cspA [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

Go to the top of the page Help

Official Symbol: cspA
Primary source: ECOCYC:EG10166
Locus tag: b3556
See related: ASAP:ABE-0011616
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK3543
Summary: The cspABEG deletion BX04 is cold-sensitive and forms filaments at 15C (Xia, 2001). [More information is available at EcoGene: EG10166]. CspA is an RNA chaperone that prevents secondary structure formation in RNA at low temperatures . [More information is available at EcoCyc: EG10166].
NEW: Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help

Sequence:: NC_000913.3 (3720049..3720261)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A switch from alpha-helical to beta-strand conformation during co-translational protein folding.
Title: A switch from α-helical to β-strand conformation during co-translational protein folding.
A Revised Understanding of Clostridioides difficile Spore Germination.
Title: A Revised Understanding of Clostridioides difficile Spore Germination.
Study showed improved drought tolerance in wheat plants overexpressing a synthetic bacterial cold shock protein gene SeCspA.
Title: Improved drought tolerance in wheat plants overexpressing a synthetic bacterial cold shock protein gene SeCspA.
the RNA-melting activity of CspA upon binding to different RNA hairpins was determined by NMR spectroscopy
Title: RNA binding and chaperone activity of the E. coli cold-shock protein CspA.
Overexpression of both 5'-UTR and truncated CspA mutant proteins caused the LACE (low temperature-dependent antibiotic effect of truncated cspA expression) phenotype, and polysomes appeared to be stalled at stop codons.
Title: Identification of two DNA helicases UvrD and DinG as suppressors for lethality caused by mutant cspA mRNAs.
Data propose that Hfq may play a facilitating role in the metabolism of cspA mRNA.
Title: Interactions of the RNA-binding protein Hfq with cspA mRNA, encoding the major cold shock protein.
N-terminus verified by Edman degradation on mature peptide
Title: Major cold shock protein of Escherichia coli.
Data suggest a model for the regulation of expression of CspA that rationalizes the roles of the secondary structure and RNase E.
Title: Role of RNA structure and susceptibility to RNase E in regulation of a cold shock mRNA, cspA mRNA.

Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

Go to the top of the page Help

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables nucleic acid binding	IBA Inferred from Biological aspect of Ancestor more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables transcription antitermination factor activity, RNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within negative regulation of termination of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of gene expression	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within response to cold	IEP Inferred from Expression Pattern more info	PubMed
involved_in transcription antitermination	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info

General protein information

Go to the top of the page Help

Preferred Names: cold shock protein CspA

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

3720049..3720261

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_418012.1 cold shock protein CspA [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_418012.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A8S0FDV4

Conserved Domains (1) summary

PRK10354
Location:1 → 70

PRK10354; RNA chaperone/antiterminator CspA