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dgkA diacylglycerol kinase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948543, updated on 30-Jul-2025
Official Symbol
dgkA
Official Full Name
diacylglycerol kinase
Primary source
ECOCYC:EG10224
Locus tag
b4042
See related
ASAP:ABE-0013235
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4034
Summary
DgkA is an integral membrane protein. [More information is available at EcoGene: EG10224]. Diacylglycerol kinase is the smallest known kinase. [More information is available at EcoCyc: EG10224].
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Sequence:
NC_000913.3 (4256637..4257005)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 4-hydroxybenzoate octaprenyltransferase Neighboring gene glycerol-3-phosphate 1-O-acyltransferase Neighboring gene DNA-binding transcriptional repressor LexA Neighboring gene DNA damage-inducible protein F

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Appearance of monoglyceride and triglyceride in the cell envelope of Escherichia coli mutants defective in diglyceride kinase. Rotering H, et al. J Biol Chem, 1983 Jul 10. PMID 6305970
  2. Dynamic pictures of membrane proteins in two-dimensional crystal, lipid bilayer and detergent as revealed by site-directed solid-state 13C NMR. Saitô H. Chem Phys Lipids, 2004 Nov. PMID 15530452
  3. NMR assignments for a helical 40 kDa membrane protein. Oxenoid K, et al. J Am Chem Soc, 2004 Apr 28. PMID 15099070
  4. Destabilizing mutations promote membrane protein misfolding. Nagy JK, et al. Biochemistry, 2004 Jan 13. PMID 14705927
  5. THE BIOSYNTHESIS OF PHOSPHATIDIC ACID AND LYSOPHOSPHATIDIC ACID BY GLYCERIDE PHOSPHOKINASE PATHWAYS IN ESCHERICHIA COLI. PIERINGER RA, et al. J Biol Chem, 1965 Jul. PMID 14342303

See all (43) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The active site of dgkA including glutamates and an aspartate plays roles in substrate binding and catalysis.
    Title: Ternary structure reveals mechanism of a membrane diacylglycerol kinase.
  2. PlsB activation by RpoE is concomitant with a reduced DgkA amount.
    Title: Antagonistic regulation of dgkA and plsB genes of phospholipid synthesis by multiple stress responses in Escherichia coli.
  3. Magic angle spinning NMR allows simultaneous & direct detection of both ATP hydrolysis and diacylglycerol phosphorylation for DGK; data yield conclusions about intersubunit cooperativity, reaction stoichiometries and phosphoryl transfer mechanism.
    Title: Interfacial enzyme kinetics of a membrane bound kinase analyzed by real-time MAS-NMR.
  4. determination of the three-dimensional structure of the diacylglycerol kinase homotrimer with the use of solution nuclear magnetic resonance
    Title: Solution nuclear magnetic resonance structure of membrane-integral diacylglycerol kinase.
  5. Diacylglycerol kinase from Escherichia coli contains three transmembrane alpha-helices per monomer. We used fluorescence techniques to define region of first transmembrane helix that spans the hydrophobic core of the reconstituted membranes.
    Title: A fluorescence method to define transmembrane alpha-helices in membrane proteins: studies with bacterial diacylglycerol kinase.
  6. N-terminus verified by Edman degradation on mature peptide
    Title: sn-1,2-Diacylglycerol kinase of Escherichia coli. Purification, reconstitution, and partial amino- and carboxyl-terminal analysis.
  7. Results describe the mechanism of folding and insertion into lipid bilayers of Escherichia coli diacylglycerol kinase from a partially denatured state in urea.
    Title: Insertion kinetics of a denatured alpha helical membrane protein into phospholipid bilayer vesicles.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP-dependent diacylglycerol kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ATP-dependent diacylglycerol kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables lipid kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables metal ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in lipid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in lipid metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in phosphatidic acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within phosphatidic acid biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in phospholipid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within response to UV IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
diacylglycerol kinase
NP_418466.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4256637..4257005
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418466.1 diacylglycerol kinase

    See identical proteins and their annotated locations for NP_418466.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A5B9AQS0
    Conserved Domains (1) summary
    COG0818
    Location:1 → 121
    DgkA; Diacylglycerol kinase [Lipid transport and metabolism]
Nucleotide Protein
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