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dnaN beta sliding clamp [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948218, updated on 26-Mar-2025
Official Symbol
dnaN
Official Full Name
beta sliding clamp
Primary source
ECOCYC:EG10242
Locus tag
b3701
See related
ASAP:ABE-0012098
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3693
Summary
The beta subunit of DNA polymerase III dimerizes to form the sliding clamp that links the core polymerase to DNA and allows processive DNA replication. [More information is available at EcoCyc: EG10242].
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Sequence:
NC_000913.3 (3881221..3882321, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA gyrase subunit B Neighboring gene recombination mediator protein RecF Neighboring gene chromosomal replication initiator protein DnaA Neighboring gene protein YsdD

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Structure of a small-molecule inhibitor of a DNA polymerase sliding clamp. Georgescu RE, et al. Proc Natl Acad Sci U S A, 2008 Aug 12. PMID 18678908, Free PMC Article
  2. Clamping down on transposon targeting. Chandler M. Cell, 2009 Aug 21. PMID 19703389
  3. The dnaN gene codes for the beta subunit of DNA polymerase III holoenzyme of escherichia coli. Burgers PM, et al. Proc Natl Acad Sci U S A, 1981 Sep. PMID 6458041, Free PMC Article
  4. ATP activation of DNA polymerase III holoenzyme from Escherichia coli. II. Initiation complex: stoichiometry and reactivity. Burgers PM, et al. J Biol Chem, 1982 Oct 10. PMID 6749841
  5. Purification and characterization of the beta subunit of the DNA polymerase III holoenzyme of Escherichia coli. Johanson KO, et al. J Biol Chem, 1980 Nov 25. PMID 6776124

See all (162) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Elevated Levels of the Escherichia coli nrdAB-Encoded Ribonucleotide Reductase Counteract the Toxicity Caused by an Increased Abundance of the beta Clamp.
    Title: Elevated Levels of the Escherichia coli nrdAB-Encoded Ribonucleotide Reductase Counteract the Toxicity Caused by an Increased Abundance of the β Clamp.
  2. The Mutant beta(E202K) Sliding Clamp Protein Impairs DNA Polymerase III Replication Activity.
    Title: The Mutant β(E202K) Sliding Clamp Protein Impairs DNA Polymerase III Replication Activity.
  3. In Bacillus subtilis, the interaction stimulates the endonuclease activity of MutL and it is critical for DNA mismatch repair. Conversely, disrupting the interaction between Escherichia coli MutL and the b-clamp only causes a mild mutator phenotype. Here, we determined the structures of the regulatory domains of E. coli and B. subtilis MutL bound to their respective b-clamps
    Title: Binding of the regulatory domain of MutL to the sliding β-clamp is species specific.
  4. Results demonstrate that the beta-clamp of the Escherichia coli replisome strongly inhibits nick translation on the DNA substrate. beta-clamp inhibits the strand displacement activity of Pol I by interfering with the interaction between the finger subdomain of Pol I and the downstream primer-template junction. beta-clamp stimulates the 5' exonuclease property of Pol I to cleave single nucleotides or shorter oligonucleo...
    Title: Escherichia coli β-clamp slows down DNA polymerase I dependent nick translation while accelerating ligation.
  5. structural and mutational analyses of the Hda-beta clamp complex indicate that the interaction of the beta clamp with Hda controls the ability of Hda to interact with DnaA.
    Title: Dynamic assembly of Hda and the sliding clamp in the regulation of replication licensing.
  6. The Escherichia coli beta clamp can simultaneously bind the replicative polymerase (Pol) III and the conserved Y-family Pol IV, enabling exchange of the two polymerases and rapid bypass of a Pol IV cognate lesion.
    Title: Polymerase exchange on single DNA molecules reveals processivity clamp control of translesion synthesis.
  7. A structural model of the alphaepsilontheta;:beta2 replicase complex reveals a compact, but still flexible, structure of the complex.
    Title: Proofreading exonuclease on a tether: the complex between the E. coli DNA polymerase III subunits α, epsilon, θ and β reveals a highly flexible arrangement of the proofreading domain.
  8. DNA polymerase III beta may stimulate the endonuclease activity of MutL through its direct interaction with the C-terminal domain of MutL.
    Title: The endonuclease domain of MutL interacts with the β sliding clamp.
  9. Escherichia coli cells with a point mutation in the dnaN gene causing the amino acid change Gly157 to Cys, were found to underinitiate replication and grow with a reduced origin and DNA concentration.
    Title: The G157C mutation in the Escherichia coli sliding clamp specifically affects initiation of replication.
  10. Study shows that the little finger domain of PolIV-beta interaction stabilizes the clamp-polymerase complex in vitro and is necessary for the access of PolIV to ongoing replication forks in vivo.
    Title: Distinct beta-clamp interactions govern the activities of the Y family PolIV DNA polymerase.
Submit: New GeneRIF Correction See all GeneRIFs (17)
Products Interactant Other Gene Complex Source Pubs Description

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 3'-5' exonuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA-directed DNA polymerase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables nucleotidyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within DNA damage response IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in DNA replication IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA strand elongation involved in DNA replication IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within DNA strand elongation involved in DNA replication IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in DNA-templated DNA replication NAS
Non-traceable Author Statement
more info
 PubMed 
acts_upstream_of_or_within bacterial-type DNA replication IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within error-prone translesion synthesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of DNA-templated DNA replication initiation NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in regulation of DNA-templated DNA replication initiation NAS
Non-traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
part_of DNA polymerase III complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of DNA polymerase III complex IEA
Inferred from Electronic Annotation
more info
  
part_of DNA polymerase III complex NAS
Non-traceable Author Statement
more info
 PubMed 
part_of Hda-beta clamp complex IPI
Inferred from Physical Interaction
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol HDA PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of replication inhibiting complex IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of replisome NAS
Non-traceable Author Statement
more info
 PubMed 
Preferred Names
beta sliding clamp

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3881221..3882321 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418156.1 beta sliding clamp [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418156.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A6C9Q8B9
    Conserved Domains (1) summary
    PRK05643
    Location:1 → 366
    PRK05643; DNA polymerase III subunit beta; Validated
Nucleotide Protein
Heading Accession and Version

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