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rep ATP-dependent DNA helicase Rep [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948292, updated on 10-Feb-2026
Official Symbol
rep
Official Full Name
ATP-dependent DNA helicase Rep
Primary source
ECOCYC:EG10837
Locus tag
b3778
See related
ASAP:ABE-0012344
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
dasC; ECK3770; mbrA; mmrA
Summary
Rep is an ATP-dependent helicase that is required for genomic replication in Escherichia coli and some bacteriophages . [More information is available at EcoCyc: EG10837].
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Sequence:
NC_000913.3 (3960677..3962698)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene peptidyl-prolyl cis-trans isomerase C Neighboring gene pseudo Neighboring gene guanosine-5'-triphosphate,3'-diphosphate phosphatase Neighboring gene ATP-dependent RNA helicase RhlB

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Complexes of Rep protein with ATP and DNA as a basis for helicase action. Arai N, et al. J Biol Chem, 1981 May 25. PMID 6112227
  2. Initiation and re-initiation of DNA unwinding by the Escherichia coli Rep helicase. Ha T, et al. Nature, 2002 Oct 10. PMID 12374984
  3. E. coli Rep oligomers are required to initiate DNA unwinding in vitro. Cheng W, et al. J Mol Biol, 2001 Jul 6. PMID 11428893
  4. A two-site kinetic mechanism for ATP binding and hydrolysis by E. coli Rep helicase dimer bound to a single-stranded oligodeoxynucleotide. Hsieh J, et al. J Mol Biol, 1999 Apr 30. PMID 10329141
  5. Kinetic mechanism for the sequential binding of two single-stranded oligodeoxynucleotides to the Escherichia coli Rep helicase dimer. Bjornson KP, et al. Biochemistry, 1998 Jan 20. PMID 9454579

See all (82) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Single-molecule visualization of stalled replication-fork rescue by the Escherichia coli Rep helicase.
    Title: Single-molecule visualization of stalled replication-fork rescue by the Escherichia coli Rep helicase.
  2. Regulation of E. coli Rep helicase activity by PriC.
    Title: Regulation of E. coli Rep helicase activity by PriC.
  3. E. coli Rep helicase and RecA recombinase unwind G4 DNA and are important for resistance to G4-stabilizing ligands.
    Title: E. coli Rep helicase and RecA recombinase unwind G4 DNA and are important for resistance to G4-stabilizing ligands.
  4. Findings suggest two Rep-replisome populations in vivo: one continually associating with DnaB then translocating away to aid nucleoprotein barrier removal ahead of the fork, another assisting PriC-dependent reloading of DnaB if replisome progression fails.
    Title: Single-molecule live cell imaging of Rep reveals the dynamic interplay between an accessory replicative helicase and the replisome.
  5. Rep and UvrD helicases operate on DNA at the replication fork whereas DinG helicase acts via a different mechanism
    Title: Direct removal of RNA polymerase barriers to replication by accessory replicative helicases.
  6. We provide a quantitative model of the factors that enhance unwinding processivity. Our work sheds light on the mechanisms of regulation of unwinding by Rep-like helicases.
    Title: Regulation of Rep helicase unwinding by an auto-inhibitory subdomain.
  7. Data show that single-stranded DNA binding proteins (SSB)-interacting proteins RecO, RecG, PriA, and accessory helicase Rep do not correlate with replication at relication forks.
    Title: Are the SSB-Interacting Proteins RecO, RecG, PriA and the DnaB-Interacting Protein Rep Bound to Progressing Replication Forks in Escherichia coli?
  8. The authors demonstrate that Pol IV interacts in vitro with Rep DNA helicase and that this interaction enhances Rep's helicase activity.
    Title: Escherichia coli Rep DNA helicase and error-prone DNA polymerase IV interact physically and functionally.
  9. After replication blockage by an inverted rrn, Rep in conjunction with DinG or UvrD removes RNA polymerase, a task that is fulfilled in its absence by the SOS-induced DinG and UvrD helicases.
    Title: The helicases DinG, Rep and UvrD cooperate to promote replication across transcription units in vivo.
  10. N-terminus verified by Edman degradation on mature peptide
    Title: Escherichia coli rep gene: identification of the promoter and N terminus of the rep protein.
Submit: New GeneRIF Correction See all GeneRIFs (15)
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 3'-5' DNA helicase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 3'-5' DNA helicase activity IEA
Inferred from Electronic Annotation
more info
  
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA helicase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA helicase activity IEA
Inferred from Electronic Annotation
more info
  
enables helicase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables isomerase activity IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables single-stranded DNA binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in DNA metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA replication IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA replication NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in DNA replication, synthesis of primer NAS
Non-traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within bacterial-type DNA replication IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in recombinational repair IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in replication fork processing NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in response to radiation IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within response to radiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of cytoplasmic replisome EXP
Inferred from Experiment
more info
 PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of protein-containing complex IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
ATP-dependent DNA helicase Rep
YP_026251.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3960677..3962698
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. YP_026251.1 ATP-dependent DNA helicase Rep

    See identical proteins and their annotated locations for YP_026251.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A2X1NUI7
    Conserved Domains (1) summary
    PRK10919
    Location:1 → 672
    PRK10919; ATP-dependent DNA helicase Rep; Provisional
Nucleotide Protein
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