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thiE thiamine phosphate synthase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948491, updated on 17-Dec-2024
Official Symbol
thiE
Official Full Name
thiamine phosphate synthase
Primary source
ECOCYC:EG11586
Locus tag
b3993
See related
ASAP:ABE-0013055
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3985; thiA
Summary
The ThiE sequence has been confirmed using MS/MS: the measured mass of the intact protein is 23014.8 indicating that the initial Met is not clipped (Kelleher, 1998). [More information is available at EcoGene: EG11586]. Thiamine phosphate synthase combines 4-methyl-5-(-hydroxyethyl)thiazole phosphate and 4-amino-5-hydroxymethyl-2-methylpyrimidine-pyrophosphate to generate thiamine phosphate . [More information is available at EcoCyc: EG11586].
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Sequence:
NC_000913.3 (4193569..4194204, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 2-iminoacetate synthase Neighboring gene 1-deoxy-D-xylulose 5-phosphate:thiol sulfurtransferase Neighboring gene sulfur carrier protein ThiS Neighboring gene sulfur carrier protein ThiS adenylyltransferase Neighboring gene phosphomethylpyrimidine synthase Neighboring gene regulator of sigma D

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Purification and properties of thiaminephosphate pyrophosphorylase of Escherichia coli. Kayama Y, et al. Arch Biochem Biophys, 1973 Sep. PMID 4580841
  2. Thiamine phosphate pyrophosphorylase. Kawasaki T. Methods Enzymol, 1979. PMID 374983
  3. Efficient sequence analysis of the six gene products (7-74 kDa) from the Escherichia coli thiamin biosynthetic operon by tandem high-resolution mass spectrometry. Kelleher NL, et al. Protein Sci, 1998 Aug. PMID 10082377, Free PMC Article
  4. A genetic screen for the identification of thiamin metabolic genes. Lawhorn BG, et al. J Biol Chem, 2004 Oct 15. PMID 15292217
  5. The mechanism of action of bacimethrin, a naturally occurring thiamin antimetabolite. Reddick JJ, et al. Bioorg Med Chem Lett, 2001 Sep 3. PMID 11527707

See all (12) citations in PubMed

GeneRIFs: Gene References Into Functions

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables magnesium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables magnesium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables thiamine-phosphate diphosphorylase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables thiamine-phosphate diphosphorylase activity IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in thiamine biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within thiamine biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in thiamine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in thiamine diphosphate biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
Preferred Names
thiamine phosphate synthase
NP_418421.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4193569..4194204 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418421.1 thiamine phosphate synthase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418421.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A1X3J9J8
    Conserved Domains (1) summary
    PRK03512
    Location:1 → 211
    PRK03512; thiamine phosphate synthase
Nucleotide Protein
Heading Accession and Version

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