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waaJ UDP-glucose:(glucosyl)LPS alpha-1,2-glucosyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948142, updated on 19-Nov-2025
Official Symbol
waaJ
Official Full Name
UDP-glucose:(glucosyl)LPS alpha-1,2-glucosyltransferase
Primary source
ECOCYC:EG11353
Locus tag
b3626
See related
ASAP:ABE-0011858
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3616; rfaJ; waaR
Summary
The lipopolysaccharide of Escherichia coli K-12 consists of two major components: the hydrophobic lipid A moiety inserted into the outer membrane and the phosphorylated core oligosaccharide . [More information is available at EcoCyc: EG11353].
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Sequence:
NC_000913.3 (3800983..3801999, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene lipopolysaccharide core biosynthesis protein WaaZ Neighboring gene lipopolysaccharide core heptose (II) kinase Neighboring gene UDP-D-glucose:(glucosyl)LPS alpha-1,3-glucosyltransferase Neighboring gene UDP-D-galactose:(glucosyl)lipopolysaccharide-1,6-D-galactosyltransferase

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Recombinant Escherichia coli K5 strain with the deletion of waaR gene decreases the molecular weight of the heparosan capsular polysaccharide. Huang H, et al. Appl Microbiol Biotechnol, 2016 Sep. PMID 27079575
  2. The C-terminal Domain of the Escherichia coli WaaJ glycosyltransferase is important for catalytic activity and membrane association. Leipold MD, et al. J Biol Chem, 2007 Jan 12. PMID 17090541
  3. Mutations in the waaR gene of Escherichia coli which disrupt lipopolysaccharide outer core biosynthesis affect cell surface retention of group 2 capsular polysaccharides. Taylor CM, et al. J Bacteriol, 2006 Feb. PMID 16428421, Free PMC Article
  4. Four critical aspartic acid residues potentially involved in the catalytic mechanism of Escherichia coli K-12 WaaR. Shibayama K, et al. FEMS Microbiol Lett, 1999 May 1. PMID 10234827
  5. Genetic analysis of lipopolysaccharide core biosynthesis by Escherichia coli K-12: insertion mutagenesis of the rfa locus. Austin EA, et al. J Bacteriol, 1990 Sep. PMID 2168379, Free PMC Article

See all (29) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The waaR deletion mutant showed improvement of capsule polysaccharide yield.
    Title: Recombinant Escherichia coli K5 strain with the deletion of waaR gene decreases the molecular weight of the heparosan capsular polysaccharide.
  2. Synthesis of different glycoforms is regulated by differential expression of WaaZ (KdoIII transferase) and WaaR (glycosyltransferase).
    Title: Molecular basis of lipopolysaccharide heterogeneity in Escherichia coli: envelope stress-responsive regulators control the incorporation of glycoforms with a third 3-deoxy-α-D-manno-oct-2-ulosonic acid and rhamnose.
  3. the C-terminal domain of WaaJ plays a critical role in enzyme function
    Title: The C-terminal Domain of the Escherichia coli WaaJ glycosyltransferase is important for catalytic activity and membrane association.
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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables glycosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables lipopolysaccharide 3-alpha-galactosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables lipopolysaccharide glucosyltransferase I activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables lipopolysaccharide glucosyltransferase I activity IEA
Inferred from Electronic Annotation
more info
  
enables lipopolysaccharide glucosyltransferase I activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in lipopolysaccharide biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in lipopolysaccharide core region biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within lipopolysaccharide core region biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in plasma membrane HDA PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
UDP-glucose:(glucosyl)LPS alpha-1,2-glucosyltransferase
NP_418083.1

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3800983..3801999 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418083.1 UDP-glucose:(glucosyl)LPS alpha-1,2-glucosyltransferase

    See identical proteins and their annotated locations for NP_418083.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A3R0MXW2
    Conserved Domains (1) summary
    COG1442
    Location:26 → 338
    RfaJ; Lipopolysaccharide biosynthesis protein, LPS:glycosyltransferase [Cell wall/membrane/envelope biogenesis]
Nucleotide Protein
Heading Accession and Version

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