dnaB replicative DNA helicase [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Gene symbol: dnaB
Gene description: replicative DNA helicase
Primary source: EcoGene:EG10236
Locus tag: b4052
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK4044; groP; grpA; grpD; JW4012
Summary: The dnaB gene is essential. [More information is available at EcoGene: EG10236]. DnaB, the replicative DNA helicase, processively unwinds DNA at replication forks in advance of DNA polymerase. [More information is available at EcoCyc: EG10236].

Genomic context

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Sequence:: NC_000913.3 (4264314..4265729)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

Thus, imbalances in levels of the replicative helicase and helicase loader can inhibit replication both via inhibition of DnaB6-DnaC6 complex formation with excess DnaB, as shown here, and promotion of formation of DnaB6-DnaC6 complexes with excess DnaC.
Title: Overexpression of the Replicative Helicase in Escherichia coli Inhibits Replication Initiation and Replication Fork Reloading.
Data demonstrate that DnaC opens DnaB by a mechanism akin to that of polymerase clamp loaders and indicate that bacterial replicative helicases, like their eukaryotic counterparts, possess autoregulatory elements that influence how hexameric motor domains are loaded onto and unwind DNA.
Title: The bacterial DnaC helicase loader is a DnaB ring breaker.
The authors show that the binding of DnaA on the NrdAB promoter can either activate or repress transcription as a function of its concentration and its nucleotide-bound state.
Title: DnaA-ATP acts as a molecular switch to control levels of ribonucleotide reductase expression in Escherichia coli.
HpDnaB differs greatly from E.coli DnaB despite both being gram negative bacteria
Title: Three-dimensional structure of N-terminal domain of DnaB helicase and helicase-primase interactions in Helicobacter pylori.
When coupled to the replicative helicase DnaB, Pol III mediates leading-strand synthesis with a processivity of 10.5 kilobases (kb), eight-fold higher than that by Pol III alone.
Title: Single-molecule studies of fork dynamics in Escherichia coli DNA replication.
N-terminus verified by Edman degradation on mature peptide
Title: Nucleotide sequence of dnaB and the primary structure of the dnaB protein from Escherichia coli.
These findings suggest a model for DnaA homomultimer formation and DnaB helicase loading on oriC.
Title: Structure and function of DnaA N-terminal domains: specific sites and mechanisms in inter-DnaA interaction and in DnaB helicase loading on oriC.
there is a specific requirement for DnaB in P1 plasmid maintenance in addition to the general requirement for DnaB as the replicative helicase during elongation.
Title: Identification and characterization of a novel allele of Escherichia coli dnaB helicase that compromises the stability of plasmid P1.
the connecting helix contributes necessary structural flexibility in the primase-helicase complex at replication forks.
Title: Crystal and solution structures of the helicase-binding domain of Escherichia coli primase.
These results suggest that in the presence of ATP in vivo, the DnaB6 x DnaC6 complex should be more efficient in binding DNA as well as in loading DnaB onto the ssDNA than DnaB helicase itself.
Title: Quantitative analysis of binding of single-stranded DNA by Escherichia coli DnaB helicase and the DnaB x DnaC complex.

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Pathways from BioSystems

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DNA replication, organism-specific biosystem (from KEGG)
DNA replication, organism-specific biosystemA complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (D...
DNA replication, conserved biosystem (from KEGG)
DNA replication, conserved biosystemA complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (D...

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by GO

Function	Evidence Code	Pubs
ATP binding	IEA Inferred from Electronic Annotation more info
DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info	PubMed
DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
DNA helicase activity	IEA Inferred from Electronic Annotation more info
helicase activity	IDA Inferred from Direct Assay more info	PubMed
identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
DNA replication	IEA Inferred from Electronic Annotation more info
DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
DNA unwinding involved in DNA replication	IBA Inferred from Biological aspect of Ancestor more info	PubMed
DNA unwinding involved in DNA replication	IDA Inferred from Direct Assay more info	PubMed
response to ionizing radiation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
cytosol	IBA Inferred from Biological aspect of Ancestor more info	PubMed
cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Names: replicative DNA helicase

NP_418476.1

EC 3.6.1.-

replicative DNA helicase; part of primosome

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

4264314..4265729

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_418476.1 replicative DNA helicase [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_418476.1

Status: PROVISIONAL

UniProtKB/Swiss-Prot

P0ACB0

Conserved Domains (1) summary

PRK08006
Location:1 → 471

PRK08006; replicative DNA helicase; Provisional