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recG ATP-dependent DNA helicase RecG [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948162, updated on 25-Jun-2025
Official Symbol
recG
Official Full Name
ATP-dependent DNA helicase RecG
Primary source
ECOCYC:EG10829
Locus tag
b3652
See related
ASAP:ABE-0011939
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3642; radC; spoV
Summary
See the ruvABC entries for additional information. otherPDB (T. maritima): [More information is available at EcoGene: EG10829]. Regulation has been described . [More information is available at EcoCyc: EG10829].
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Sequence:
NC_000913.3 (3825210..3827291)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene bifunctional (p)ppGpp synthase/hydrolase SpoT Neighboring gene tRNA (Gm18) 2'-O-methyltransferase Neighboring gene glutamate:sodium symporter Neighboring gene xanthine:H(+) symporter XanP

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Genetic analysis of the recG locus of Escherichia coli K-12 and of its role in recombination and DNA repair. Lloyd RG, et al. J Bacteriol, 1991 Feb. PMID 1846849, Free PMC Article
  2. Ruv and recG genes and the induced precise excision of Tn10 in Escherichia coli. Nagel R, et al. Mutat Res, 1994 Nov 1. PMID 7526163
  3. Genetic recombination in Escherichia coli. IV. Isolation and characterization of recombination-deficiency mutants of Escherichia coli K12. Storm PK, et al. Mutat Res, 1971 Sep. PMID 4938964
  4. Processing of recombination intermediates by the RecG and RuvAB proteins of Escherichia coli. Lloyd RG, et al. Nucleic Acids Res, 1993 Apr 25. PMID 8388095, Free PMC Article
  5. Characterization of the ATPase activity of the Escherichia coli RecG protein reveals that the preferred cofactor is negatively supercoiled DNA. Slocum SL, et al. J Mol Biol, 2007 Mar 30. PMID 17292398, Free PMC Article

See all (91) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Interaction with the carboxy-terminal tip of SSB is critical for RecG function in E. coli.
    Title: Interaction with the carboxy-terminal tip of SSB is critical for RecG function in E. coli.
  2. DNA Helicase-SSB Interactions Critical to the Regression and Restart of Stalled DNA Replication forks in Escherichia coli.
    Title: DNA Helicase-SSB Interactions Critical to the Regression and Restart of Stalled DNA Replication forks in Escherichia coli.
  3. Resolving Toxic DNA repair intermediates in every E. coli replication cycle: critical roles for RecG, Uup and RadD.
    Title: Resolving Toxic DNA repair intermediates in every E. coli replication cycle: critical roles for RecG, Uup and RadD.
  4. Data show that cells lacking ATP-dependent DNA helicase RecG (RecG) have indicated substantial over-replication of sequences in the termination region.
    Title: Chromosomal over-replication in Escherichia coli recG cells is triggered by replication fork fusion and amplified if replichore symmetry is disturbed.
  5. results provide mechanistic insights into the modes of interaction of RecG with the replication fork, suggesting a novel role of RecG in the repair of stalled DNA replication forks.
    Title: Dynamics of the Interaction of RecG Protein with Stalled Replication Forks.
  6. Data suggest that RecG and RuvABC catalyze alternative steps in DNA repair/recombination and collaborate to stabilize DNA during repair of double-strand DNA breaks; RecG may prevent DNA amplification at D-loops formed by recombination and at arrested replication forks. (RecG = RecG DNA helicase; Ruv = Holliday junction DNA helicase subunit) [REVIEW]
    Title: RecG controls DNA amplification at double-strand breaks and arrested replication forks.
  7. interaction occurs via the wedge domain of RecG and the intrinsically disordered linker (IDL) of SSB, in a manner similar to that of SH3 domains binding to PXXP motif-containing ligands in eukaryotic cells.
    Title: SSB and the RecG DNA helicase: an intimate association to rescue a stalled replication fork.
  8. Data show that single-stranded DNA binding proteins (SSB)-interacting proteins RecO, RecG, PriA, and accessory helicase Rep do not correlate with replication at relication forks.
    Title: Are the SSB-Interacting Proteins RecO, RecG, PriA and the DnaB-Interacting Protein Rep Bound to Progressing Replication Forks in Escherichia coli?
  9. Once RecG has regressed the fork, it will dissociate leaving the now relaxed, Holliday junction-like DNA, available for further processing by enzymes such as RuvAB. [review]
    Title: I came to a fork in the DNA and there was RecG.
  10. Regression of replication forks stalled by leading-strand template damage: both RecG and RuvAB catalyze regression, but RuvC cleaves the holliday junctions formed by RecG preferentially.
    Title: Regression of replication forks stalled by leading-strand template damage: I. Both RecG and RuvAB catalyze regression, but RuvC cleaves the holliday junctions formed by RecG preferentially.
Submit: New GeneRIF Correction See all GeneRIFs (21)

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables DNA helicase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA helicase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables double-stranded DNA helicase activity IEA
Inferred from Electronic Annotation
more info
  
enables forked DNA-dependent helicase activity IEA
Inferred from Electronic Annotation
more info
  
enables four-way junction helicase activity IEA
Inferred from Electronic Annotation
more info
  
enables isomerase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables single-stranded 3'-5' DNA helicase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in DNA repair IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in replication fork reversal IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within response to radiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of Holliday junction helicase complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
Preferred Names
ATP-dependent DNA helicase RecG

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3825210..3827291
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418109.1 ATP-dependent DNA helicase RecG [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418109.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A7I8YJK3
    Conserved Domains (1) summary
    PRK10917
    Location:1 → 693
    PRK10917; ATP-dependent DNA helicase RecG; Provisional
Nucleotide Protein
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