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lexA DNA-binding transcriptional repressor LexA [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948544, updated on 26-Mar-2025
Official Symbol
lexA
Official Full Name
DNA-binding transcriptional repressor LexA
Primary source
ECOCYC:EG10533
Locus tag
b4043
See related
ASAP:ABE-0013241
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4035; exrA; spr; tsl; umuA
Summary
LexA autocleavage, stimulated by RecA, of the first 84 aa of LexA removes the DNA binding region and is required to activate the SOS response. [More information is available at EcoGene: EG10533]. LexA is a protein that belongs to the LexA family . [More information is available at EcoCyc: EG10533].
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Sequence:
NC_000913.3 (4257115..4257723)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene glycerol-3-phosphate 1-O-acyltransferase Neighboring gene diacylglycerol kinase Neighboring gene DNA damage-inducible protein F Neighboring gene putative stress response protein YjbJ

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Expression of lexA targeted ribozyme in Escherichia coli BL-21 (DE3) cells. Yadava RS, et al. Mol Cell Biochem, 2005 Mar. PMID 15881671
  2. Mutant LexA proteins with specific defects in autodigestion. Shepley DP, et al. Proc Natl Acad Sci U S A, 1996 Oct 15. PMID 8876169, Free PMC Article
  3. sfi-independent filamentation in Escherichia coli Is lexA dependent and requires DNA damage for induction. Hill TM, et al. J Bacteriol, 1997 Mar. PMID 9068638, Free PMC Article
  4. LexA repressor forms stable dimers in solution. The role of specific dna in tightening protein-protein interactions. Mohana-Borges R, et al. J Biol Chem, 2000 Feb 18. PMID 10671501
  5. Model of a LexA repressor dimer bound to recA operator. Chattopadhyaya R, et al. J Biomol Struct Dyn, 2000 Oct. PMID 11089640

See all (131) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Non-canonical LexA proteins regulate the SOS response in the Bacteroidetes.
    Title: Non-canonical LexA proteins regulate the SOS response in the Bacteroidetes.
  2. Single-molecule imaging of LexA degradation in Escherichia coli elucidates regulatory mechanisms and heterogeneity of the SOS response.
    Title: Single-molecule imaging of LexA degradation in Escherichia coli elucidates regulatory mechanisms and heterogeneity of the SOS response.
  3. The Parameter-Fitness Landscape of lexA Autoregulation in Escherichia coli.
    Title: The Parameter-Fitness Landscape of lexA Autoregulation in Escherichia coli.
  4. Two components of DNA replication-dependent LexA cleavage.
    Title: Two components of DNA replication-dependent LexA cleavage.
  5. YbiB from Escherichia coli, the defining member of the novel Trpd2 family of prokaryotic DNA-binding proteins, forms an operon with the DNA helicase gene dinG and is under LexA control.
    Title: YbiB from Escherichia coli, the Defining Member of the Novel TrpD2 Family of Prokaryotic DNA-binding Proteins.
  6. Like the self-cleaving serine proteases LexA and UmuD in Escherichia coli, UmuDAb required RecA for cleavage.
    Title: The Acinetobacter regulatory UmuDAb protein cleaves in response to DNA damage with chimeric LexA/UmuD characteristics.
  7. These results suggest that, IscR stabilizes LexA at the cka promoter after DNA damage thus, preventing its cleavage and inactivation, and this cooperation ensures that suicidal colicin K production is switched on only as a last resort.
    Title: Double locking of an Escherichia coli promoter by two repressors prevents premature colicin expression and cell lysis.
  8. These results reveal specific amino acid determinants of the RecA-LexA interaction and suggest that LexA binds RecA and RecAi+6 at a composite site on the RecA filament, which could explain the role of the active filament during LexA cleavage.
    Title: Separation of recombination and SOS response in Escherichia coli RecA suggests LexA interaction sites.
  9. The conformational flexibility of unbound LexA is the key element in establishing a co-ordinated SOS response.
    Title: Interconversion between bound and free conformations of LexA orchestrates the bacterial SOS response.
  10. LexA regulated genes exhibit phenotypic heterogeneity as high level expression is observed in only a small subpopulation.
    Title: Genes regulated by the Escherichia coli SOS repressor LexA exhibit heterogeneous expression.
Submit: New GeneRIF Correction See all GeneRIFs (15)
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA-binding transcription repressor activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA-binding transcription repressor activity IPI
Inferred from Physical Interaction
more info
 PubMed 
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables sequence-specific DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables serine-type endopeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables transcription cis-regulatory region binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA damage response IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within DNA damage response IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA replication IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within DNA-templated transcription IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within DNA-templated transcription IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in SOS response IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in SOS response IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within SOS response IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of DNA-templated transcription EXP
Inferred from Experiment
more info
 PubMed 
involved_in negative regulation of DNA-templated transcription IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in negative regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of DNA-templated transcription IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within negative regulation of DNA-templated transcription IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in proteolysis IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of protein-DNA complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of protein-DNA complex IPI
Inferred from Physical Interaction
more info
 PubMed 
Preferred Names
DNA-binding transcriptional repressor LexA

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4257115..4257723
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418467.1 DNA-binding transcriptional repressor LexA [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418467.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8S0FHY8
    Conserved Domains (1) summary
    PRK00215
    Location:3 → 199
    PRK00215; transcriptional repressor LexA
Nucleotide Protein
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