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grxC reduced glutaredoxin 3 [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948132, updated on 29-Sep-2023

Summary

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Gene symbol
grxC
Gene description
reduced glutaredoxin 3
Primary source
ASAP:ABE-0011801
Locus tag
b3610
See related
ECOCYC:EG12294
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3600; yibM
Summary
There at least three glutaredoxins in Escherichia coli. [More information is available at EcoCyc: EG12294].
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Genomic context

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Sequence:
NC_000913.3 (3784191..3784442, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene serine acetyltransferase Neighboring gene glycerol-3-phosphate dehydrogenase Neighboring gene protein export chaperone SecB Neighboring gene putative sulfurtransferase YibN Neighboring gene 2,3-bisphosphoglycerate-independent phosphoglycerate mutase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Determinants of activity in glutaredoxins: an in vitro evolved Grx1-like variant of Escherichia coli Grx3. Elgán TH, et al. Biochem J, 2010 Sep 15. PMID 20604742
  2. Quantifying Escherichia coli glutaredoxin-3 substrate specificity using ligand-induced stability. Elgán TH, et al. J Biol Chem, 2008 Nov 21. PMID 18757366
  3. NMR structure of Escherichia coli glutaredoxin 3-glutathione mixed disulfide complex: implications for the enzymatic mechanism. Nordstrand K, et al. J Mol Biol, 1999 Feb 19. PMID 9973569
  4. The reducing activity of glutaredoxin 3 toward cytoplasmic substrate proteins is restricted by methionine 43. Porat A, et al. Biochemistry, 2007 Mar 20. PMID 17305371, Free PMC Article
  5. Glutaredoxin-3 from Escherichia coli. Amino acid sequence, 1H AND 15N NMR assignments, and structural analysis. Aslund F, et al. J Biol Chem, 1996 Mar 22. PMID 8636094

See all (22) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. data suggest that the Grx1-like activity of the Grx3(M43V/C65Y) mutant may be explained by enhanced intrinsic motion allowing for increased specificity towards larger substrates
    Title: Determinants of activity in glutaredoxins: an in vitro evolved Grx1-like variant of Escherichia coli Grx3.
  2. analysis of Escherichia coli glutaredoxin-3 substrate specificity
    Title: Quantifying Escherichia coli glutaredoxin-3 substrate specificity using ligand-induced stability.
  3. N-terminus verified by Edman degradation on mature peptide
    Title: Two additional glutaredoxins exist in Escherichia coli: glutaredoxin 3 is a hydrogen donor for ribonucleotide reductase in a thioredoxin/glutaredoxin 1 double mutant.
  4. Molecular dynamics simulations suggest that Met43Val substitution results in changes in properties of N-terminal cysteine of active site leading to considerably lower pKa. Grx3-Met43Val shows an 11 mV lower redox potential than the wild-type Grx3.
    Title: The reducing activity of glutaredoxin 3 toward cytoplasmic substrate proteins is restricted by methionine 43.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables glutathione binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables glutathione disulfide oxidoreductase activity IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
enables protein-disulfide reductase activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in cell redox homeostasis IEA
Inferred from Electronic Annotation
more info
  
involved_in cellular response to oxidative stress IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
involved_in deoxyribonucleotide biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
 PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
reduced glutaredoxin 3
NP_418067.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3784191..3784442 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418067.1 reduced glutaredoxin 3 [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418067.1

    Status: PROVISIONAL

    UniProtKB/Swiss-Prot
    P0AC62
    UniProtKB/TrEMBL
    A0A346Y7J1
    Conserved Domains (1) summary
    PRK10638
    Location:1 → 83
    PRK10638; glutaredoxin 3; Provisional
Nucleotide Protein
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