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pldA outer membrane phospholipase A [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948307, updated on 4-Feb-2026
Official Symbol
pldA
Official Full Name
outer membrane phospholipase A
Primary source
ECOCYC:EG10738
Locus tag
b3821
See related
ASAP:ABE-0012479
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3815
Summary
PldA is the Phospholipase A1 precursor which is a member of EC 3.1.1.32. [More information is available at EcoCyc: EG10738].
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Sequence:
NC_000913.3 (4004862..4005731)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative transporter RarD Neighboring gene acyl-CoA thioesterase YigI Neighboring gene ATP-dependent DNA helicase RecQ Neighboring gene L-threonine exporter

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Dimerization regulates the enzymatic activity of Escherichia coli outer membrane phospholipase A. Dekker N, et al. J Biol Chem, 1997 Feb 7. PMID 9013551
  2. Outer-membrane phospholipase A: known structure, unknown biological function. Dekker N. Mol Microbiol, 2000 Feb. PMID 10692149
  3. Structural evidence for dimerization-regulated activation of an integral membrane phospholipase. Snijder HJ, et al. Nature, 1999 Oct 14. PMID 10537112
  4. Outer membrane phospholipase A is dimeric in phospholipid bilayers: a cross-linking and fluorescence resonance energy transfer study. Ubarretxena-Belandia I, et al. Biochemistry, 1999 Jun 1. PMID 10353852
  5. Bacteriocin release protein triggers dimerization of outer membrane phospholipase A in vivo. Dekker N, et al. J Bacteriol, 1999 May. PMID 10322034, Free PMC Article

See all (49) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Upon the addition of Ca(2+), OmpLA was activated and degraded the membrane with a turnover of ~2 phospholipid molecules per second and per OmpLA dimer until most of the membrane phospholipids were hydrolyzed and the protein became tightly packed.
    Title: Real-time Visualization of Phospholipid Degradation by Outer Membrane Phospholipase A using High-Speed Atomic Force Microscopy.
  2. The crystal structure of the dimeric state of the transmembrane beta-barrel protein outer membrane phospholipase A (OMPLA) revealed an intermolecular hydrogen bond mediated by a highly conserved glutamine side-chain (Q94).
    Title: The role of a hydrogen bonding network in the transmembrane beta-barrel OMPLA.
  3. N-terminus verified by Edman degradation on mature peptide
    Title: The DNA sequence encoding pldA gene, the structural gene for detergent-resistant phospholipase A of E. coli.
  4. These energetic measurements not only provide insight into the activation of OMPLA, but they also represent the first quantitative investigation of the association energetics of a transmembrane beta-barrel.
    Title: Energetics of outer membrane phospholipase A (OMPLA) dimerization.
Submit: New GeneRIF Correction

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables A2-type glycerophospholipase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables A2-type glycerophospholipase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables A2-type glycerophospholipase activity IEA
Inferred from Electronic Annotation
more info
  
enables A2-type glycerophospholipase activity TAS
Traceable Author Statement
more info
 PubMed 
enables calcium ion binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables calcium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables glycerophospholipase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables glycerophospholipase activity IEA
Inferred from Electronic Annotation
more info
  
enables glycerophospholipid phospholipase A1 activity IEA
Inferred from Electronic Annotation
more info
  
enables glycerophospholipid phospholipase A1 activity TAS
Traceable Author Statement
more info
 PubMed 
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables phosphatidylcholine lysophospholipase A1 activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in lipid catabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in lipid metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within phosphatidylglycerol metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cell outer membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cell outer membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cell outer membrane IEA
Inferred from Electronic Annotation
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
outer membrane phospholipase A
NP_418265.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    4004862..4005731
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418265.1 outer membrane phospholipase A

    See identical proteins and their annotated locations for NP_418265.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    C3SK99
    Conserved Domains (1) summary
    PRK10763
    Location:1 → 289
    PRK10763; phospholipase A; Provisional
Nucleotide Protein
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