U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

metJ DNA-binding transcriptional repressor MetJ [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948435, updated on 23-Apr-2026
Official Symbol
metJ
Official Full Name
DNA-binding transcriptional repressor MetJ
Primary source
ECOCYC:EG10588
Locus tag
b3938
See related
ASAP:ABE-0012874
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3930
Summary
Upregulated by GSNO in defined medium (aerobic). [More information is available at EcoGene: EG10588].
Try the new Gene page
Try the new Transcripts and proteins table
Sequence:
NC_000913.3 (4128078..4128395, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 50S ribosomal subunit protein L31 Neighboring gene putative lipid-binding hydrolase YiiX Neighboring gene O-succinylhomoserine(thiol)-lyase/O-succinylhomoserine lyase Neighboring gene fused aspartate kinase/homoserine dehydrogenase 2

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. The Escherichia coli K-12 metJ193 allele contains a point mutation which alters the hydrophobic pocket responsible for in vitro binding of S-adenosylmethionine: effects on cell growth and induction of met regulon expression. Collier CD, et al. J Bacteriol, 1990 Jul. PMID 2141834, Free PMC Article
  2. Conformational model for binding site recognition by the E.coli MetJ transcription factor. Liu R, et al. Bioinformatics, 2001 Jul. PMID 11448880
  3. Mechanism of L-methionine overproduction by Escherichia coli: the replacement of Ser-54 by Asn in the MetJ protein causes the derepression of L-methionine biosynthetic enzymes. Nakamori S, et al. Appl Microbiol Biotechnol, 1999 Aug. PMID 10499257
  4. Kinetic analysis of operator binding by the E. coli methionine repressor highlights the role(s) of electrostatic interactions. Lawrenson ID, et al. FEBS Lett, 2004 Apr 23. PMID 15094055
  5. Cloning of the methionine regulatory gene, metJ, of Escherichia coli K12 and identification of its product. Smith AA, et al. J Biol Chem, 1984 Nov 25. PMID 6389545

See all (46) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. We found that potentiating mutations in metJ increase expression of metA, which encodes the first step of methionine biosynthesis. This increase in expression is required for the previously identified actualizing mutations in metA to generate cooperation.
    Title: Identification of the potentiating mutations and synergistic epistasis that enabled the evolution of inter-species cooperation.
  2. N-terminus verified by Edman degradation on mature peptide
    Title: Structure and autoregulation of the metJ regulatory gene in Escherichia coli.
  3. Transcript analysis reveals an extended regulon and the importance of protein-protein co-operativity for the Escherichia coli methionine repressor
    Title: Transcript analysis reveals an extended regulon and the importance of protein-protein co-operativity for the Escherichia coli methionine repressor.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables DNA-binding transcription factor activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in L-methionine metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
DNA-binding transcriptional repressor MetJ

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4128078..4128395 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418373.1 DNA-binding transcriptional repressor MetJ

    See identical proteins and their annotated locations for NP_418373.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A1X3ITW5, A0AAJ3NUW2, A0AAN3M6I8
    Conserved Domains (1) summary
    PRK05264
    Location:1 → 105
    PRK05264; met regulon transcriptional regulator MetJ
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.