cpxA sensor histidine kinase CpxA [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: cpxA
Official Full Name: sensor histidine kinase CpxA
Primary source: ECOCYC:EG10163
Locus tag: b3911
See related: ASAP:ABE-0012772
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ecf; ecfB; ECK3904; eup; rssE; ssd
Summary: The sensor protein CpxA is part of the two-component CpxA/CpxR signal transduction system. [More information is available at EcoCyc: EG10163].
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Genomic context

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Sequence:: NC_000913.3 (4103602..4104975, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The CpxRA two-component system of adherent and invasive Escherichia coli contributes to epithelial cell invasion and early-stage intestinal fitness in a dysbiotic mouse model mediated by type 1 fimbriae expression.
Title: The CpxRA two-component system of adherent and invasive Escherichia coli contributes to epithelial cell invasion and early-stage intestinal fitness in a dysbiotic mouse model mediated by type 1 fimbriae expression.
The sensor of the bacterial histidine kinase CpxA is a novel dimer of extracytoplasmic Per-ARNT-Sim domains.
Title: The sensor of the bacterial histidine kinase CpxA is a novel dimer of extracytoplasmic Per-ARNT-Sim domains.
Outer Membrane Channel Protein TolC Regulates Escherichia coli K12 Sensitivity to Plantaricin BM-1 via the CpxR/CpxA Two-Component Regulatory System.
Title: Outer Membrane Channel Protein TolC Regulates Escherichia coli K12 Sensitivity to Plantaricin BM-1 via the CpxR/CpxA Two-Component Regulatory System.
Loss of cell wall integrity genes cpxA and mrcB causes flocculation in Escherichia coli.
Title: Loss of cell wall integrity genes cpxA and mrcB causes flocculation in Escherichia coli.
Structural asymmetry does not indicate hemiphosphorylation in the bacterial histidine kinase CpxA.
Title: Structural asymmetry does not indicate hemiphosphorylation in the bacterial histidine kinase CpxA.
Overall, these data demonstrate that NlpE acts as a sentinel for two important envelope biogenesis processes, namely, lipoprotein sorting and oxidative folding, and they further establish NlpE as a bona fide member of the CpxA-CpxR two-component system.
Title: The Lipoprotein NlpE Is a Cpx Sensor That Serves as a Sentinel for Protein Sorting and Folding Defects in the Escherichia coli Envelope.
Study found that the CpxRA system regulates the virulence properties of Avian pathogenic Escherichia coli. Adherence, invasiveness, motility, production of type 1 fimbriae and biofilm were negatively affected in the DeltacpxA mutant indicating that the CpxA is required for full manifestation of these phenotypes.
Title: The CpxRA stress response system regulates virulence features of avian pathogenic Escherichia coli.
The CpxAR system is widely considered to be responsible for sensing attachment, specifically to hydrophobic surfaces. Using both single-cell and population-level analyses, we confirm RcsCDB activation upon surface contact, but find that the CpxAR system is not activated, in contrast to what had earlier been reported. Thus, the role of CpxAR in surface sensing and initiation of biofilm formation should be reconsidered.
Title: Reassessing the role of the Escherichia coli CpxAR system in sensing surface contact.
The cpxA mutant was attenuated for virulence in the kidney but not in the bladder or urine.
Title: Evaluation of CpxRA as a Therapeutic Target for Uropathogenic Escherichia coli Infections.
X-ray structures of the CpxA reveals allosteric switching mechanism, according to which CpxR binding to one CpxA protomer triggers autophosphorylation in the second protomer.
Title: Structural Coupling between Autokinase and Phosphotransferase Reactions in a Bacterial Histidine Kinase.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables phosphoprotein phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphorelay sensor kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphorelay sensor kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphorelay sensor kinase activity	IEA Inferred from Electronic Annotation more info
enables phosphorelay sensor kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein histidine kinase activity	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables transferase activity, transferring phosphorus-containing groups	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cell adhesion	IEA Inferred from Electronic Annotation more info
involved_in cell adhesion involved in biofilm formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to cell envelope stress	TAS Traceable Author Statement more info	PubMed
involved_in peptidyl-histidine phosphorylation	ISM Inferred from Sequence Model more info	PubMed
involved_in phosphorelay signal transduction system	IEA Inferred from Electronic Annotation more info
involved_in protein autophosphorylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within response to radiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction	IEA Inferred from Electronic Annotation more info
involved_in signal transduction	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info