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tatA twin arginine protein translocation system - TatA protein [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948321, updated on 20-Feb-2025

Summary

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Official Symbol
tatA
Official Full Name
twin arginine protein translocation system - TatA protein
Primary source
ECOCYC:G7806
Locus tag
b3836
See related
ASAP:ABE-0012541
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3830; mttA1; yigT
Summary
[More information is available at EcoGene: EG11478]. TatA is a subunit of the TatABCE (twin-arginine translocation) complex for the export of folded proteins across the cytoplasmic membrane. [More information is available at EcoCyc: G7806].
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Genomic context

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Sequence:
NC_000913.3 (4021945..4022214)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene bifunctional 2-octaprenyl-6-methoxy-1,4-benzoquinol methylase and demethylmenaquinone methyltransferase Neighboring gene ubiquinone biosynthesis accessory factor UbiJ Neighboring gene ncRNA Neighboring gene ubiquinone biosynthesis protein UbiB Neighboring gene twin arginine protein translocation system - TatB protein Neighboring gene twin arginine protein translocation system - TatC protein

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Live cell imaging shows reversible assembly of the TatA component of the twin-arginine protein transport system. Alcock F, et al. Proc Natl Acad Sci U S A, 2013 Sep 17. PMID 24003141, Free PMC Article
  2. Contributions of the transmembrane domain and a key acidic motif to assembly and function of the TatA complex. Warren G, et al. J Mol Biol, 2009 Apr 24. PMID 19268473
  3. Dual topology of the Escherichia coli TatA protein. Gouffi K, et al. J Biol Chem, 2004 Mar 19. PMID 14701831
  4. Variable stoichiometry of the TatA component of the twin-arginine protein transport system observed by in vivo single-molecule imaging. Leake MC, et al. Proc Natl Acad Sci U S A, 2008 Oct 7. PMID 18832162, Free PMC Article
  5. Identification of key regions within the Escherichia coli TatAB subunits. Barrett CM, et al. FEBS Lett, 2003 Feb 27. PMID 12606028

See all (127) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The polar amino acid in the TatA transmembrane helix is not strictly necessary for protein function.
    Title: The polar amino acid in the TatA transmembrane helix is not strictly necessary for protein function.
  2. TatA and TatB generate a hydrophobic mismatch important for the function and assembly of the Tat translocon in Escherichia coli.
    Title: TatA and TatB generate a hydrophobic mismatch important for the function and assembly of the Tat translocon in Escherichia coli.
  3. Hydrophobic mismatch is a key factor in protein transport across lipid bilayer membranes via the Tat pathway.
    Title: Hydrophobic mismatch is a key factor in protein transport across lipid bilayer membranes via the Tat pathway.
  4. Ferric Citrate Regulator FecR Is Translocated across the Bacterial Inner Membrane via a Unique Twin-Arginine Transport-Dependent Mechanism.
    Title: Ferric Citrate Regulator FecR Is Translocated across the Bacterial Inner Membrane via a Unique Twin-Arginine Transport-Dependent Mechanism.
  5. revealed that substrate binding causes the TatA hinge region and the N-terminal part of the TatA amphipathic helix to move toward the membrane surface
    Title: The TatA component of the twin-arginine translocation system locally weakens the cytoplasmic membrane of Escherichia coli upon protein substrate binding.
  6. The authors observe that substitutions of charged residues located in the TatA amphipathic helix lock TatA in an assembled state, suggesting that these charged residues play a critical role in the protein translocation step that follows TatA assembly.
    Title: In vivo experiments do not support the charge zipper model for Tat translocase assembly.
  7. It has been concluded that Tat signal peptides play roles in substrate targeting and in triggering assembly of the active TatABC translocase.
    Title: A signal sequence suppressor mutant that stabilizes an assembled state of the twin arginine translocase.
  8. In the TatBC receptor complex the transmembrane helix of each TatB molecule is sandwiched between two TatC molecules, with one of the inter-subunit interfaces incorporating a functionally important cluster of interacting polar residues. Unexpectedly, the authors find that TatA also associates with TatC at the polar cluster site.
    Title: Assembling the Tat protein translocase.
  9. Twin-arginine translocation (Tat) system TatA exhibits a uniform distribution throughout the inner membrane and that altering the expression of TatBC shows a uncharacterised distribution of TatA in the inner membrane.
    Title: TatA complexes exhibit a marked change in organisation in response to expression of the TatBC complex.
  10. Distributed TatA thus may serve to accompany membrane-interacting Tat substrates to the few TatBC spots in the cells.
    Title: TatBC-independent TatA/Tat substrate interactions contribute to transport efficiency.
Submit: New GeneRIF Correction See all GeneRIFs (25)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables lipid binding EXP
Inferred from Experiment
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein transmembrane transporter activity IEA
Inferred from Electronic Annotation
more info
  
enables protein transmembrane transporter activity IGI
Inferred from Genetic Interaction
more info
 PubMed 
enables proton motive force dependent protein transmembrane transporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within intracellular protein transmembrane transport EXP
Inferred from Experiment
more info
 PubMed 
acts_upstream_of_or_within intracellular protein transmembrane transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein transport IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within protein transport by the Tat complex EXP
Inferred from Experiment
more info
 PubMed 
acts_upstream_of_or_within protein transport by the Tat complex IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein transport by the Tat complex IEA
Inferred from Electronic Annotation
more info
  
involved_in protein transport by the Tat complex NAS
Non-traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
part_of TAT protein transport complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of TAT protein transport complex IEA
Inferred from Electronic Annotation
more info
  
part_of TAT protein transport complex NAS
Non-traceable Author Statement
more info
 PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane ISO
Inferred from Sequence Orthology
more info
 PubMed 
located_in plasma membrane NAS
Non-traceable Author Statement
more info
 PubMed 

General protein information

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Preferred Names
twin arginine protein translocation system - TatA protein

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4021945..4022214
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418280.4 twin arginine protein translocation system - TatA protein [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418280.4

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0AAJ3NV36
    Conserved Domains (1) summary
    PRK03554
    Location:1 → 89
    tatA; Sec-independent protein translocase subunit TatA
Nucleotide Protein
Heading Accession and Version

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