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ilvD dihydroxy-acid dehydratase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948277, updated on 3-Jun-2026
Official Symbol
ilvD
Official Full Name
dihydroxy-acid dehydratase
Primary source
ECOCYC:EG10496
Locus tag
b3771
See related
ASAP:ABE-0012318
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3763
Summary
Dihydroxy-acid dehydratase carries out the penultimate step in both the isoleucine and valine biosynthesis pathways. [More information is available at EcoCyc: EG10496].
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Sequence:
NC_000913.3 (3953478..3955328)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene pseudo Neighboring gene acetolactate synthase II subunit IlvM Neighboring gene branched-chain-amino-acid aminotransferase Neighboring gene threonine deaminase Neighboring gene DNA-binding transcriptional dual regulator IlvY

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Reactivity of nitric oxide with the [4Fe-4S] cluster of dihydroxyacid dehydratase from Escherichia coli. Duan X, et al. Biochem J, 2009 Feb 1. PMID 18945212, Free PMC Article
  2. Physical organization of the ilvEDAC genes of Escherichia coli strain K-12. McCorkle GM, et al. Proc Natl Acad Sci U S A, 1978 Jan. PMID 343113, Free PMC Article
  3. Dihydroxyacid dehydratase: the site of hyperbaric oxygen poisoning in branch-chain amino acid biosynthesis. Brown OR, et al. Biochem Biophys Res Commun, 1978 Dec 14. PMID 367373
  4. An activity stain for dihydroxy-acid dehydratase. Kuo CF, et al. Anal Biochem, 1987 Aug 1. PMID 2445227
  5. A reverse-phase high-performance liquid chromatography assay for dihydroxy-acid dehydratase. Smyk-Randall EM, et al. Anal Biochem, 1987 Aug 1. PMID 3314584

See all (36) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. N-terminus verified by Edman degradation on mature peptide
    Title: The role and properties of the iron-sulfur cluster in Escherichia coli dihydroxy-acid dehydratase.
  2. This approach identified the comprehensive E. coli NO response network and evinced that nitric oxide (NO) halts bacterial growth via inhibition of the branched-chain amino acid biosynthesis enzyme dihydroxyacid dehydratase.
    Title: Integrated network analysis identifies nitric oxide response networks and dihydroxyacid dehydratase as a crucial target in Escherichia coli.
Submit: New GeneRIF Correction
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 2 iron, 2 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables 4 iron, 4 sulfur cluster binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables dihydroxy-acid dehydratase activity EXP
Inferred from Experiment
more info
 PubMed 
enables dihydroxy-acid dehydratase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables dihydroxy-acid dehydratase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydro-lyase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables hydro-lyase activity IEA
Inferred from Electronic Annotation
more info
  
enables iron-sulfur cluster binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables magnesium ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within L-valine biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in L-valine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in branched-chain amino acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
dihydroxy-acid dehydratase
YP_026248.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3953478..3955328
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. YP_026248.1 dihydroxy-acid dehydratase

    See identical proteins and their annotated locations for YP_026248.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A376FUL6
    Conserved Domains (1) summary
    PRK12448
    Location:2 → 616
    PRK12448; dihydroxy-acid dehydratase; Provisional
Nucleotide Protein
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