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metH cobalamin-dependent methionine synthase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948522, updated on 30-Jul-2025
Official Symbol
metH
Official Full Name
cobalamin-dependent methionine synthase
Primary source
ECOCYC:EG10587
Locus tag
b4019
See related
ASAP:ABE-0013140
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK4011
Summary
[More information is available at EcoCyc: EG10587].
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Sequence:
NC_000913.3 (4223828..4227511)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene isocitrate dehydrogenase kinase/phosphatase Neighboring gene ankyrin-like regulator Neighboring gene DNA-binding transcriptional repressor IclR Neighboring gene putative inorganic phosphate export protein YjbB Neighboring gene peptidase E

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Cobalamin-dependent methionine synthase from Escherichia coli: involvement of zinc in homocysteine activation. Goulding CW, et al. Biochemistry, 1997 Dec 16. PMID 9398304
  2. Mutations affecting regulation of methionine biosynthetic genes isolated by use of met-lac fusions. Mulligan JT, et al. J Bacteriol, 1982 Aug. PMID 6284705, Free PMC Article
  3. N5-methyltetrahydrofolate-homocysteine transmethylase. Role of S-adenosylmethionine in vitamin B12-dependent methionine synthesis. Taylor RT, et al. J Biol Chem, 1967 Apr 10. PMID 5337044
  4. Assignment of enzymatic function to specific protein regions of cobalamin-dependent methionine synthase from Escherichia coli. Drummond JT, et al. Biochemistry, 1993 Sep 14. PMID 8369297
  5. A synthetic module for the metH gene permits facile mutagenesis of the cobalamin-binding region of Escherichia coli methionine synthase: initial characterization of seven mutant proteins. Amaratunga M, et al. Biochemistry, 1996 Feb 20. PMID 8652589

See all (46) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. describe an x-ray structure of the mutant fragment in the reactivation conformation
    Title: A disulfide-stabilized conformer of methionine synthase reveals an unexpected role for the histidine ligand of the cobalamin cofactor.
  2. N-terminus verified by Edman degradation on mature peptide
    Title: Cloning and sequence analysis of the Escherichia coli metH gene encoding cobalamin-dependent methionine synthase and isolation of a tryptic fragment containing the cobalamin-binding domain.
Submit: New GeneRIF Correction

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables cobalamin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables cobalamin binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables methionine synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables methionine synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables methionine synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in L-methionine salvage IEA
Inferred from Electronic Annotation
more info
  
involved_in amino acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in homocysteine metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in homocysteine metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within methionine biosynthetic process EXP
Inferred from Experiment
more info
 PubMed 
involved_in methionine biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in methionine biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in methionine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within methionine biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in methylation IEA
Inferred from Electronic Annotation
more info
  
involved_in pteridine-containing compound metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in tetrahydrofolate interconversion IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in tetrahydrofolate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
cobalamin-dependent methionine synthase
NP_418443.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4223828..4227511
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418443.1 cobalamin-dependent methionine synthase

    See identical proteins and their annotated locations for NP_418443.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A8T6PZC3
    Conserved Domains (1) summary
    PRK09490
    Location:1 → 1223
    metH; B12-dependent methionine synthase; Provisional
Nucleotide Protein
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