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pfkA 6-phosphofructokinase 1 [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948412, updated on 26-Mar-2025
Official Symbol
pfkA
Official Full Name
6-phosphofructokinase 1
Primary source
ECOCYC:EG10699
Locus tag
b3916
See related
ASAP:ABE-0012789
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3908
Summary
This enzyme is an isozyme with phosphofructokinase-2. [More information is available at EcoCyc: EG10699].
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Sequence:
NC_000913.3 (4107552..4108514)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene Zn(2(+))/Fe(2(+))/Cd(2(+)) exporter Neighboring gene sulfate/thiosulfate ABC transporter periplasmic binding protein Sbp Neighboring gene CDP-diacylglycerol diphosphatase

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. The role of Glu187 in the regulation of phosphofructokinase by phosphoenolpyruvate. Auzat I, et al. J Mol Biol, 1994 Jan 7. PMID 7904653
  2. Persistent binding of MgADP to the E187A mutant of Escherichia coli phosphofructokinase in the absence of allosteric effects. Pham AS, et al. Biochemistry, 2001 Apr 3. PMID 11300795
  3. Reaction path of phosphofructo-1-kinase is altered by mutagenesis and alternative substrates. Wang X, et al. Biochemistry, 2001 Apr 3. PMID 11300773
  4. Crystal structure of unliganded phosphofructokinase from Escherichia coli. Rypniewski WR, et al. J Mol Biol, 1989 Jun 20. PMID 2527305
  5. Urea-induced inactivation, dissociation, and unfolding of the allosteric phosphofructokinase from Escherichia coli. Bras GL, et al. Biochemistry, 1989 Aug 22. PMID 2531001

See all (92) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. All four unique heterotropic interactions between phosphoenolpyruvate and fructose 6-phosphate contributed to the inhibition of pfkA.
    Title: Disentangling the web of allosteric communication in a homotetramer: heterotropic inhibition in phosphofructokinase from Escherichia coli.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 6-phosphofructokinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 6-phosphofructokinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 6-phosphofructokinase activity IEA
Inferred from Electronic Annotation
more info
  
enables 6-phosphofructokinase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables GDP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables fructose-6-phosphate binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables magnesium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables phosphofructokinase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables ribonucleotide binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in canonical glycolysis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in carbohydrate phosphorylation IEA
Inferred from Electronic Annotation
more info
  
involved_in fructose 1,6-bisphosphate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in fructose 6-phosphate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in fructose 6-phosphate metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within glucose catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within glycolytic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in glycolytic process IEA
Inferred from Electronic Annotation
more info
  
involved_in glycolytic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein homotetramerization IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
part_of 6-phosphofructokinase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of 6-phosphofructokinase complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
6-phosphofructokinase 1
NP_418351.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4107552..4108514
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418351.1 6-phosphofructokinase 1 [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418351.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A6C9Q859, A0A7A2XQR7
    Conserved Domains (1) summary
    PRK03202
    Location:2 → 319
    PRK03202; ATP-dependent 6-phosphofructokinase
Nucleotide Protein
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