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glpK glycerol kinase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948423, updated on 26-Mar-2025
Official Symbol
glpK
Official Full Name
glycerol kinase
Primary source
ECOCYC:EG10398
Locus tag
b3926
See related
ASAP:ABE-0012825
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3918
Summary
The enzyme can undergo a reversible subunit dissociation between tetramer and dimer . [More information is available at EcoCyc: EG10398].
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Sequence:
NC_000913.3 (4115714..4117222, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene flavodoxin/ferredoxin-NADP(+) reductase Neighboring gene fructose-1,6-bisphosphatase 2 Neighboring gene glycerol facilitator Neighboring gene cell division factor ZapB

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Catalytic and allosteric properties of glycerol kinase from Escherichia coli. Thorner JW, et al. J Biol Chem, 1973 Jun 10. PMID 4575199
  2. Crystal structures of Escherichia coli glycerol kinase variant S58-->W in complex with nonhydrolyzable ATP analogues reveal a putative active conformation of the enzyme as a result of domain motion. Bystrom CE, et al. Biochemistry, 1999 Mar 23. PMID 10090737
  3. Glycerol kinase from Escherichia coli and an Ala65-->Thr mutant: the crystal structures reveal conformational changes with implications for allosteric regulation. Feese MD, et al. Structure, 1998 Nov 15. PMID 9817843
  4. A single amino acid change in Escherichia coli glycerol kinase abolishes glucose control of glycerol utilization in vivo. Pettigrew DW, et al. J Bacteriol, 1996 May. PMID 8631672, Free PMC Article
  5. Linkage between fructose 1,6-bisphosphate binding and the dimer-tetramer equilibrium of Escherichia coli glycerol kinase: critical behavior arising from change of ligand stoichiometry. Yu P, et al. Biochemistry, 2003 Apr 15. PMID 12680779

See all (68) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. To further elucidate the contributions of the enzymatic (phosphorylation) and alternative (ASTP) functions of GK in adipogenesis, we performed experiments on mammalian GK and E. coli GK. We determined that the ASTP function of GK (which is absent in E. coli GK) plays a greater role than the enzymatic activity in these processes.
    Title: The ATP-stimulated translocation promoter (ASTP) activity of glycerol kinase plays central role in adipogenesis.
  2. point mutation promotes growth by improving glycerol utilization but results in increased carbon wasting as overflow metabolism
    Title: Global metabolic network reorganization by adaptive mutations allows fast growth of Escherichia coli on glycerol.
  3. Functional and metabolic effects of adaptive glycerol kinase (GLPK) mutants in Escherichia coli.
    Title: Functional and metabolic effects of adaptive glycerol kinase (GLPK) mutants in Escherichia coli.
  4. When the functional dimer for glycerol kinase is identified, it is determined that the domain-swap loop is not removed from the catalytic cleft upon dimer formation.
    Title: Oligomeric interactions provide alternatives to direct steric modes of control of sugar kinase/actin/hsp70 superfamily functions by heterotropic allosteric effectors: inhibition of E. coli glycerol kinase.
  5. N-terminus verified by Edman degradation on mature peptide
    Title: Escherichia coli glycerol kinase. Cloning and sequencing of the glpK gene and the primary structure of the enzyme.
  6. GKG230D displays increased enzymatic activity & decreased allosteric regulation by the glycolytic pathway intermediate fructose 1,6-bisphosphate (FBP) compared to wild-type GK.
    Title: Crystal structure of a hyperactive Escherichia coli glycerol kinase mutant Gly230 --> Asp obtained using microfluidic crystallization devices.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables glycerol kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables glycerol kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables glycerol kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables phosphotransferase activity, alcohol group as acceptor IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within DNA damage response IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in carbohydrate metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in glycerol catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in glycerol catabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within glycerol catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in glycerol metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in glycerol metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within glycerol metabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in glycerol-3-phosphate metabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytosol HDA PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
glycerol kinase
NP_418361.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4115714..4117222 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418361.1 glycerol kinase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418361.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9ANG6
    Conserved Domains (1) summary
    PRK00047
    Location:1 → 498
    glpK; glycerol kinase GlpK
Nucleotide Protein
Heading Accession and Version

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