffold of Cytochrome c Nitrite Reductase (NrfA).
  2. Kinetic and thermodynamic resolution of the interactions between sulfite and the pentahaem cytochrome NrfA from Escherichia coli
    Title: Kinetic and thermodynamic resolution of the interactions between sulfite and the pentahaem cytochrome NrfA from Escherichia coli.
  3. Activation of the Escherichia coli K-12 nrf promoter is downregulated by the nitric oxide-sensitive NsrR protein together with the nucleoid-associated protein IHF.
    Title: Down-regulation of the Escherichia coli K-12 nrf promoter by binding of the NsrR nitric oxide-sensing transcription repressor to an upstream site.
  4. Spectroelectrochemical studies of E. coli NrfA have provided unprecedented resolution of its thermodynamic properties.
    Title: Spectroelectrochemical characterization of a pentaheme cytochrome in solution and as electrocatalytically active films on nanocrystalline metal-oxide electrodes.
  5. Method used to assay Nrf-catalyzed NO reduction in whole cells of E. coli and the procedures for preparing highly purified NrfA suitable for use in kinetic, spectroscopic, voltammetric, and crystallization studies.
    Title: Escherichia coli cytochrome c nitrite reductase NrfA.
  6. N-terminus verified by Edman degradation on mature peptide
    Title: Regulation and sequence of the structural gene for cytochrome c552 from Escherichia coli: not a hexahaem but a 50 kDa tetrahaem nitrite reductase.
  7. Protein-protein interaction studies suggest NrfA and NrfB can form a 20-haem NrfA2-NrfB2 heterotetrameric complex.
    Title: The crystal structure of the pentahaem c-type cytochrome NrfB and characterization of its solution-state interaction with the pentahaem nitrite reductase NrfA.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables heme binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables heme binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables nitric oxide reductase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables nitrite reductase (cytochrome, ammonia-forming) activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables nitrite reductase (cytochrome, ammonia-forming) activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in anaerobic electron transport chain IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within anaerobic electron transport chain IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in anaerobic respiration IEA
Inferred from Electronic Annotation
more info
  
involved_in nitrate assimilation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in outer membrane-bounded periplasmic space IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in outer membrane-bounded periplasmic space IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
cytochrome c552 nitrite reductase

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4287764..4289200
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418494.1 cytochrome c552 nitrite reductase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418494.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A403CWJ2, A0AAP3EHY4
    Conserved Domains (1) summary
    PRK11125
    Location:4 → 478
    nrfA; ammonia-forming cytochrome c nitrite reductase
Nucleotide Protein
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