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ilvA threonine deaminase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948287, updated on 19-Sep-2024

Summary

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Official Symbol
ilvA
Official Full Name
threonine deaminase
Primary source
ECOCYC:EG10493
Locus tag
b3772
See related
ASAP:ABE-0012321
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3764; ile
Summary
N-terminal sequence of cloned IlvA shows fMet is clipped, but natural RBS was not used (Eisenstein, 1991) so it is not Verified, but the ATG start is reliable based on comparisons to Salmonella. [More information is available at EcoGene: EG10493]. Threonine deaminase (IlvA) carries out the first step in the synthesis of isoleucine. [More information is available at EcoCyc: EG10493].
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Genomic context

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Sequence:
NC_000913.3 (3955331..3956875)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene branched-chain-amino-acid aminotransferase Neighboring gene dihydroxy-acid dehydratase Neighboring gene DNA-binding transcriptional dual regulator IlvY Neighboring gene ketol-acid reductoisomerase (NADP(+))

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Study and reengineering of the binding sites and allosteric regulation of biosynthetic threonine deaminase by isoleucine and valine in Escherichia coli. Chen L, et al. Appl Microbiol Biotechnol, 2013 Apr. PMID 22669632
  2. Homocysteine toxicity in Escherichia coli is caused by a perturbation of branched-chain amino acid biosynthesis. Tuite NL, et al. J Bacteriol, 2005 Jul. PMID 15968045, Free PMC Article
  3. REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. 3. MAP ORDER OF THE STRUCTURAL GENES AND OPERATOR GENES. RAMAKRISHNAN T, et al. J Bacteriol, 1965 Mar. PMID 14273641, Free PMC Article
  4. REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. II. IDENTIFICATION OF TWO OPERATOR GENES. RAMAKRISHNAN T, et al. J Bacteriol, 1965 Mar. PMID 14273640, Free PMC Article
  5. Evidence for a negative-feedback mechanism in the biosynthesis of isoleucine. UMBARGER HE. Science, 1956 May 11. PMID 13324101

See all (51) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The residues R362, E442, G445, A446, Y369, I460, and S461 only interact with isoleucine while E347, G350, and F352 are involved not only in the isoleucine binding but also in the valine binding.
    Title: Study and reengineering of the binding sites and allosteric regulation of biosynthetic threonine deaminase by isoleucine and valine in Escherichia coli.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables L-serine ammonia-lyase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables amino acid binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables pyridoxal phosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables pyridoxal phosphate binding IEA
Inferred from Electronic Annotation
more info
  
enables threonine deaminase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables threonine deaminase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables threonine deaminase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in L-serine catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within branched-chain amino acid biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in isoleucine biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within isoleucine biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in isoleucine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within isoleucine biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in threonine catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in threonine metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
threonine deaminase
NP_418220.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3955331..3956875
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418220.1 threonine deaminase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418220.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A0B1BMI0, A0A140ND38, A0A8T6A3J9
    Conserved Domains (1) summary
    PRK09224
    Location:13 → 513
    PRK09224; threonine ammonia-lyase IlvA
Nucleotide Protein
Heading Accession and Version

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