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polA DNA polymerase I [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948356, updated on 26-Mar-2025

Summary

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Official Symbol
polA
Official Full Name
DNA polymerase I
Primary source
ECOCYC:EG10746
Locus tag
b3863
See related
ASAP:ABE-0012618
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3855; resA
Summary
Residues 324-928 constitute the Klenow fragment. [More information is available at EcoGene: EG10746]. DNA Polymerase I (Pol I) is a multifunctional enzyme that combines a DNA polymerase activity, a 5' to 3' exonuclease activity and a 3' to 5' proofreading exonuclease activity. [More information is available at EcoCyc: EG10746].
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Genomic context

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Sequence:
NC_000913.3 (4046966..4049752)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF945 domain-containing protein YihF Neighboring gene 1-acylglycerol-3-phosphate O-acyltransferase YihG Neighboring gene ncRNA Neighboring gene GTP-binding protein YihA

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Thermodynamic dissection of the polymerizing and editing modes of a DNA polymerase. Bailey MF, et al. J Mol Biol, 2004 Feb 20. PMID 15095980
  2. Discrimination against purine-pyrimidine mispairs in the polymerase active site of DNA polymerase I: a structural explanation. Minnick DT, et al. Proc Natl Acad Sci U S A, 2002 Feb 5. PMID 11830658, Free PMC Article
  3. Participation of active-site carboxylates of Escherichia coli DNA polymerase I (Klenow fragment) in the formation of a prepolymerase ternary complex. Gangurde R, et al. Biochemistry, 2002 Dec 10. PMID 12463755
  4. Functional implications of the Klenow fragment structure. Steitz TA, et al. Biochem Soc Trans, 1986 Apr. PMID 3519313
  5. Interaction of DNA polymerase I (Klenow fragment) with the single-stranded template beyond the site of synthesis. Turner RM Jr, et al. Biochemistry, 2003 Mar 4. PMID 12600204

See all (184) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Single-molecule view of coordination in a multi-functional DNA polymerase.
    Title: Single-molecule view of coordination in a multi-functional DNA polymerase.
  2. E. coli DNA Polymerase I and the Klenow Fragment.
    Title: E. coli DNA Polymerase I and the Klenow Fragment.
  3. Findings indicate an unrealized function of DNA polymerase I (Pol I), and a possible mechanism by which G4 structures can be resolved during DNA replication and/or repair.
    Title: Escherichia coli DNA polymerase I can disrupt G-quadruplex structures during DNA replication.
  4. Authors demonstrate for the first time that the major DNA polymerases (Pol I and Pol III) and DNA ligase are directly involved with oligo recombination.
    Title: Bacterial DNA polymerases participate in oligonucleotide recombination.
  5. Pol I catalyzes DNA synthesis past N6-dA-linked peptide cross-links and is likely to play an role in cellular bypass of large major groove DNA lesions.
    Title: Role of high-fidelity Escherichia coli DNA polymerase I in replication bypass of a deoxyadenosine DNA-peptide cross-link.
  6. analysis of complexes of DNA polymerase I (Klenow fragment) for base and sugar discrimination during nucleotide substrate selection
    Title: Distinct complexes of DNA polymerase I (Klenow fragment) for base and sugar discrimination during nucleotide substrate selection.
  7. Data show that dnaX36 strains have an increased requirement for DNA polymerase I.
    Title: Role of accessory DNA polymerases in DNA replication in Escherichia coli: analysis of the dnaX36 mutator mutant.
  8. Findings indicate that elevated levels of Pol I partially suppress the temperature-sensitive growth phenotype of the dnaN159 mutant strain.
    Title: Role of Escherichia coli DNA polymerase I in conferring viability upon the dnaN159 mutant strain.
  9. mechanism in which Arg668 forms a hydrogen bonding fork between the minor groove of the primer terminus and the ring oxygen of the deoxyribose moiety of the incoming dNTP to align the 3'-hydroxyl group with the alpha-phosphate of the dNTP
    Title: Fidelity of mispair formation and mispair extension is dependent on the interaction between the minor groove of the primer terminus and Arg668 of DNA polymerase I of Escherichia coli.
  10. mutant(Delta)polA is defective in base mismatch correction
    Title: Escherichia coli mutator (Delta)polA is defective in base mismatch correction: the nature of in vivo DNA replication errors.
Submit: New GeneRIF Correction See all GeneRIFs (13)

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 3'-5' exonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 3'-5' exonuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables 5'-3' exonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 5'-3' exonuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA polymerase activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA-directed DNA polymerase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA-directed DNA polymerase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA-directed DNA polymerase activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA-directed DNA polymerase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity, acting on a nucleic acid IEA
Inferred from Electronic Annotation
more info
  
enables exonuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables nuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables nucleic acid binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotidyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in DNA biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA damage response IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within DNA repair IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within DNA replication IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in DNA replication IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within DNA-templated DNA replication IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in DNA-templated DNA replication IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within base-excision repair IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in double-strand break repair IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in nucleobase-containing compound metabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
DNA polymerase I
NP_418300.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4046966..4049752
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418300.1 DNA polymerase I [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418300.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A895NWI0
    Conserved Domains (1) summary
    PRK05755
    Location:6 → 928
    PRK05755; DNA polymerase I; Provisional
Nucleotide Protein
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