emrE multidrug/betaine/choline efflux transporter EmrE [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: emrE
Official Full Name: multidrug/betaine/choline efflux transporter EmrE
Primary source: ECOCYC:EG10629
Locus tag: b0543
See related: ASAP:ABE-0001863
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: eb; ECK0534; mvrC
Summary: EmrE defunct PDB structures 1S7B and 2F2M, and any conclusions derived from these structures, have been retracted due to a software data inversion error (Ma, 2007; Chang, 2007). [More information is available at EcoGene: EG10629]. EmrE is a multidrug efflux protein with a broad substrate specificity. [More information is available at EcoCyc: EG10629].
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Genomic context

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Sequence:: NC_000913.3 (568315..568647)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Charge neutralization of the active site glutamates does not limit substrate binding and transport by small multidrug resistance transporter EmrE.
Title: Charge neutralization of the active site glutamates does not limit substrate binding and transport by small multidrug resistance transporter EmrE.
Small multidrug resistance protein EmrE phenotypically associates with OmpW, DcrB and YggM for osmotic stress protection by betaine in Escherichia coli.
Title: Small multidrug resistance protein EmrE phenotypically associates with OmpW, DcrB and YggM for osmotic stress protection by betaine in Escherichia coli.
Activating alternative transport modes in a multidrug resistance efflux pump to confer chemical susceptibility.
Title: Activating alternative transport modes in a multidrug resistance efflux pump to confer chemical susceptibility.
Cotranslational folding and assembly of the dimeric Escherichia coli inner membrane protein EmrE.
Title: Cotranslational folding and assembly of the dimeric Escherichia coli inner membrane protein EmrE.
High-pH structure of EmrE reveals the mechanism of proton-coupled substrate transport.
Title: High-pH structure of EmrE reveals the mechanism of proton-coupled substrate transport.
Molecular mechanism of proton-coupled ligand translocation by the bacterial efflux pump EmrE.
Title: Molecular mechanism of proton-coupled ligand translocation by the bacterial efflux pump EmrE.
Inducing conformational preference of the membrane protein transporter EmrE through conservative mutations.
Title: Inducing conformational preference of the membrane protein transporter EmrE through conservative mutations.
Few conserved amino acids in the small multidrug resistance transporter EmrE influence drug polyselectivity.
Title: Few Conserved Amino Acids in the Small Multidrug Resistance Transporter EmrE Influence Drug Polyselectivity.
Optimal positions of the peptide staple were determined using free-energy simulations of peptide binding to monomeric EmrE Three of the four top-scoring peptides selected for experimental testing resulted in significant inhibition of proton-driven ethidium efflux in live cells without nonspecific toxicity. The approach described here is expected to be of general use for the design of peptide therapeutics.
Title: Structure of the EmrE multidrug transporter and its use for inhibitor peptide design.
Not only can EmrE export diverse drug substrates, it can couple antiport of a drug to either one or two protons, performing both electrogenic and electroneutral transport of a single substrate.
Title: New free-exchange model of EmrE transport.

Submit: New GeneRIF Correction See all GeneRIFs (39)

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables amino-acid betaine transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables amino-acid betaine transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables antiporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables antiporter activity	IDA Inferred from Direct Assay more info	PubMed
enables antiporter activity	IEA Inferred from Electronic Annotation more info
enables choline transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables choline transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables transmembrane transporter activity	IEA Inferred from Electronic Annotation more info
enables xenobiotic transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA damage response	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to xenobiotic stimulus	IDA Inferred from Direct Assay more info	PubMed
involved_in choline transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in choline transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glycine betaine transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in glycine betaine transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to osmotic stress	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to xenobiotic stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in xenobiotic detoxification by transmembrane export across the plasma membrane	IDA Inferred from Direct Assay more info	PubMed
involved_in xenobiotic detoxification by transmembrane export across the plasma membrane	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within xenobiotic metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in xenobiotic transport	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISM Inferred from Sequence Model more info	PubMed
located_in plasma membrane	RCA inferred from Reviewed Computational Analysis more info	PubMed

General protein information

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Preferred Names: multidrug/betaine/choline efflux transporter EmrE

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

568315..568647

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_415075.1 multidrug/betaine/choline efflux transporter EmrE

See identical proteins and their annotated locations for NP_415075.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0H3EFV7

Conserved Domains (1) summary

PRK09541
Location:1 → 110

emrE; EmrE family multidrug efflux SMR transporter