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malS alpha-amylase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948088, updated on 26-Mar-2025
Official Symbol
malS
Official Full Name
alpha-amylase
Primary source
ECOCYC:EG11316
Locus tag
b3571
See related
ASAP:ABE-0011668
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3560
Summary
MalT regulon. [More information is available at EcoGene: EG11316]. MalS is a periplasmic enzyme that degrades linear dextrins of at least three glucose residues. [More information is available at EcoCyc: EG11316].
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Sequence:
NC_000913.3 (3737497..3739527)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA-binding transcriptional dual regulator XylR Neighboring gene putative glycoside hydrolase Bax Neighboring gene putative translational regulatory protein BaxL Neighboring gene valine--pyruvate aminotransferase Neighboring gene putative electron transport protein YsaA

Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Related articles in PubMed

  1. Biochemical characterization and mass spectrometric disulfide bond mapping of periplasmic alpha-amylase MalS of Escherichia coli. Spiess C, et al. J Biol Chem, 1997 Aug 29. PMID 9268356
  2. Facilitated diffusion of p-nitrophenyl-alpha-D-maltohexaoside through the outer membrane of Escherichia coli. Characterization of LamB as a specific and saturable channel for maltooligosaccharides. Freundlieb S, et al. J Biol Chem, 1988 Jan 5. PMID 3275641
  3. Alpha-amylase of Escherichia coli, mapping and cloning of the structural gene, malS, and identification of its product as a periplasmic protein. Freundlieb S, et al. J Biol Chem, 1986 Feb 25. PMID 3005273
  4. Cloning and characterization of the Escherichia coli K-12 alanine-valine transaminase (avtA) gene. Wang MD, et al. J Bacteriol, 1987 Sep. PMID 3040683, Free PMC Article
  5. Molecular characterization of the MalT-dependent periplasmic alpha-amylase of Escherichia coli encoded by malS. Schneider E, et al. J Biol Chem, 1992 Mar 15. PMID 1544897

See all (14) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. N-terminus verified by Edman degradation on mature peptide
    Title: Molecular characterization of the MalT-dependent periplasmic alpha-amylase of Escherichia coli encoded by malS.
Submit: New GeneRIF Correction

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables alpha-amylase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables alpha-amylase activity IEA
Inferred from Electronic Annotation
more info
  
enables calcium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables calcium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity, acting on glycosyl bonds IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in alpha-glucan catabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within alpha-glucan catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in carbohydrate metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in oligosaccharide catabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in outer membrane-bounded periplasmic space IDA
Inferred from Direct Assay
more info
 PubMed 
located_in periplasmic space IDA
Inferred from Direct Assay
more info
 PubMed 
located_in periplasmic space IEA
Inferred from Electronic Annotation
more info
  
Preferred Names
alpha-amylase

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3737497..3739527
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_418028.1 alpha-amylase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_418028.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    Q5RLZ2
    Conserved Domains (1) summary
    PRK09505
    Location:1 → 676
    malS; alpha-amylase; Reviewed
Nucleotide Protein
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Gene LinkOut

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